Abstract
There are currently 5 million to 10 million human T-lymphotropic virus type 1 (HTLV-1)-infected people, and many of them will develop severe complications resulting from this infection. A vaccine is urgently needed in areas where HTLV-1 is endemic. Many vaccines are best tested in nonhuman primate animal models. As a first step in designing an effective HTLV-1 vaccine, we defined the CD8+ and CD4+ T cell response against simian T-lymphotropic virus type 1 (STLV-1), a virus closely related to HTLV-1, in olive baboons (Papio anubis). Consistent with persistent antigenic exposure, we observed that STLV-1-specific CD8+ T cells displayed an effector memory phenotype and usually expressed CD107a, gamma interferon (IFN-γ), and tumor necrosis factor alpha (TNF-α). To assess the viral targets of the cellular immune response in STLV-1-infected animals, we used intracellular cytokine staining to detect responses against overlapping peptides covering the entire STLV-1 proteome. Our results show that, similarly to humans, the baboon CD8+ T cell response narrowly targeted the Tax protein. Our findings suggest that the STLV-1-infected baboon model may recapitulate some of the important aspects of the human response against HTLV-1 and could be an important tool for the development of immune-based therapy and prophylaxis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 5280-5291 |
| Number of pages | 12 |
| Journal | Journal of Virology |
| Volume | 90 |
| Issue number | 11 |
| DOIs | |
| State | Published - Jun 1 2016 |
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ASJC Scopus subject areas
- Immunology
- Virology
Cite this
Cellular immune responses against simian T-lymphotropic virus type 1 target Tax in infected baboons. / Castro, Iris; Giret, Teresa M.; Magnani, Diogo M.; Maxwell, Helen S.; Umland, Oliver; Perry, Jessica K.; Pecotte, Jerilyn K.; Brasky, Kathleen M.; Barber, Glen N; Desrosiers, Ronald Charles; Watkins, David.
In: Journal of Virology, Vol. 90, No. 11, 01.06.2016, p. 5280-5291.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cellular immune responses against simian T-lymphotropic virus type 1 target Tax in infected baboons
AU - Castro, Iris
AU - Giret, Teresa M.
AU - Magnani, Diogo M.
AU - Maxwell, Helen S.
AU - Umland, Oliver
AU - Perry, Jessica K.
AU - Pecotte, Jerilyn K.
AU - Brasky, Kathleen M.
AU - Barber, Glen N
AU - Desrosiers, Ronald Charles
AU - Watkins, David
PY - 2016/6/1
Y1 - 2016/6/1
N2 - There are currently 5 million to 10 million human T-lymphotropic virus type 1 (HTLV-1)-infected people, and many of them will develop severe complications resulting from this infection. A vaccine is urgently needed in areas where HTLV-1 is endemic. Many vaccines are best tested in nonhuman primate animal models. As a first step in designing an effective HTLV-1 vaccine, we defined the CD8+ and CD4+ T cell response against simian T-lymphotropic virus type 1 (STLV-1), a virus closely related to HTLV-1, in olive baboons (Papio anubis). Consistent with persistent antigenic exposure, we observed that STLV-1-specific CD8+ T cells displayed an effector memory phenotype and usually expressed CD107a, gamma interferon (IFN-γ), and tumor necrosis factor alpha (TNF-α). To assess the viral targets of the cellular immune response in STLV-1-infected animals, we used intracellular cytokine staining to detect responses against overlapping peptides covering the entire STLV-1 proteome. Our results show that, similarly to humans, the baboon CD8+ T cell response narrowly targeted the Tax protein. Our findings suggest that the STLV-1-infected baboon model may recapitulate some of the important aspects of the human response against HTLV-1 and could be an important tool for the development of immune-based therapy and prophylaxis.
AB - There are currently 5 million to 10 million human T-lymphotropic virus type 1 (HTLV-1)-infected people, and many of them will develop severe complications resulting from this infection. A vaccine is urgently needed in areas where HTLV-1 is endemic. Many vaccines are best tested in nonhuman primate animal models. As a first step in designing an effective HTLV-1 vaccine, we defined the CD8+ and CD4+ T cell response against simian T-lymphotropic virus type 1 (STLV-1), a virus closely related to HTLV-1, in olive baboons (Papio anubis). Consistent with persistent antigenic exposure, we observed that STLV-1-specific CD8+ T cells displayed an effector memory phenotype and usually expressed CD107a, gamma interferon (IFN-γ), and tumor necrosis factor alpha (TNF-α). To assess the viral targets of the cellular immune response in STLV-1-infected animals, we used intracellular cytokine staining to detect responses against overlapping peptides covering the entire STLV-1 proteome. Our results show that, similarly to humans, the baboon CD8+ T cell response narrowly targeted the Tax protein. Our findings suggest that the STLV-1-infected baboon model may recapitulate some of the important aspects of the human response against HTLV-1 and could be an important tool for the development of immune-based therapy and prophylaxis.
UR - http://www.scopus.com/inward/record.url?scp=84971468101&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84971468101&partnerID=8YFLogxK
U2 - 10.1128/JVI.00281-16
DO - 10.1128/JVI.00281-16
M3 - Article
C2 - 26984729
AN - SCOPUS:84971468101
VL - 90
SP - 5280
EP - 5291
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 11
ER -