Abstract
Proteins are fragile molecules that often require great care during purification to ensure that they remain intact and fully active. Nowadays, many proteins are also purified in small amounts under denaturing conditions by various gel electrophoretic techniques, such that inactive proteins are obtained. But even here, it is usually advantageous to maintain the protein in an intact form. In the case of enzymes, and other proteins with assayable biological activities, maintenance of activity is generally of prime importance, both for following the protein during purification and for subsequent studies of function. This chapter will focus on the major points to keep in mind with regard to maintaining the stability of a protein during purification and storage. Various other chapters describe in detail stabilization procedures for specific biological systems and specific classes of proteins.
| Original language | English |
|---|---|
| Pages (from-to) | 121-127 |
| Number of pages | 7 |
| Journal | Methods in Enzymology |
| Volume | 463 |
| Issue number | C |
| DOIs | |
| State | Published - Dec 14 2009 |
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ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
Cite this
Chapter 10 Maintaining Protein Stability. / Deutscher, Murray P.
In: Methods in Enzymology, Vol. 463, No. C, 14.12.2009, p. 121-127.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chapter 10 Maintaining Protein Stability
AU - Deutscher, Murray P
PY - 2009/12/14
Y1 - 2009/12/14
N2 - Proteins are fragile molecules that often require great care during purification to ensure that they remain intact and fully active. Nowadays, many proteins are also purified in small amounts under denaturing conditions by various gel electrophoretic techniques, such that inactive proteins are obtained. But even here, it is usually advantageous to maintain the protein in an intact form. In the case of enzymes, and other proteins with assayable biological activities, maintenance of activity is generally of prime importance, both for following the protein during purification and for subsequent studies of function. This chapter will focus on the major points to keep in mind with regard to maintaining the stability of a protein during purification and storage. Various other chapters describe in detail stabilization procedures for specific biological systems and specific classes of proteins.
AB - Proteins are fragile molecules that often require great care during purification to ensure that they remain intact and fully active. Nowadays, many proteins are also purified in small amounts under denaturing conditions by various gel electrophoretic techniques, such that inactive proteins are obtained. But even here, it is usually advantageous to maintain the protein in an intact form. In the case of enzymes, and other proteins with assayable biological activities, maintenance of activity is generally of prime importance, both for following the protein during purification and for subsequent studies of function. This chapter will focus on the major points to keep in mind with regard to maintaining the stability of a protein during purification and storage. Various other chapters describe in detail stabilization procedures for specific biological systems and specific classes of proteins.
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UR - http://www.scopus.com/inward/citedby.url?scp=71549160819&partnerID=8YFLogxK
U2 - 10.1016/S0076-6879(09)63010-X
DO - 10.1016/S0076-6879(09)63010-X
M3 - Article
C2 - 19892170
AN - SCOPUS:71549160819
VL - 463
SP - 121
EP - 127
JO - Methods in Enzymology
JF - Methods in Enzymology
SN - 0076-6879
IS - C
ER -