Centrifugation-free magnetic isolation of functional mitochondria using paramagnetic iron oxide nanoparticles

Bhabatosh Banik, Shanta Dhar

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Subcellular fractionation techniques are essential for cell biology and drug development studies. The emergence of organelle-targeted nanoparticle (NP) platforms necessitates the isolation of target organelles to study drug delivery and activity. Mitochondria-targeted NPs have attracted the attention of researchers around the globe, since mitochondrial dysfunctions can cause a wide range of diseases. Conventional mitochondria isolation methods involve high-speed centrifugation. The problem with high-speed centrifugation-based isolation of NP-loaded mitochondria is that NPs can pellet even if they are not bound to mitochondria. We report development of a mitochondria-targeted paramagnetic iron oxide nanoparticle, Mito-magneto, that enables isolation of mitochondria under the influence of a magnetic field. Isolation of mitochondria using Mito-magneto eliminates artifacts typically associated with centrifugation-based isolation of NP-loaded mitochondria, thus producing intact, pure, and respiration-active mitochondria.

Original languageEnglish (US)
Pages (from-to)25.4.1-25.4.20
JournalCurrent Protocols in Cell Biology
Volume2017
DOIs
StatePublished - Sep 1 2017

Keywords

  • Centrifugation-free method
  • Iron oxide nanoparticles
  • Magnetic resonance imaging
  • Mitochondria isolation

ASJC Scopus subject areas

  • Cell Biology

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