Cervical dysplasia specimens of HIV-infected women more likely contain non -16 and -18 high risk HPV types: Implications for primary prevention

N. D. McKenzie, Erin Kobetz, Tulay Sengul, P. Ganjei-Azar, Isabella Rosa-Cunha, JoNell E Potter, A. Morishita, III A. Lucci, T. Guettouche, J. H. Hnatyszyn

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: There is growing evidence that HIV-infected women might have a different HPV type distribution in cervical dysplasia specimens as compared to the general population. This has implications for primary prevention. Objective: We aimed to obtain preliminary data on the human papillomavirus (HPV) genotypes prevalent in histological samples of HIV-infected women with CIN 3/ CIS of the cervix in Miami, Florida. Method: Retrospective data were collected on HIV-infected women referred to the UM-JMH colposcopy clinic between years 2000 and 2008. The histology slides of CIN3/CIS biopsies underwent pathological review and sections were cut from these archived specimens for HPV DNA extraction. HPV genotyping was then performed using the GeneSquare™ HPV genotyping assay. We report on our first set of 23 samples. Results: Eight high risk HPV (HR-HPV) types were detected. Types in decreasing order of frequency were 16, 35, 45, 52, 59, 31, 58, and 56. Most cases had multiple infections. HPV type 16 was the most common (45%) followed by HPV-35 and -45 with equal frequency (40%). No samples contained HPV-18 Conclusion: Our preliminary suggest that cervical dysplasia specimens of HIV-infected women more likely (55%) contain non-16 and non-18 high risk HPV types. We show that this held true for histologically confirmed severe dysplasia and carcinoma in situ. Epidemiological studies guide vaccine development, therefore HPV type prevalence in CIS and invasive cervical cancer among HIV-infected women should be more rigorously explored to ensure that this highly vulnerable population receives appropriate primary prevention.

Original languageEnglish
Article number179
JournalFrontiers in Oncology
Volume4 JUN
DOIs
StatePublished - Jan 1 2014

Fingerprint

Uterine Cervical Dysplasia
Primary Prevention
HIV
Human papillomavirus 18
Colposcopy
Human papillomavirus 16
Carcinoma in Situ
Human Development
Vulnerable Populations
Cervix Uteri
Uterine Cervical Neoplasms
Epidemiologic Studies
Histology
Vaccines
Genotype
Biopsy
DNA

Keywords

  • Cervical cancer
  • CIN
  • HIV
  • HPV

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Cervical dysplasia specimens of HIV-infected women more likely contain non -16 and -18 high risk HPV types : Implications for primary prevention. / McKenzie, N. D.; Kobetz, Erin; Sengul, Tulay; Ganjei-Azar, P.; Rosa-Cunha, Isabella; Potter, JoNell E; Morishita, A.; Lucci, III A.; Guettouche, T.; Hnatyszyn, J. H.

In: Frontiers in Oncology, Vol. 4 JUN, 179, 01.01.2014.

Research output: Contribution to journalArticle

@article{16d43ffd4c3e4001a6c6bcbe8f564b53,
title = "Cervical dysplasia specimens of HIV-infected women more likely contain non -16 and -18 high risk HPV types: Implications for primary prevention",
abstract = "Background: There is growing evidence that HIV-infected women might have a different HPV type distribution in cervical dysplasia specimens as compared to the general population. This has implications for primary prevention. Objective: We aimed to obtain preliminary data on the human papillomavirus (HPV) genotypes prevalent in histological samples of HIV-infected women with CIN 3/ CIS of the cervix in Miami, Florida. Method: Retrospective data were collected on HIV-infected women referred to the UM-JMH colposcopy clinic between years 2000 and 2008. The histology slides of CIN3/CIS biopsies underwent pathological review and sections were cut from these archived specimens for HPV DNA extraction. HPV genotyping was then performed using the GeneSquare™ HPV genotyping assay. We report on our first set of 23 samples. Results: Eight high risk HPV (HR-HPV) types were detected. Types in decreasing order of frequency were 16, 35, 45, 52, 59, 31, 58, and 56. Most cases had multiple infections. HPV type 16 was the most common (45{\%}) followed by HPV-35 and -45 with equal frequency (40{\%}). No samples contained HPV-18 Conclusion: Our preliminary suggest that cervical dysplasia specimens of HIV-infected women more likely (55{\%}) contain non-16 and non-18 high risk HPV types. We show that this held true for histologically confirmed severe dysplasia and carcinoma in situ. Epidemiological studies guide vaccine development, therefore HPV type prevalence in CIS and invasive cervical cancer among HIV-infected women should be more rigorously explored to ensure that this highly vulnerable population receives appropriate primary prevention.",
keywords = "Cervical cancer, CIN, HIV, HPV",
author = "McKenzie, {N. D.} and Erin Kobetz and Tulay Sengul and P. Ganjei-Azar and Isabella Rosa-Cunha and Potter, {JoNell E} and A. Morishita and Lucci, {III A.} and T. Guettouche and Hnatyszyn, {J. H.}",
year = "2014",
month = "1",
day = "1",
doi = "10.3389/fonc.2014.00179",
language = "English",
volume = "4 JUN",
journal = "Frontiers in Oncology",
issn = "2234-943X",
publisher = "Frontiers Media S. A.",

