Cellular localization of perforin 1 in murine cloned cytotoxic T lymphocytes

P. Groscurth, B. Y. Qiao, E. R. Podack, H. Hengartner

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The localization of perforin 1 (P1) in cytotoxic cells was studied by immuno-electron microscopy by using a monospecific rabbit antiserum against highly purified mouse P1 and protein A gold as a second ligand. P1 was found in specific granules of cloned cytotoxic T lymphocytes (CTL). Within the granules, P1 antigen was localized in the fine granular matrix, whereas the vesicular compartment remained free of gold particles. The amount of P1 antigen detectable by immuno-electron microscopy varied between different CTL clones. CTL with NK-like activity had the highest level of P1 antigen. A cytotoxicity loss CTL mutant had no detectable P1 antigen, suggesting an important role of P1 during cell-mediated cytolysis. P1 antigen was undetectable also in bone marrow macrophages, indicating a different cytolytic mechanism of these cells.

Original languageEnglish
Pages (from-to)2749-2752
Number of pages4
JournalJournal of Immunology
Volume138
Issue number9
StatePublished - Jan 1 1987
Externally publishedYes

Fingerprint

Perforin
Cytotoxic T-Lymphocytes
Antigens
Immunoelectron Microscopy
Gold
Staphylococcal Protein A
Immune Sera
Clone Cells
Bone Marrow
Macrophages
Rabbits
Ligands

ASJC Scopus subject areas

  • Immunology

Cite this

Groscurth, P., Qiao, B. Y., Podack, E. R., & Hengartner, H. (1987). Cellular localization of perforin 1 in murine cloned cytotoxic T lymphocytes. Journal of Immunology, 138(9), 2749-2752.

Cellular localization of perforin 1 in murine cloned cytotoxic T lymphocytes. / Groscurth, P.; Qiao, B. Y.; Podack, E. R.; Hengartner, H.

In: Journal of Immunology, Vol. 138, No. 9, 01.01.1987, p. 2749-2752.

Research output: Contribution to journalArticle

Groscurth, P, Qiao, BY, Podack, ER & Hengartner, H 1987, 'Cellular localization of perforin 1 in murine cloned cytotoxic T lymphocytes', Journal of Immunology, vol. 138, no. 9, pp. 2749-2752.
Groscurth P, Qiao BY, Podack ER, Hengartner H. Cellular localization of perforin 1 in murine cloned cytotoxic T lymphocytes. Journal of Immunology. 1987 Jan 1;138(9):2749-2752.
Groscurth, P. ; Qiao, B. Y. ; Podack, E. R. ; Hengartner, H. / Cellular localization of perforin 1 in murine cloned cytotoxic T lymphocytes. In: Journal of Immunology. 1987 ; Vol. 138, No. 9. pp. 2749-2752.
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