Cell/cell channel formation involves disulfide exchange

Gerhard DAHL, Eric LEVINE, Cristina RABADAN‐DIEHL, Rudolf WERNER

Research output: Contribution to journalArticlepeer-review

61 Scopus citations


The oocyte cell/cell-channel assay was used to identify amino acids involved in the process of cell/cell-channel formation. The expression of the rat liver gap-junction protein, connexin 32, in single oocytes, results in the accumulation of a pool of channel precursors. Upon pairing of such oocytes, cell/cell channels form rapidly from this pool. The rate of formation is affected by thiol-specific reagents and the pH. This suggests the involvement of extracellular cysteine residues in the channel formation process. Two connexin-32 mutants were generated by site-directed mutagenesis in which cysteine residues were replaced by serine. Both mutant connexins were unable to form cell/cell channels. Thus, the cysteine residues appear to play an important role in the channel formation process.

Original languageEnglish (US)
Pages (from-to)141-144
Number of pages4
JournalEuropean Journal of Biochemistry
Issue number1
StatePublished - Apr 1991

ASJC Scopus subject areas

  • Biochemistry


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