Changes in calcitonin gene RNA processing during growth of a human medullary thyroid carcinoma cell line

B. D. Nelkin, K. Y. Chen, A. De Bustros, B. A. Roos, S. B. Baylin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The ratios of calcitonin (CT) to calcitonin gene-related peptide (CGRP) mRNA, both generated by alternative RNA processing from the same primary RNA transcript, are shown by Northern blotting of cytoplasmic RNA to vary as a function of growth in a human medullary thyroid carcinoma cell line (TT). Upon initial seeding, CT mRNA levels are relatively high, and CGRP mRNA levels are relatively low. During the early logarithmic growth phase, CGRP mRNA levels rise severalfold, while CT mRNA levels change only slightly. As the cells approach confluence, both CT and CGRP mRNA levels rise. Subsequently, CGRP mRNA levels fall substantially in postconfluent cells, while CT mRNA levels remain high. By actinomycin D blocking of nascent transcription, we have shown that these growth-related, reversible changes in the ratio of CT to CGRP mRNA are not due to changes in mRNA stability. Our data rather suggest that TT cells reversibly alter alternative RNA-processing patterns dependent upon growth conditions in vitro, such that CT mRNA is lowest and CGRP mRNA is highest during rapid growth. The mechanisms underlying this RNA-processing alteration may play a role in certain patients with aggressive forms of medullary thyroid carcinoma, in whom a decrease or loss of CT levels heralds a poor prognosis.

Original languageEnglish
Pages (from-to)6949-6952
Number of pages4
JournalCancer Research
Volume49
Issue number24 I
StatePublished - Dec 1 1989
Externally publishedYes

Fingerprint

Calcitonin
Calcitonin Gene-Related Peptide
RNA
Cell Line
Messenger RNA
Growth
Genes
Medullary Thyroid cancer
RNA Precursors
RNA Stability
Dactinomycin
Northern Blotting

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Nelkin, B. D., Chen, K. Y., De Bustros, A., Roos, B. A., & Baylin, S. B. (1989). Changes in calcitonin gene RNA processing during growth of a human medullary thyroid carcinoma cell line. Cancer Research, 49(24 I), 6949-6952.

Changes in calcitonin gene RNA processing during growth of a human medullary thyroid carcinoma cell line. / Nelkin, B. D.; Chen, K. Y.; De Bustros, A.; Roos, B. A.; Baylin, S. B.

In: Cancer Research, Vol. 49, No. 24 I, 01.12.1989, p. 6949-6952.

Research output: Contribution to journalArticle

Nelkin, BD, Chen, KY, De Bustros, A, Roos, BA & Baylin, SB 1989, 'Changes in calcitonin gene RNA processing during growth of a human medullary thyroid carcinoma cell line', Cancer Research, vol. 49, no. 24 I, pp. 6949-6952.
Nelkin BD, Chen KY, De Bustros A, Roos BA, Baylin SB. Changes in calcitonin gene RNA processing during growth of a human medullary thyroid carcinoma cell line. Cancer Research. 1989 Dec 1;49(24 I):6949-6952.
Nelkin, B. D. ; Chen, K. Y. ; De Bustros, A. ; Roos, B. A. ; Baylin, S. B. / Changes in calcitonin gene RNA processing during growth of a human medullary thyroid carcinoma cell line. In: Cancer Research. 1989 ; Vol. 49, No. 24 I. pp. 6949-6952.
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