Cells isolated from embryonic intestine synthesize 1,25-Dihydroxyvitamin-D3 and 24,25-dihydroxyvitamin-D3 in culture

J. Edward Puzas, Russell T. Turner, Guy A. Howard, David J. Baylinko

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Cells isolated from embryonic chick intestine in culture convert 25-hydroxyvitamin-D3 to a number of more polar metabolites. Two of these metabolites have been identified with chemical and chromatographic methods as 24,25-dihydroxyvitamin-D3 (24,25(OH)2D3) and 1,25-dihydroxyvitamin-D3 (1,25(OH)2D3). The enzymes conform to substrate saturation kinetics. The apparent Km for substrate for the synthesis of 1,25(OH)2D3 and 24,25(OH)2D3 is 70 nM and 29 nM, respectively.

Original languageEnglish (US)
Pages (from-to)378-380
Number of pages3
JournalEndocrinology
Volume112
Issue number1
DOIs
StatePublished - Jan 1983
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology

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