Cell type of origin influences the molecular and functional properties of mouse induced pluripotent stem cells

Jose M. Polo, Susanna Liu, Maria Figueroa, Warakorn Kulalert, Sarah Eminli, Kah Yong Tan, Effie Apostolou, Matthias Stadtfeld, Yushan Li, Toshi Shioda, Sridaran Natesan, Amy J. Wagers, Ari Melnick, Todd Evans, Konrad Hochedlinger

Research output: Contribution to journalArticle

813 Citations (Scopus)

Abstract

Induced pluripotent stem cells (iPSCs) have been derived from various somatic cell populations through ectopic expression of defined factors. It remains unclear whether iPSCs generated from different cell types are molecularly and functionally similar. Here we show that iPSCs obtained from mouse fibroblasts, hematopoietic and myogenic cells exhibit distinct transcriptional and epigenetic patterns. Moreover, we demonstrate that cellular origin influences the in vitro differentiation potentials of iPSCs into embryoid bodies and different hematopoietic cell types. Notably, continuous passaging of iPSCs largely attenuates these differences. Our results suggest that early-passage iPSCs retain a transient epigenetic memory of their somatic cells of origin, which manifests as differential gene expression and altered differentiation capacity. These observations may influence ongoing attempts to use iPSCs for disease modeling and could also be exploited in potential therapeutic applications to enhance differentiation into desired cell lineages.

Original languageEnglish (US)
Pages (from-to)848-855
Number of pages8
JournalNature Biotechnology
Volume28
Issue number8
DOIs
StatePublished - Aug 1 2010
Externally publishedYes

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Induced Pluripotent Stem Cells
Stem cells
Epigenomics
Embryoid Bodies
Cell Lineage
Fibroblasts
Gene expression
Cells
Gene Expression
Data storage equipment
Population

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering
  • Molecular Medicine

Cite this

Cell type of origin influences the molecular and functional properties of mouse induced pluripotent stem cells. / Polo, Jose M.; Liu, Susanna; Figueroa, Maria; Kulalert, Warakorn; Eminli, Sarah; Tan, Kah Yong; Apostolou, Effie; Stadtfeld, Matthias; Li, Yushan; Shioda, Toshi; Natesan, Sridaran; Wagers, Amy J.; Melnick, Ari; Evans, Todd; Hochedlinger, Konrad.

In: Nature Biotechnology, Vol. 28, No. 8, 01.08.2010, p. 848-855.

Research output: Contribution to journalArticle

Polo, JM, Liu, S, Figueroa, M, Kulalert, W, Eminli, S, Tan, KY, Apostolou, E, Stadtfeld, M, Li, Y, Shioda, T, Natesan, S, Wagers, AJ, Melnick, A, Evans, T & Hochedlinger, K 2010, 'Cell type of origin influences the molecular and functional properties of mouse induced pluripotent stem cells', Nature Biotechnology, vol. 28, no. 8, pp. 848-855. https://doi.org/10.1038/nbt.1667
Polo, Jose M. ; Liu, Susanna ; Figueroa, Maria ; Kulalert, Warakorn ; Eminli, Sarah ; Tan, Kah Yong ; Apostolou, Effie ; Stadtfeld, Matthias ; Li, Yushan ; Shioda, Toshi ; Natesan, Sridaran ; Wagers, Amy J. ; Melnick, Ari ; Evans, Todd ; Hochedlinger, Konrad. / Cell type of origin influences the molecular and functional properties of mouse induced pluripotent stem cells. In: Nature Biotechnology. 2010 ; Vol. 28, No. 8. pp. 848-855.
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