Cellular immune responses against simian T-lymphotropic virus type 1 target Tax in infected baboons

Iris Castro, Teresa M. Giret, Diogo M. Magnani, Helen S. Maxwell, Oliver Umland, Jessica K. Perry, Jerilyn K. Pecotte, Kathleen M. Brasky, Glen N Barber, Ronald Charles Desrosiers, David Watkins

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

There are currently 5 million to 10 million human T-lymphotropic virus type 1 (HTLV-1)-infected people, and many of them will develop severe complications resulting from this infection. A vaccine is urgently needed in areas where HTLV-1 is endemic. Many vaccines are best tested in nonhuman primate animal models. As a first step in designing an effective HTLV-1 vaccine, we defined the CD8+ and CD4+ T cell response against simian T-lymphotropic virus type 1 (STLV-1), a virus closely related to HTLV-1, in olive baboons (Papio anubis). Consistent with persistent antigenic exposure, we observed that STLV-1-specific CD8+ T cells displayed an effector memory phenotype and usually expressed CD107a, gamma interferon (IFN-γ), and tumor necrosis factor alpha (TNF-α). To assess the viral targets of the cellular immune response in STLV-1-infected animals, we used intracellular cytokine staining to detect responses against overlapping peptides covering the entire STLV-1 proteome. Our results show that, similarly to humans, the baboon CD8+ T cell response narrowly targeted the Tax protein. Our findings suggest that the STLV-1-infected baboon model may recapitulate some of the important aspects of the human response against HTLV-1 and could be an important tool for the development of immune-based therapy and prophylaxis.

Original languageEnglish (US)
Pages (from-to)5280-5291
Number of pages12
JournalJournal of Virology
Volume90
Issue number11
DOIs
StatePublished - Jun 1 2016

Fingerprint

Simian T-lymphotropic virus 1
Human T-lymphotropic virus 1
Papio
taxes
Cellular Immunity
cell-mediated immunity
viruses
Papio anubis
Vaccines
T-Lymphocytes
tax Gene Products
Proteome
T-lymphocytes
vaccines
Primates
Interferon-gamma
Animal Models
Tumor Necrosis Factor-alpha
Staining and Labeling
Cytokines

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Castro, I., Giret, T. M., Magnani, D. M., Maxwell, H. S., Umland, O., Perry, J. K., ... Watkins, D. (2016). Cellular immune responses against simian T-lymphotropic virus type 1 target Tax in infected baboons. Journal of Virology, 90(11), 5280-5291. https://doi.org/10.1128/JVI.00281-16

Cellular immune responses against simian T-lymphotropic virus type 1 target Tax in infected baboons. / Castro, Iris; Giret, Teresa M.; Magnani, Diogo M.; Maxwell, Helen S.; Umland, Oliver; Perry, Jessica K.; Pecotte, Jerilyn K.; Brasky, Kathleen M.; Barber, Glen N; Desrosiers, Ronald Charles; Watkins, David.

In: Journal of Virology, Vol. 90, No. 11, 01.06.2016, p. 5280-5291.

Research output: Contribution to journalArticle

Castro, I, Giret, TM, Magnani, DM, Maxwell, HS, Umland, O, Perry, JK, Pecotte, JK, Brasky, KM, Barber, GN, Desrosiers, RC & Watkins, D 2016, 'Cellular immune responses against simian T-lymphotropic virus type 1 target Tax in infected baboons', Journal of Virology, vol. 90, no. 11, pp. 5280-5291. https://doi.org/10.1128/JVI.00281-16
Castro I, Giret TM, Magnani DM, Maxwell HS, Umland O, Perry JK et al. Cellular immune responses against simian T-lymphotropic virus type 1 target Tax in infected baboons. Journal of Virology. 2016 Jun 1;90(11):5280-5291. https://doi.org/10.1128/JVI.00281-16
Castro, Iris ; Giret, Teresa M. ; Magnani, Diogo M. ; Maxwell, Helen S. ; Umland, Oliver ; Perry, Jessica K. ; Pecotte, Jerilyn K. ; Brasky, Kathleen M. ; Barber, Glen N ; Desrosiers, Ronald Charles ; Watkins, David. / Cellular immune responses against simian T-lymphotropic virus type 1 target Tax in infected baboons. In: Journal of Virology. 2016 ; Vol. 90, No. 11. pp. 5280-5291.
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