Cellular LTB4 prodduction differs in allergic sheep with and without late airway responses

G. A. Chapman, F. Signoretti, I. T. Lauredo, P. Torrealba, M. W. Sielczak, A. Ahmed, W. M. Abraham

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


We tested the hypothesis that allergic sheep that develop both early and late airway responses to inhaled Ascaris suum antigen (late responders) have an increased capacity to generate leukotrienes (LTs) compared with allergic sheep that show only early responses to inhaled antigen (acute responders). To test this hypothesis, we measured LTB4 production, in vitro, by granulocytes isolated from peripheral blood and by macrophages isolated from bronchoalveolar lavage (BAL) from both groups of sheep ≥ 2 wk after the animal's last antigen challenge; LTB4 production by granulocytes isolated from BAL from both groups of sheep 6 and 48 h after local airway challenge with A. suum antigen was also measured. LTB4 production was induced by incubating cells (i.e., either granulocytes or macrophages) with calcium ionophore (A23187, 2 μM) and arachidonic acid (30 μM). LTB4 production was quantitated by high-performance liquid chromatography and verified by radioimmunoassay (RIA). On stimulation peripheral blood granulocytes from late responders (n = 7) produced (means ± SD/106 cells) 13.3 ± 5.2 ng LTB4 compared with 5.3 ± 1.5 ng LTB4 (P < 0.05) for acute responders (n = 7). This increased LTB4 production did not result from variations in granulocyte differential or cyclooxygenase activity (as indicated by RIA measurements of prostaglandin E2 production). Alveolar macrophages from late responders (n = 20) also produced more (P < 0.05) LTB4 (6.7 ± 2.8 ng) than did macrophages obtained from acute responders (4.6 ± 2.0 ng, n = 16). Lung granulocytes obtained 6 and 48 h after local antigen challenge from late responders produced 78 and 76% (both P < 0.05) more LTB4 than did granulocytes from acute responders at the respective measurement periods. The increased capacity of cells from dual responders to generate LTB4 may be one factor that contributes to the development of late airway responses in the allergic sheep model.

Original languageEnglish (US)
Pages (from-to)L136-L143
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Issue number2 3-1
StatePublished - 1990


  • 5-Lipoxygenase
  • Arachidonic acid metabolism
  • Asthma
  • Cyclooxygenase
  • Late responses
  • Leukotriene B

ASJC Scopus subject areas

  • Cell Biology
  • Physiology
  • Pulmonary and Respiratory Medicine


Dive into the research topics of 'Cellular LTB<sub>4</sub> prodduction differs in allergic sheep with and without late airway responses'. Together they form a unique fingerprint.

Cite this