Changes in myofibrils and cytoskeleton of neonatal hamster myocardial cells in culture: An immunofluorescence study

C. Zhang, H. E. Osinska, S. L. Lemanski, X. P. Huang, L. F. Lemanski

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Myocardial cells in culture offer many possibilities for studying cellular and molecular biology of cardiac muscles. However, it is important to know how long these cells can be maintained in vitro without significant structural and biochemical changes. In this study, we have investigated the morphological changes of myofibril proteins and cytoskeletons by using immunofluorescent techniques in cultured neonatal hamster myocardial cells at different culture durations. Our results have demonstrated that these cultured cells still contain intact myofibrils and cytoskeletal proteins after 6 days in vitro incubation, however, the organization of some of these proteins is altered. The proteins most sensitive to these in vitro conditions are: myosin heavy chain, actin and desmin. The data indicate that the duration of the culture and the contractile activity of the myocardial cells in culture can influence organization of their contractile apparatus and cytoskeleton.

Original languageEnglish (US)
Pages (from-to)435-445
Number of pages11
JournalTissue and Cell
Volume37
Issue number6
DOIs
StatePublished - Dec 2005

Keywords

  • Hamster
  • Immunofluorescent microscopy
  • Myocardial cell
  • Myofibrils

ASJC Scopus subject areas

  • Insect Science
  • Cell Biology

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