Cellular localization of simian immunodeficiency virus in lymphoid tissues. II. In situ hybridization

M. S. Wyand, D. J. Ringler, Y. M. Naidu, M. Mattmuller, L. V. Chalifoux, P. K. Sehgal, M. D. Daniel, Ronald Charles Desrosiers, N. W. King

Research output: Contribution to journalArticle

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Abstract

Lymph nodes and spleens were collected at autopsy and by biopsy from 29 rhesus monkeys infected with simian immunodeficiency virus (SIV). Lymph nodes were classified morphologically into stages of follicular hyperplasia, follicular involution, follicular depletion with normal or expanded paracortices, follicular and paracortical depletion, granulomatous lymphadenitis, or normal. The distribution of SIV RNA was determined by in situ hybridization using a nick translated, 35S labeled, SIVmac DNA probe. Numbers of SIV-infected cells were rare during follicular hyperplasia, numerous during follicular depletion and paracortical expansion, and rare during follicular and paracortical depletion. The splenic morphology reflected that of the lymph nodes; however, the numbers of SIV-positive cells were uniformly lower. SIV RNA was frequently restricted to a single nucleus within multinucleate syncytial cells in two cases of granulomatous lymphadenitis. These results, combined with those of a previous study, provide evidence for antigen trapping in SIV-infected hyperplastic lymph nodes and for widespread viral infection of macrophages and lymphocytes during paracortical expansion.

Original languageEnglish (US)
Pages (from-to)385-393
Number of pages9
JournalAmerican Journal of Pathology
Volume134
Issue number2
StatePublished - 1989
Externally publishedYes

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Simian Immunodeficiency Virus
Lymphoid Tissue
In Situ Hybridization
Lymph Nodes
Lymphadenitis
Hyperplasia
RNA
DNA Probes
Virus Diseases
Macaca mulatta
Autopsy
Spleen
Macrophages
Lymphocytes
Biopsy
Antigens

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Wyand, M. S., Ringler, D. J., Naidu, Y. M., Mattmuller, M., Chalifoux, L. V., Sehgal, P. K., ... King, N. W. (1989). Cellular localization of simian immunodeficiency virus in lymphoid tissues. II. In situ hybridization. American Journal of Pathology, 134(2), 385-393.

Cellular localization of simian immunodeficiency virus in lymphoid tissues. II. In situ hybridization. / Wyand, M. S.; Ringler, D. J.; Naidu, Y. M.; Mattmuller, M.; Chalifoux, L. V.; Sehgal, P. K.; Daniel, M. D.; Desrosiers, Ronald Charles; King, N. W.

In: American Journal of Pathology, Vol. 134, No. 2, 1989, p. 385-393.

Research output: Contribution to journalArticle

Wyand, MS, Ringler, DJ, Naidu, YM, Mattmuller, M, Chalifoux, LV, Sehgal, PK, Daniel, MD, Desrosiers, RC & King, NW 1989, 'Cellular localization of simian immunodeficiency virus in lymphoid tissues. II. In situ hybridization', American Journal of Pathology, vol. 134, no. 2, pp. 385-393.
Wyand MS, Ringler DJ, Naidu YM, Mattmuller M, Chalifoux LV, Sehgal PK et al. Cellular localization of simian immunodeficiency virus in lymphoid tissues. II. In situ hybridization. American Journal of Pathology. 1989;134(2):385-393.
Wyand, M. S. ; Ringler, D. J. ; Naidu, Y. M. ; Mattmuller, M. ; Chalifoux, L. V. ; Sehgal, P. K. ; Daniel, M. D. ; Desrosiers, Ronald Charles ; King, N. W. / Cellular localization of simian immunodeficiency virus in lymphoid tissues. II. In situ hybridization. In: American Journal of Pathology. 1989 ; Vol. 134, No. 2. pp. 385-393.
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