Abstract
Induced pluripotent stem cells (iPSCs) have been derived from various somatic cell populations through ectopic expression of defined factors. It remains unclear whether iPSCs generated from different cell types are molecularly and functionally similar. Here we show that iPSCs obtained from mouse fibroblasts, hematopoietic and myogenic cells exhibit distinct transcriptional and epigenetic patterns. Moreover, we demonstrate that cellular origin influences the in vitro differentiation potentials of iPSCs into embryoid bodies and different hematopoietic cell types. Notably, continuous passaging of iPSCs largely attenuates these differences. Our results suggest that early-passage iPSCs retain a transient epigenetic memory of their somatic cells of origin, which manifests as differential gene expression and altered differentiation capacity. These observations may influence ongoing attempts to use iPSCs for disease modeling and could also be exploited in potential therapeutic applications to enhance differentiation into desired cell lineages.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 848-855 |
| Number of pages | 8 |
| Journal | Nature Biotechnology |
| Volume | 28 |
| Issue number | 8 |
| DOIs | |
| State | Published - Aug 1 2010 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
- Biomedical Engineering
- Molecular Medicine
Cite this
Cell type of origin influences the molecular and functional properties of mouse induced pluripotent stem cells. / Polo, Jose M.; Liu, Susanna; Figueroa, Maria; Kulalert, Warakorn; Eminli, Sarah; Tan, Kah Yong; Apostolou, Effie; Stadtfeld, Matthias; Li, Yushan; Shioda, Toshi; Natesan, Sridaran; Wagers, Amy J.; Melnick, Ari; Evans, Todd; Hochedlinger, Konrad.
In: Nature Biotechnology, Vol. 28, No. 8, 01.08.2010, p. 848-855.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cell type of origin influences the molecular and functional properties of mouse induced pluripotent stem cells
AU - Polo, Jose M.
AU - Liu, Susanna
AU - Figueroa, Maria
AU - Kulalert, Warakorn
AU - Eminli, Sarah
AU - Tan, Kah Yong
AU - Apostolou, Effie
AU - Stadtfeld, Matthias
AU - Li, Yushan
AU - Shioda, Toshi
AU - Natesan, Sridaran
AU - Wagers, Amy J.
AU - Melnick, Ari
AU - Evans, Todd
AU - Hochedlinger, Konrad
PY - 2010/8/1
Y1 - 2010/8/1
N2 - Induced pluripotent stem cells (iPSCs) have been derived from various somatic cell populations through ectopic expression of defined factors. It remains unclear whether iPSCs generated from different cell types are molecularly and functionally similar. Here we show that iPSCs obtained from mouse fibroblasts, hematopoietic and myogenic cells exhibit distinct transcriptional and epigenetic patterns. Moreover, we demonstrate that cellular origin influences the in vitro differentiation potentials of iPSCs into embryoid bodies and different hematopoietic cell types. Notably, continuous passaging of iPSCs largely attenuates these differences. Our results suggest that early-passage iPSCs retain a transient epigenetic memory of their somatic cells of origin, which manifests as differential gene expression and altered differentiation capacity. These observations may influence ongoing attempts to use iPSCs for disease modeling and could also be exploited in potential therapeutic applications to enhance differentiation into desired cell lineages.
AB - Induced pluripotent stem cells (iPSCs) have been derived from various somatic cell populations through ectopic expression of defined factors. It remains unclear whether iPSCs generated from different cell types are molecularly and functionally similar. Here we show that iPSCs obtained from mouse fibroblasts, hematopoietic and myogenic cells exhibit distinct transcriptional and epigenetic patterns. Moreover, we demonstrate that cellular origin influences the in vitro differentiation potentials of iPSCs into embryoid bodies and different hematopoietic cell types. Notably, continuous passaging of iPSCs largely attenuates these differences. Our results suggest that early-passage iPSCs retain a transient epigenetic memory of their somatic cells of origin, which manifests as differential gene expression and altered differentiation capacity. These observations may influence ongoing attempts to use iPSCs for disease modeling and could also be exploited in potential therapeutic applications to enhance differentiation into desired cell lineages.
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U2 - 10.1038/nbt.1667
DO - 10.1038/nbt.1667
M3 - Article
C2 - 20644536
AN - SCOPUS:78650752402
VL - 28
SP - 848
EP - 855
JO - Nature Biotechnology
JF - Nature Biotechnology
SN - 1087-0156
IS - 8
ER -