Cerebral phosphoinositide, triacylglycerol and energy metabolism during severe hypoxia and recovery

Masuhiro Ikeda, Raul Busto, Shinichi Yoshida, Mercedes Santiso, Elena Martinez, Myron D. Ginsberg

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

The cerebral concentrations of phosphatidylinositol 4-phosphate (PIP), phosphatidylinositol 4,5-biphosphate (PIP2), phosphatidic acid (PA), triacylglycerol (TAG) and free fatty acids (FFA), as well as cerebral energy metabolites, were measured in rats subjected to 10 min of hypoxia and subsequent recovery of 7 or 30 min duration. The experiments were carried out with control of physiological variables. Hypoxia (paO2 values of about 15 mm Hg) caused a decrease in PI, whereas PIP and PIP2 not change significantly. A two-fold increase of total FFA was noted, mainly comprising stearic and arachidonic acids TAG-arachidonate tended to increase, but the other species in TAG decreased. Adenosine triphosphate (ATP) and energy charge (EC) decreased slightly and there was a marked lactate accumulation. PA did not change throughout the experiment. With recover of 7 min duration, PI decreased further and total FFA continued to increase. TAG-arachidonate increased significantly. ATP remained depressed but EC recovered to the control range. Both tissue and plasma glucose increased. Tissue lactate remained elevated and systemic acidosis occurred. After a recovery period of 30 min, all lipids normalized and the energy state returned toward control. The data suggest that the phosphoinositide alterations during hypoxia are metabolically linked to changes in FFA and the lipid changes are accompanied by alterations in cerebral energy and carbohydrate metabolism. The selective increase in TAG-arachidonate may represent an incorporation of arachidonic acid into TAG, which may serve to reduce the free arachidonate acid level in the brain.

Original languageEnglish (US)
Pages (from-to)344-350
Number of pages7
JournalBrain Research
Volume459
Issue number2
DOIs
StatePublished - Sep 6 1988

Keywords

  • Energy metabolism
  • Free fatty acid
  • Hypoxia
  • Phosphoinositide
  • Triacylglycerol

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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