Changes in the GEF-H1 pathways after traumatic brain injury

Inna Sabirzhanova, Chunli Liu, Jingwei Zhao, Helen Bramlett, W. Dalton Dietrich, Bingren Hu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Brains undergo significant remodeling after traumatic brain injury (TBI). The Rho guanine triphosphate (GTP)ase pathways control brain remodeling during development and under pathological conditions. How the Rho GTPase pathways are regulated in the brain after TBI remains largely unknown, however. This study used the rat fluid percussion injury model to investigate changes in the Rho GTPase pathways after TBI. The results showed that TBI leads to activation and translocation of RhoA and Rac1 proteins from cytosolic fraction to the membrane fraction after injury. Consistently, the Rho guanine nucleotide exchange factors GEF-H1 and Cool-2/αPix are significantly activated by dephosphorylation and accumulation in the cytosolic fractions during the post-TBI phase. Because the Rho GTPase pathways are key regulators of brain remodeling, these results depict regulatory mechanisms of the Rho GTPase pathways after TBI, and pave the way for the study of therapeutic agents targeting the Rho GTPase pathways for functional recovery after TBI.

Original languageEnglish
Pages (from-to)1449-1456
Number of pages8
JournalJournal of Neurotrauma
Volume30
Issue number16
DOIs
StatePublished - Aug 15 2013

Fingerprint

rho GTP-Binding Proteins
Brain
rac1 GTP-Binding Protein
rhoA GTP-Binding Protein
Rho Guanine Nucleotide Exchange Factors
Percussion
Wounds and Injuries
Guanine
Traumatic Brain Injury
Membranes

Keywords

  • brain remodeling
  • Cool2/αPix
  • GEF-H1
  • Rho GTPases
  • traumatic brain injury

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Changes in the GEF-H1 pathways after traumatic brain injury. / Sabirzhanova, Inna; Liu, Chunli; Zhao, Jingwei; Bramlett, Helen; Dalton Dietrich, W.; Hu, Bingren.

In: Journal of Neurotrauma, Vol. 30, No. 16, 15.08.2013, p. 1449-1456.

Research output: Contribution to journalArticle

Sabirzhanova, Inna ; Liu, Chunli ; Zhao, Jingwei ; Bramlett, Helen ; Dalton Dietrich, W. ; Hu, Bingren. / Changes in the GEF-H1 pathways after traumatic brain injury. In: Journal of Neurotrauma. 2013 ; Vol. 30, No. 16. pp. 1449-1456.
@article{cf0b25cbf40547df8a794e8599b99a12,
title = "Changes in the GEF-H1 pathways after traumatic brain injury",
abstract = "Brains undergo significant remodeling after traumatic brain injury (TBI). The Rho guanine triphosphate (GTP)ase pathways control brain remodeling during development and under pathological conditions. How the Rho GTPase pathways are regulated in the brain after TBI remains largely unknown, however. This study used the rat fluid percussion injury model to investigate changes in the Rho GTPase pathways after TBI. The results showed that TBI leads to activation and translocation of RhoA and Rac1 proteins from cytosolic fraction to the membrane fraction after injury. Consistently, the Rho guanine nucleotide exchange factors GEF-H1 and Cool-2/αPix are significantly activated by dephosphorylation and accumulation in the cytosolic fractions during the post-TBI phase. Because the Rho GTPase pathways are key regulators of brain remodeling, these results depict regulatory mechanisms of the Rho GTPase pathways after TBI, and pave the way for the study of therapeutic agents targeting the Rho GTPase pathways for functional recovery after TBI.",
keywords = "brain remodeling, Cool2/αPix, GEF-H1, Rho GTPases, traumatic brain injury",
author = "Inna Sabirzhanova and Chunli Liu and Jingwei Zhao and Helen Bramlett and {Dalton Dietrich}, W. and Bingren Hu",
year = "2013",
month = "8",
day = "15",
doi = "10.1089/neu.2012.2673",
language = "English",
volume = "30",
pages = "1449--1456",
journal = "Journal of Neurotrauma",
issn = "0897-7151",
publisher = "Mary Ann Liebert Inc.",
number = "16",

}

TY - JOUR

T1 - Changes in the GEF-H1 pathways after traumatic brain injury

AU - Sabirzhanova, Inna

AU - Liu, Chunli

AU - Zhao, Jingwei

AU - Bramlett, Helen

AU - Dalton Dietrich, W.

AU - Hu, Bingren

PY - 2013/8/15

Y1 - 2013/8/15

N2 - Brains undergo significant remodeling after traumatic brain injury (TBI). The Rho guanine triphosphate (GTP)ase pathways control brain remodeling during development and under pathological conditions. How the Rho GTPase pathways are regulated in the brain after TBI remains largely unknown, however. This study used the rat fluid percussion injury model to investigate changes in the Rho GTPase pathways after TBI. The results showed that TBI leads to activation and translocation of RhoA and Rac1 proteins from cytosolic fraction to the membrane fraction after injury. Consistently, the Rho guanine nucleotide exchange factors GEF-H1 and Cool-2/αPix are significantly activated by dephosphorylation and accumulation in the cytosolic fractions during the post-TBI phase. Because the Rho GTPase pathways are key regulators of brain remodeling, these results depict regulatory mechanisms of the Rho GTPase pathways after TBI, and pave the way for the study of therapeutic agents targeting the Rho GTPase pathways for functional recovery after TBI.

AB - Brains undergo significant remodeling after traumatic brain injury (TBI). The Rho guanine triphosphate (GTP)ase pathways control brain remodeling during development and under pathological conditions. How the Rho GTPase pathways are regulated in the brain after TBI remains largely unknown, however. This study used the rat fluid percussion injury model to investigate changes in the Rho GTPase pathways after TBI. The results showed that TBI leads to activation and translocation of RhoA and Rac1 proteins from cytosolic fraction to the membrane fraction after injury. Consistently, the Rho guanine nucleotide exchange factors GEF-H1 and Cool-2/αPix are significantly activated by dephosphorylation and accumulation in the cytosolic fractions during the post-TBI phase. Because the Rho GTPase pathways are key regulators of brain remodeling, these results depict regulatory mechanisms of the Rho GTPase pathways after TBI, and pave the way for the study of therapeutic agents targeting the Rho GTPase pathways for functional recovery after TBI.

KW - brain remodeling

KW - Cool2/αPix

KW - GEF-H1

KW - Rho GTPases

KW - traumatic brain injury

UR - http://www.scopus.com/inward/record.url?scp=84881410704&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881410704&partnerID=8YFLogxK

U2 - 10.1089/neu.2012.2673

DO - 10.1089/neu.2012.2673

M3 - Article

C2 - 23611588

AN - SCOPUS:84881410704

VL - 30

SP - 1449

EP - 1456

JO - Journal of Neurotrauma

JF - Journal of Neurotrauma

SN - 0897-7151

IS - 16

ER -