The possible significance of high levels of γ-glutamyl transpeptidase (γ-GT) to the cellular transport of acivicin (NSC 163501; AT-125) by human pancreatic carcinoma cells (MIA PaCa-2) in culture was studied. Acivicin could not be substituted for glycylglycine in the γ-GT assay whereas other amino acids, to varying degrees, could be (i.e. glutamine). Amino acids such as leucine, DL-bicyclohexane, and phenylalanine reduced cellular uptake of acivicin by 50%. Inhibitors of γ-GT, azaserine and dioxonorleucine, did not affect acivicin uptake. It was concluded that acivicin, an irreversible inhibitor of γ-GT, is predominantly translocated into MIA PaCa-2 cells by the L-amino acid preferring pathway. A secondary pathway is via the ASC-system.
|Original language||English (US)|
|Number of pages||2|
|Journal||IRCS Medical Science|
|State||Published - Jan 1 1983|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)