@article{404c1fe5eff748ab9c019fcab2c00920,
title = "Cell type of origin influences the molecular and functional properties of mouse induced pluripotent stem cells",
abstract = "Induced pluripotent stem cells (iPSCs) have been derived from various somatic cell populations through ectopic expression of defined factors. It remains unclear whether iPSCs generated from different cell types are molecularly and functionally similar. Here we show that iPSCs obtained from mouse fibroblasts, hematopoietic and myogenic cells exhibit distinct transcriptional and epigenetic patterns. Moreover, we demonstrate that cellular origin influences the in vitro differentiation potentials of iPSCs into embryoid bodies and different hematopoietic cell types. Notably, continuous passaging of iPSCs largely attenuates these differences. Our results suggest that early-passage iPSCs retain a transient epigenetic memory of their somatic cells of origin, which manifests as differential gene expression and altered differentiation capacity. These observations may influence ongoing attempts to use iPSCs for disease modeling and could also be exploited in potential therapeutic applications to enhance differentiation into desired cell lineages.",
author = "Polo, {Jose M.} and Susanna Liu and Figueroa, {Maria Eugenia} and Warakorn Kulalert and Sarah Eminli and Tan, {Kah Yong} and Effie Apostolou and Matthias Stadtfeld and Yushan Li and Toshi Shioda and Sridaran Natesan and Wagers, {Amy J.} and Ari Melnick and Todd Evans and Konrad Hochedlinger",
note = "Funding Information: We thank N. Maherali and R. Walsh for helpful suggestions and critical reading of the manuscript, B. Wittner for statistical advice, J. LaVecchio, G. Buruzula, K. Folz-Donahue and L. Prickett for expert cell sorting and K. Coser for technical assistance. J.M.P. was supported by an MGH ECOR fellowship, E.A. by a Jane Coffin Childs fellowship, M.S. by a Schering fellowship and K.Y.T. by the Agency of Science, Technology and Research Singapore. Support to A.M. was from the Lymphoma Society, SCOR no. 7132-08; to T.E. from National Institutes of Health (NIH) grant HL056182 and NYSTEM; to A.J.W. in part from the Burroughs Wellcome Fund, Harvard Stem Cell Institute, Peabody Foundation, and NIH 1 DP2 OD004345-01, and the Joslin Diabetes Center DERC (P30DK036836); to K.H. from Howard Hughes Medical Institute, the NIH Director{\textquoteright}s Innovator Award and the Harvard Stem Cell Institute. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.",
year = "2010",
month = aug,
doi = "10.1038/nbt.1667",
language = "English (US)",
volume = "28",
pages = "848--855",
journal = "Nature Biotechnology",
issn = "1087-0156",
publisher = "Nature Publishing Group",
number = "8",
}