TY - JOUR
T1 - Cell signaling by the type IV pili of pathogenic Neisseria
AU - Källström, Helena
AU - Islam, Md Shahidul
AU - Berggren, Per Olof
AU - Jonsson, Ann Beth
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1998/8/21
Y1 - 1998/8/21
N2 - Neisseria gonorrhoeae and Neisseria meningitidis are Gram-negative bacterial pathogens that infect human mucosal epithelia. Type IV pilus- mediated adherence of these bacteria is a crucial early event for establishment of infection. In this work, we show that the type IV pili transduce a signal into the eucaryotic host cell. Purified adherent pili, but not pill from a low binding mutant, trigger an increase in the cytosolic free calcium ([Ca2+](i)) in target epithelial cells, a signal known to control many cellular responses. The [Ca2+](i) increase was blocked by antibodies against CD46, a putative pilus receptor, suggesting a role for this protein in signal transduction. Pilus-mediated attachment was inhibited by depletion of host cell intracellular Ca2+ stores but not by removal of extracellular Ca2+. Further, kinase inhibition studies showed that pilus-mediated adherence is dependent on casein kinase II. In summary, these data reveal a novel function of the type IV pili, namely induction of signal transduction pathways in host cells.
AB - Neisseria gonorrhoeae and Neisseria meningitidis are Gram-negative bacterial pathogens that infect human mucosal epithelia. Type IV pilus- mediated adherence of these bacteria is a crucial early event for establishment of infection. In this work, we show that the type IV pili transduce a signal into the eucaryotic host cell. Purified adherent pili, but not pill from a low binding mutant, trigger an increase in the cytosolic free calcium ([Ca2+](i)) in target epithelial cells, a signal known to control many cellular responses. The [Ca2+](i) increase was blocked by antibodies against CD46, a putative pilus receptor, suggesting a role for this protein in signal transduction. Pilus-mediated attachment was inhibited by depletion of host cell intracellular Ca2+ stores but not by removal of extracellular Ca2+. Further, kinase inhibition studies showed that pilus-mediated adherence is dependent on casein kinase II. In summary, these data reveal a novel function of the type IV pili, namely induction of signal transduction pathways in host cells.
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U2 - 10.1074/jbc.273.34.21777
DO - 10.1074/jbc.273.34.21777
M3 - Article
C2 - 9705315
AN - SCOPUS:0032555594
VL - 273
SP - 21777
EP - 21782
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 34
ER -