Abstract
Subcellular fractionation techniques are essential for cell biology and drug development studies. The emergence of organelle-targeted nanoparticle (NP) platforms necessitates the isolation of target organelles to study drug delivery and activity. Mitochondria-targeted NPs have attracted the attention of researchers around the globe, since mitochondrial dysfunctions can cause a wide range of diseases. Conventional mitochondria isolation methods involve high-speed centrifugation. The problem with high-speed centrifugation-based isolation of NP-loaded mitochondria is that NPs can pellet even if they are not bound to mitochondria. We report development of a mitochondria-targeted paramagnetic iron oxide nanoparticle, Mito-magneto, that enables isolation of mitochondria under the influence of a magnetic field. Isolation of mitochondria using Mito-magneto eliminates artifacts typically associated with centrifugation-based isolation of NP-loaded mitochondria, thus producing intact, pure, and respiration-active mitochondria.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 25.4.1-25.4.20 |
| Journal | Current Protocols in Cell Biology |
| Volume | 2017 |
| DOIs | |
| State | Published - Sep 1 2017 |
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Keywords
- Centrifugation-free method
- Iron oxide nanoparticles
- Magnetic resonance imaging
- Mitochondria isolation
ASJC Scopus subject areas
- Cell Biology
Cite this
Centrifugation-free magnetic isolation of functional mitochondria using paramagnetic iron oxide nanoparticles. / Banik, Bhabatosh; Dhar, Shanta.
In: Current Protocols in Cell Biology, Vol. 2017, 01.09.2017, p. 25.4.1-25.4.20.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Centrifugation-free magnetic isolation of functional mitochondria using paramagnetic iron oxide nanoparticles
AU - Banik, Bhabatosh
AU - Dhar, Shanta
PY - 2017/9/1
Y1 - 2017/9/1
N2 - Subcellular fractionation techniques are essential for cell biology and drug development studies. The emergence of organelle-targeted nanoparticle (NP) platforms necessitates the isolation of target organelles to study drug delivery and activity. Mitochondria-targeted NPs have attracted the attention of researchers around the globe, since mitochondrial dysfunctions can cause a wide range of diseases. Conventional mitochondria isolation methods involve high-speed centrifugation. The problem with high-speed centrifugation-based isolation of NP-loaded mitochondria is that NPs can pellet even if they are not bound to mitochondria. We report development of a mitochondria-targeted paramagnetic iron oxide nanoparticle, Mito-magneto, that enables isolation of mitochondria under the influence of a magnetic field. Isolation of mitochondria using Mito-magneto eliminates artifacts typically associated with centrifugation-based isolation of NP-loaded mitochondria, thus producing intact, pure, and respiration-active mitochondria.
AB - Subcellular fractionation techniques are essential for cell biology and drug development studies. The emergence of organelle-targeted nanoparticle (NP) platforms necessitates the isolation of target organelles to study drug delivery and activity. Mitochondria-targeted NPs have attracted the attention of researchers around the globe, since mitochondrial dysfunctions can cause a wide range of diseases. Conventional mitochondria isolation methods involve high-speed centrifugation. The problem with high-speed centrifugation-based isolation of NP-loaded mitochondria is that NPs can pellet even if they are not bound to mitochondria. We report development of a mitochondria-targeted paramagnetic iron oxide nanoparticle, Mito-magneto, that enables isolation of mitochondria under the influence of a magnetic field. Isolation of mitochondria using Mito-magneto eliminates artifacts typically associated with centrifugation-based isolation of NP-loaded mitochondria, thus producing intact, pure, and respiration-active mitochondria.
KW - Centrifugation-free method
KW - Iron oxide nanoparticles
KW - Magnetic resonance imaging
KW - Mitochondria isolation
UR - http://www.scopus.com/inward/record.url?scp=85030257493&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85030257493&partnerID=8YFLogxK
U2 - 10.1002/cpcb.26
DO - 10.1002/cpcb.26
M3 - Article
C2 - 28862341
AN - SCOPUS:85030257493
VL - 2017
SP - 25.4.1-25.4.20
JO - Current Protocols in Cell Biology
JF - Current Protocols in Cell Biology
SN - 1934-2500
ER -