Changes in cell surface glycoprotein expression during differentiation of human keratinocytes

C. Eberhard Klein, Carlos Cordon-Cardo, Rolf Soehnchen, Richard J Cote, Herbert F. Oettgen, Magdalena Eisinger, Lloyd J. Old

Research output: Contribution to journalArticle

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Abstract

Six cell surface glycoproteins defined by monoclonal antibodies were selected for study on human epidermal cells. In tests on tissue sections, three of the glycoproteins [ J143 (gp 140 30); T43 (gp 85 36); H99 (gp38)] were expressed in the basal cell layer of the epidermis, whereas the other three glycoproteins [T179 (gp 140 95); T16 (gp 40 50); BT15 (gp80)] were preferentially expressed in maturing keratinocytes above the basal layer. We compared synthesis of these glycoproteins in fresh epidermis and in primary epidermal short term cultures using [35S]methionine for metabolic labeling. Synthesis of J143 was 8- to 20-fold higher and synthesis of T43 was 4- to 10-fold lower in cultured cells compared with fresh epidermis. BT15, an antigen strongly expressed on terminally differentiating keratinocytes, was synthesized at 5- to 15-fold higher levels in fresh epidermis than in cultured cells. Biosynthesis levels of H99, T179, and T16 did not change in cultured epidermal cells. Based on our findings, we propose a model of surface antigenic changes that occur during keratinocyte differentiation in vivo.

Original languageEnglish
Pages (from-to)500-506
Number of pages7
JournalJournal of Investigative Dermatology
Volume89
Issue number5
StatePublished - Nov 1 1987
Externally publishedYes

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Membrane Glycoproteins
Keratinocytes
Epidermis
Glycoproteins
Cells
Cultured Cells
Biosynthesis
Methionine
Labeling
Monoclonal Antibodies
Tissue
Antigens

ASJC Scopus subject areas

  • Dermatology

Cite this

Klein, C. E., Cordon-Cardo, C., Soehnchen, R., Cote, R. J., Oettgen, H. F., Eisinger, M., & Old, L. J. (1987). Changes in cell surface glycoprotein expression during differentiation of human keratinocytes. Journal of Investigative Dermatology, 89(5), 500-506.

Changes in cell surface glycoprotein expression during differentiation of human keratinocytes. / Klein, C. Eberhard; Cordon-Cardo, Carlos; Soehnchen, Rolf; Cote, Richard J; Oettgen, Herbert F.; Eisinger, Magdalena; Old, Lloyd J.

In: Journal of Investigative Dermatology, Vol. 89, No. 5, 01.11.1987, p. 500-506.

Research output: Contribution to journalArticle

Klein, CE, Cordon-Cardo, C, Soehnchen, R, Cote, RJ, Oettgen, HF, Eisinger, M & Old, LJ 1987, 'Changes in cell surface glycoprotein expression during differentiation of human keratinocytes', Journal of Investigative Dermatology, vol. 89, no. 5, pp. 500-506.
Klein CE, Cordon-Cardo C, Soehnchen R, Cote RJ, Oettgen HF, Eisinger M et al. Changes in cell surface glycoprotein expression during differentiation of human keratinocytes. Journal of Investigative Dermatology. 1987 Nov 1;89(5):500-506.
Klein, C. Eberhard ; Cordon-Cardo, Carlos ; Soehnchen, Rolf ; Cote, Richard J ; Oettgen, Herbert F. ; Eisinger, Magdalena ; Old, Lloyd J. / Changes in cell surface glycoprotein expression during differentiation of human keratinocytes. In: Journal of Investigative Dermatology. 1987 ; Vol. 89, No. 5. pp. 500-506.
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