Chapter 16 Tagging for Protein Expression

Research output: Contribution to journalArticle

84 Scopus citations

Abstract

Tags are frequently used in the expression of recombinant proteins to improve solubility and for affinity purification. A large number of tags have been developed for protein production and researchers face a profusion of choices when designing expression constructs. Here, we survey common affinity and solubility tags, and offer some guidance on their selection and use.

Original languageEnglish
Pages (from-to)239-258
Number of pages20
JournalMethods in Enzymology
Volume463
Issue numberC
DOIs
StatePublished - Dec 14 2009

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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