Changes in pH(i) associated with activation of ion secretion in avian nasal salt gland cells

T. J. Shuttleworth, C. M. Wood

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The fluorescent pH-sensitive dye 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) was used to determine changes in intracellular pH (pH(i)) associated with activation of secretion in isolated cells from the salt-secreting avian nasal gland. A correction procedure overcoming artifacts due to BCECF leakage is described. Resting pH(i) averaged 7.15 ± 0.03 and was unaffected by the nominal removal of medium HCO3- or by the addition of the anion-exchange inhibitor 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) but was significantly reduced by amiloride (7.07 ± 0.02). Muscarinic activation of secretion resulted in a rapid intracellular acidification that was compensated by mechanisms which raised pH(i) to restore approximately resting levels within 5 min. The principal mechanism involved was amiloride-sensitive and independent of any sustained intracellular Ca2+ concentration change. Recovery of pH(i) was also aided by HCO3--dependent and DIDS-sensitive mechanisms not seen in the resting cell. The direction of the latter was pH(i)-dependent, with DIDS further decreasing pH(i) in acidified cells and increasing pH(i) in alkalinized cells. This suggests that the DIDS-sensitive pathways are activated under conditions where pH(i) has been shifted away from resting levels in either direction and act primarily to restore resting pH(i).

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume262
Issue number1 31-1
StatePublished - Jan 1 1992

Fingerprint

Salt Gland
Nose
Salts
Amiloride
Chemical activation
Ions
Acids
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
Acidification
Cholinergic Agents
Anions
Recovery
Direction compound
6-carboxyfluorescein
Artifacts

Keywords

  • 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein
  • Amiloride 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid
  • Anion exchange
  • Exocrine secretion
  • Intracellular calcium concentration
  • Muscarinic receptors
  • Sodium-hydrogen exchange

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology
  • Medicine(all)

Cite this

Changes in pH(i) associated with activation of ion secretion in avian nasal salt gland cells. / Shuttleworth, T. J.; Wood, C. M.

In: American Journal of Physiology - Cell Physiology, Vol. 262, No. 1 31-1, 01.01.1992.

Research output: Contribution to journalArticle

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