Changes in cell surface glycoprotein expression during differentiation of human keratinocytes

C. Eberhard Klein, Carlos Cordon-Cardo, Rolf Soehnchen, Richard J. Cote, Herbert F. Oettgen, Magdalena Eisinger, Lloyd J. Old

Research output: Contribution to journalArticle

43 Scopus citations


Six cell surface glycoproteins defined by monoclonal antibodies were selected for study on human epidermal cells. In tests on tissue sections, three of the glycoproteins [ J143 (gp 140 30); T43 (gp 85 36); H99 (gp38)] were expressed in the basal cell layer of the epidermis, whereas the other three glycoproteins [T179 (gp 140 95); T16 (gp 40 50); BT15 (gp80)] were preferentially expressed in maturing keratinocytes above the basal layer. We compared synthesis of these glycoproteins in fresh epidermis and in primary epidermal short term cultures using [35S]methionine for metabolic labeling. Synthesis of J143 was 8- to 20-fold higher and synthesis of T43 was 4- to 10-fold lower in cultured cells compared with fresh epidermis. BT15, an antigen strongly expressed on terminally differentiating keratinocytes, was synthesized at 5- to 15-fold higher levels in fresh epidermis than in cultured cells. Biosynthesis levels of H99, T179, and T16 did not change in cultured epidermal cells. Based on our findings, we propose a model of surface antigenic changes that occur during keratinocyte differentiation in vivo.

Original languageEnglish (US)
Pages (from-to)500-506
Number of pages7
JournalJournal of Investigative Dermatology
Issue number5
StatePublished - Nov 1987

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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