Cellular immune responses against simian T-lymphotropic virus type 1 target Tax in infected baboons

Iris Castro, Teresa M. Giret, Diogo M. Magnani, Helen S. Maxwell, Oliver Umland, Jessica K. Perry, Jerilyn K. Pecotte, Kathleen M. Brasky, Glen N Barber, Ronald Charles Desrosiers, David Watkins

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

There are currently 5 million to 10 million human T-lymphotropic virus type 1 (HTLV-1)-infected people, and many of them will develop severe complications resulting from this infection. A vaccine is urgently needed in areas where HTLV-1 is endemic. Many vaccines are best tested in nonhuman primate animal models. As a first step in designing an effective HTLV-1 vaccine, we defined the CD8+ and CD4+ T cell response against simian T-lymphotropic virus type 1 (STLV-1), a virus closely related to HTLV-1, in olive baboons (Papio anubis). Consistent with persistent antigenic exposure, we observed that STLV-1-specific CD8+ T cells displayed an effector memory phenotype and usually expressed CD107a, gamma interferon (IFN-γ), and tumor necrosis factor alpha (TNF-α). To assess the viral targets of the cellular immune response in STLV-1-infected animals, we used intracellular cytokine staining to detect responses against overlapping peptides covering the entire STLV-1 proteome. Our results show that, similarly to humans, the baboon CD8+ T cell response narrowly targeted the Tax protein. Our findings suggest that the STLV-1-infected baboon model may recapitulate some of the important aspects of the human response against HTLV-1 and could be an important tool for the development of immune-based therapy and prophylaxis.

Original languageEnglish (US)
Pages (from-to)5280-5291
Number of pages12
JournalJournal of Virology
Volume90
Issue number11
DOIs
StatePublished - Jun 1 2016

ASJC Scopus subject areas

  • Immunology
  • Virology

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    Castro, I., Giret, T. M., Magnani, D. M., Maxwell, H. S., Umland, O., Perry, J. K., Pecotte, J. K., Brasky, K. M., Barber, G. N., Desrosiers, R. C., & Watkins, D. (2016). Cellular immune responses against simian T-lymphotropic virus type 1 target Tax in infected baboons. Journal of Virology, 90(11), 5280-5291. https://doi.org/10.1128/JVI.00281-16