Cells expressing preproenkephalin mRNA in the rat pineal gland are not serotonin-producing pinealocytes

Evidence using in situ hybridization combined with immunocytochemistry for serotonin

Xi Tao Wang, George D. Pappas, Jacqueline Sagen, James R. Unnerstall

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

1. Preproenkephalin (PPEnk) mRNA expressing cells have been identified in rat pineal gland using radioactive in situ hybridization histochemistry. 2. Approximately 7% of the cells in the pineal gland (7.5 ± 0.86, mean ± 95% CI) express PPEnk mRNA. These cells are distributed throughout the pineal as either scattered single cells or small groups of cells with large round or oval nuclei. 3. Using in situ hybridization combined with ABC immunocytochemistry for serotonin (5-HT) in the same pineal sections, the PPEnk mRNA labeling cells are found not to be serotonin-immunoreactive cells. These data indicate that the PPEnk mRNA is expressed in a certain discrete subpopulation of cells in the rat pineal gland and these cells are not serotonin-producing pinealocytes. 4. The physiologic role of PPEnk-derived peptides in the pineal remains unknown. It is possible that these peptides either are synthesized and secreted as hormones or act as pineal paracrine signals.

Original languageEnglish
Pages (from-to)73-84
Number of pages12
JournalCellular and Molecular Neurobiology
Volume16
Issue number1
StatePublished - Feb 23 1996
Externally publishedYes

Fingerprint

Pineal Gland
In Situ Hybridization
Rats
Serotonin
Immunohistochemistry
Cells
Messenger RNA
Peptides
Labeling
preproenkephalin
Hormones

Keywords

  • double labeling
  • immunocytochemistry
  • in situ hybridization
  • pineal
  • preproenkephalin mRNA
  • serotonin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Biochemistry
  • Cell Biology
  • Genetics

Cite this

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title = "Cells expressing preproenkephalin mRNA in the rat pineal gland are not serotonin-producing pinealocytes: Evidence using in situ hybridization combined with immunocytochemistry for serotonin",
abstract = "1. Preproenkephalin (PPEnk) mRNA expressing cells have been identified in rat pineal gland using radioactive in situ hybridization histochemistry. 2. Approximately 7{\%} of the cells in the pineal gland (7.5 ± 0.86, mean ± 95{\%} CI) express PPEnk mRNA. These cells are distributed throughout the pineal as either scattered single cells or small groups of cells with large round or oval nuclei. 3. Using in situ hybridization combined with ABC immunocytochemistry for serotonin (5-HT) in the same pineal sections, the PPEnk mRNA labeling cells are found not to be serotonin-immunoreactive cells. These data indicate that the PPEnk mRNA is expressed in a certain discrete subpopulation of cells in the rat pineal gland and these cells are not serotonin-producing pinealocytes. 4. The physiologic role of PPEnk-derived peptides in the pineal remains unknown. It is possible that these peptides either are synthesized and secreted as hormones or act as pineal paracrine signals.",
keywords = "double labeling, immunocytochemistry, in situ hybridization, pineal, preproenkephalin mRNA, serotonin",
author = "Wang, {Xi Tao} and Pappas, {George D.} and Jacqueline Sagen and Unnerstall, {James R.}",
year = "1996",
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T2 - Evidence using in situ hybridization combined with immunocytochemistry for serotonin

AU - Wang, Xi Tao

AU - Pappas, George D.

AU - Sagen, Jacqueline

AU - Unnerstall, James R.

PY - 1996/2/23

Y1 - 1996/2/23

N2 - 1. Preproenkephalin (PPEnk) mRNA expressing cells have been identified in rat pineal gland using radioactive in situ hybridization histochemistry. 2. Approximately 7% of the cells in the pineal gland (7.5 ± 0.86, mean ± 95% CI) express PPEnk mRNA. These cells are distributed throughout the pineal as either scattered single cells or small groups of cells with large round or oval nuclei. 3. Using in situ hybridization combined with ABC immunocytochemistry for serotonin (5-HT) in the same pineal sections, the PPEnk mRNA labeling cells are found not to be serotonin-immunoreactive cells. These data indicate that the PPEnk mRNA is expressed in a certain discrete subpopulation of cells in the rat pineal gland and these cells are not serotonin-producing pinealocytes. 4. The physiologic role of PPEnk-derived peptides in the pineal remains unknown. It is possible that these peptides either are synthesized and secreted as hormones or act as pineal paracrine signals.

AB - 1. Preproenkephalin (PPEnk) mRNA expressing cells have been identified in rat pineal gland using radioactive in situ hybridization histochemistry. 2. Approximately 7% of the cells in the pineal gland (7.5 ± 0.86, mean ± 95% CI) express PPEnk mRNA. These cells are distributed throughout the pineal as either scattered single cells or small groups of cells with large round or oval nuclei. 3. Using in situ hybridization combined with ABC immunocytochemistry for serotonin (5-HT) in the same pineal sections, the PPEnk mRNA labeling cells are found not to be serotonin-immunoreactive cells. These data indicate that the PPEnk mRNA is expressed in a certain discrete subpopulation of cells in the rat pineal gland and these cells are not serotonin-producing pinealocytes. 4. The physiologic role of PPEnk-derived peptides in the pineal remains unknown. It is possible that these peptides either are synthesized and secreted as hormones or act as pineal paracrine signals.

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KW - in situ hybridization

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KW - preproenkephalin mRNA

KW - serotonin

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