}

TY - JOUR

T1 - Cervical dysplasia specimens of HIV-infected women more likely contain non -16 and -18 high risk HPV types

T2 - Implications for primary prevention

AU - McKenzie, N. D.

AU - Kobetz, Erin

AU - Sengul, Tulay

AU - Ganjei-Azar, P.

AU - Rosa-Cunha, Isabella

AU - Potter, JoNell E

AU - Morishita, A.

AU - Lucci, III A.

AU - Guettouche, T.

AU - Hnatyszyn, J. H.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Background: There is growing evidence that HIV-infected women might have a different HPV type distribution in cervical dysplasia specimens as compared to the general population. This has implications for primary prevention. Objective: We aimed to obtain preliminary data on the human papillomavirus (HPV) genotypes prevalent in histological samples of HIV-infected women with CIN 3/ CIS of the cervix in Miami, Florida. Method: Retrospective data were collected on HIV-infected women referred to the UM-JMH colposcopy clinic between years 2000 and 2008. The histology slides of CIN3/CIS biopsies underwent pathological review and sections were cut from these archived specimens for HPV DNA extraction. HPV genotyping was then performed using the GeneSquare™ HPV genotyping assay. We report on our first set of 23 samples. Results: Eight high risk HPV (HR-HPV) types were detected. Types in decreasing order of frequency were 16, 35, 45, 52, 59, 31, 58, and 56. Most cases had multiple infections. HPV type 16 was the most common (45%) followed by HPV-35 and -45 with equal frequency (40%). No samples contained HPV-18 Conclusion: Our preliminary suggest that cervical dysplasia specimens of HIV-infected women more likely (55%) contain non-16 and non-18 high risk HPV types. We show that this held true for histologically confirmed severe dysplasia and carcinoma in situ. Epidemiological studies guide vaccine development, therefore HPV type prevalence in CIS and invasive cervical cancer among HIV-infected women should be more rigorously explored to ensure that this highly vulnerable population receives appropriate primary prevention.

AB - Background: There is growing evidence that HIV-infected women might have a different HPV type distribution in cervical dysplasia specimens as compared to the general population. This has implications for primary prevention. Objective: We aimed to obtain preliminary data on the human papillomavirus (HPV) genotypes prevalent in histological samples of HIV-infected women with CIN 3/ CIS of the cervix in Miami, Florida. Method: Retrospective data were collected on HIV-infected women referred to the UM-JMH colposcopy clinic between years 2000 and 2008. The histology slides of CIN3/CIS biopsies underwent pathological review and sections were cut from these archived specimens for HPV DNA extraction. HPV genotyping was then performed using the GeneSquare™ HPV genotyping assay. We report on our first set of 23 samples. Results: Eight high risk HPV (HR-HPV) types were detected. Types in decreasing order of frequency were 16, 35, 45, 52, 59, 31, 58, and 56. Most cases had multiple infections. HPV type 16 was the most common (45%) followed by HPV-35 and -45 with equal frequency (40%). No samples contained HPV-18 Conclusion: Our preliminary suggest that cervical dysplasia specimens of HIV-infected women more likely (55%) contain non-16 and non-18 high risk HPV types. We show that this held true for histologically confirmed severe dysplasia and carcinoma in situ. Epidemiological studies guide vaccine development, therefore HPV type prevalence in CIS and invasive cervical cancer among HIV-infected women should be more rigorously explored to ensure that this highly vulnerable population receives appropriate primary prevention.

KW - Cervical cancer

KW - CIN

KW - HIV

KW - HPV

UR - http://www.scopus.com/inward/record.url?scp=84904689774&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84904689774&partnerID=8YFLogxK

U2 - 10.3389/fonc.2014.00179

DO - 10.3389/fonc.2014.00179

M3 - Article

AN - SCOPUS:84904689774

VL - 4 JUN

JO - Frontiers in Oncology

JF - Frontiers in Oncology

SN - 2234-943X

M1 - 179

ER -