Characterisation of myofibroblasts in fibrovascular tissues of primary and recurrent pterygia

A. Touhami, M. A. Di Pascuale, T. Kawatika, M. Del Valle, R. H. Rosa, Sander Dubovy, Scheffer C G Tseng

Research output: Contribution to journalArticle

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Abstract

Aims: To determine the presence and origin of myofibroblasts in pterygia. Methods: 86 specimens including head, body, and fibrovascular tissue from 52 primary and 34 recurrent pterygia and five exenterated eyes without pterygia were searched for the origin of myofibroblasts. All tissues were subjected to haematoxylin and eosin staining, immunohistochemistry using antibodies against alpha smooth muscle actin (α-SMA), desmin, vimentin, and caldesmon, and transmission electron microscopy (TEM). The phenotype of fibroblasts subcultured in a serum free medium from pterygium fibrovascular tissues was characterised by the above antibodies. Bundles of dense fibrous tissues were noted in 86% of the fibrovascular tissue specimens evaluated. Cells within these bundles were characterised as myofibroblasts based on positive staining to α-SMA, but negative to desmin and caldesmon, markers for smooth muscle cells. Interestingly, positive α-SMA staining was also found in the periorbital fibroadipose tissue posterior to Tenon's capsule near the nasal conjunctiva in all exenterated specimens. All first passage fibroblasts expressed vimentin, some were positive to α-SMA, but all were negative to desmin or caldesmon. Cells in pterygium fibrovascular tissues showed ultrastructural features of intracytoplasmic bundles of microfilaments, consistent with myofibroblastic differentiation. Conclusion: These studies collectively demonstrate the presence of contractile myofibroblasts bundle in pterygia and in the periorbital fibroadipose tissue posterior to Tenon's capsule of exenterated eyes without pterygium.

Original languageEnglish
Pages (from-to)269-274
Number of pages6
JournalBritish Journal of Ophthalmology
Volume89
Issue number3
DOIs
StatePublished - Mar 1 2005

Fingerprint

Pterygium
Myofibroblasts
Calmodulin-Binding Proteins
Desmin
Tenon Capsule
Vimentin
Staining and Labeling
Fibroblasts
Antibodies
Conjunctiva
Serum-Free Culture Media
Hematoxylin
Eosine Yellowish-(YS)
Transmission Electron Microscopy
Actin Cytoskeleton
Nose
Smooth Muscle Myocytes
Smooth Muscle
Actins
Immunohistochemistry

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Touhami, A., Di Pascuale, M. A., Kawatika, T., Del Valle, M., Rosa, R. H., Dubovy, S., & Tseng, S. C. G. (2005). Characterisation of myofibroblasts in fibrovascular tissues of primary and recurrent pterygia. British Journal of Ophthalmology, 89(3), 269-274. https://doi.org/10.1136/bjo.2004.050633

Characterisation of myofibroblasts in fibrovascular tissues of primary and recurrent pterygia. / Touhami, A.; Di Pascuale, M. A.; Kawatika, T.; Del Valle, M.; Rosa, R. H.; Dubovy, Sander; Tseng, Scheffer C G.

In: British Journal of Ophthalmology, Vol. 89, No. 3, 01.03.2005, p. 269-274.

Research output: Contribution to journalArticle

Touhami, A, Di Pascuale, MA, Kawatika, T, Del Valle, M, Rosa, RH, Dubovy, S & Tseng, SCG 2005, 'Characterisation of myofibroblasts in fibrovascular tissues of primary and recurrent pterygia', British Journal of Ophthalmology, vol. 89, no. 3, pp. 269-274. https://doi.org/10.1136/bjo.2004.050633
Touhami, A. ; Di Pascuale, M. A. ; Kawatika, T. ; Del Valle, M. ; Rosa, R. H. ; Dubovy, Sander ; Tseng, Scheffer C G. / Characterisation of myofibroblasts in fibrovascular tissues of primary and recurrent pterygia. In: British Journal of Ophthalmology. 2005 ; Vol. 89, No. 3. pp. 269-274.
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