Cellular localization of perforin 1 in murine cloned cytotoxic T lymphocytes

P. Groscurth, B. Y. Qiao, E. R. Podack, H. Hengartner

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Abstract

The localization of perforin 1 (P1) in cytotoxic cells was studied by immuno-electron microscopy by using a monospecific rabbit antiserum against highly purified mouse P1 and protein A gold as a second ligand. P1 was found in specific granules of cloned cytotoxic T lymphocytes (CTL). Within the granules, P1 antigen was localized in the fine granular matrix, whereas the vesicular compartment remained free of gold particles. The amount of P1 antigen detectable by immuno-electron microscopy varied between different CTL clones. CTL with NK-like activity had the highest level of P1 antigen. A cytotoxicity loss CTL mutant had no detectable P1 antigen, suggesting an important role of P1 during cell-mediated cytolysis. P1 antigen was undetectable also in bone marrow macrophages, indicating a different cytolytic mechanism of these cells.

Original languageEnglish (US)
Pages (from-to)2749-2752
Number of pages4
JournalJournal of Immunology
Volume138
Issue number9
StatePublished - Jan 1 1987

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Groscurth, P., Qiao, B. Y., Podack, E. R., & Hengartner, H. (1987). Cellular localization of perforin 1 in murine cloned cytotoxic T lymphocytes. Journal of Immunology, 138(9), 2749-2752.