Changes in axon fluorescence during activity

Molecular probes of membrane potential

L. B. Cohen, B. M. Salzberg, H. V. Davila, W. N. Ross, David Landowne, A. S. Waggoner, C. H. Wang

Research output: Contribution to journalArticle

288 Citations (Scopus)

Abstract

The fluorescence of dyes added to squid giant axons was studied during action potentials and voltage-clamp steps. One goal was to find fluorescence changes related to the increases in membrane conductance that underlie propagation. A second goal was to find large changes in fluorescence that would allow optical monitoring of membrane potential in neurons and other cells. Attempts were made to measure fluorescence changes using over 300 different fluorescent molecules and positive results were obtained with more than half of these. No evidence was found that would relate, any of the fluorescence changes to the increases in membrane conductance that accompany depolarization; most, instead, were correlated with the changes in membrane potential. The fluorescence changes of several dyes were relatively large; the largest changes during an action potential were 10-3 of the resting intensity. They could be measured with a signal-to-noise ratio of better than 10:1 in a single sweep.

Original languageEnglish
Pages (from-to)1-36
Number of pages36
JournalThe Journal of Membrane Biology
Volume19
Issue number1
DOIs
StatePublished - Dec 1 1974
Externally publishedYes

Fingerprint

Molecular Probes
Membrane Potentials
Axons
Fluorescence
Action Potentials
Coloring Agents
Decapodiformes
Membranes
Signal-To-Noise Ratio
Neurons

ASJC Scopus subject areas

  • Physiology
  • Cell Biology
  • Biophysics

Cite this

Cohen, L. B., Salzberg, B. M., Davila, H. V., Ross, W. N., Landowne, D., Waggoner, A. S., & Wang, C. H. (1974). Changes in axon fluorescence during activity: Molecular probes of membrane potential. The Journal of Membrane Biology, 19(1), 1-36. https://doi.org/10.1007/BF01869968

Changes in axon fluorescence during activity : Molecular probes of membrane potential. / Cohen, L. B.; Salzberg, B. M.; Davila, H. V.; Ross, W. N.; Landowne, David; Waggoner, A. S.; Wang, C. H.

In: The Journal of Membrane Biology, Vol. 19, No. 1, 01.12.1974, p. 1-36.

Research output: Contribution to journalArticle

Cohen, LB, Salzberg, BM, Davila, HV, Ross, WN, Landowne, D, Waggoner, AS & Wang, CH 1974, 'Changes in axon fluorescence during activity: Molecular probes of membrane potential', The Journal of Membrane Biology, vol. 19, no. 1, pp. 1-36. https://doi.org/10.1007/BF01869968
Cohen LB, Salzberg BM, Davila HV, Ross WN, Landowne D, Waggoner AS et al. Changes in axon fluorescence during activity: Molecular probes of membrane potential. The Journal of Membrane Biology. 1974 Dec 1;19(1):1-36. https://doi.org/10.1007/BF01869968
Cohen, L. B. ; Salzberg, B. M. ; Davila, H. V. ; Ross, W. N. ; Landowne, David ; Waggoner, A. S. ; Wang, C. H. / Changes in axon fluorescence during activity : Molecular probes of membrane potential. In: The Journal of Membrane Biology. 1974 ; Vol. 19, No. 1. pp. 1-36.
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