Chapter 16 Tagging for Protein Expression

Research output: Contribution to journalArticle

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Abstract

Tags are frequently used in the expression of recombinant proteins to improve solubility and for affinity purification. A large number of tags have been developed for protein production and researchers face a profusion of choices when designing expression constructs. Here, we survey common affinity and solubility tags, and offer some guidance on their selection and use.

Original languageEnglish
Pages (from-to)239-258
Number of pages20
JournalMethods in Enzymology
Volume463
Issue numberC
DOIs
StatePublished - Dec 14 2009

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Solubility
Recombinant Proteins
Purification
Proteins
Research Personnel
Surveys and Questionnaires

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Chapter 16 Tagging for Protein Expression. / Malhotra, Arun.

In: Methods in Enzymology, Vol. 463, No. C, 14.12.2009, p. 239-258.

Research output: Contribution to journalArticle

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