Cell-permeable NM23 blocks the maintenance and progression of established pulmonary metastasis

Junghee Lim, Giyong Jang, Seeun Kang, Guewha Lee, Do Thi Thuy Nga, Do Thi Lan Phuong, Hyuncheol Kim, Wael El-Rifai, H. Earl Ruley, Daewoong Jo

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Occult metastases are a major cause of cancer mortality, even among patients undergoing curative resection. Therefore, practical strategies to target the growth and persistence of already established metastases would provide an important advance in cancer treatment. Here, we assessed the potential of protein therapy using a cell permeable NM23-H1 metastasis suppressor protein. Hydrophobic transduction domains developed from a screen of 1,500 signaling peptide sequences enhanced the uptake of the NM23 protein by cultured cells and systemic delivery to animal tissues. The cell-permeable (CP)-NM23 inhibited metastasis-associated phenotypes in tumor cell lines, blocked the establishment of lung metastases, and cleared already established pulmonary metastases, significantly prolonging the survival of tumor-bearing animals. Therefore, these results establish the potential use of cell-permeable metastasis suppressors as adjuvant therapy against disseminated cancers.

Original languageEnglish (US)
Pages (from-to)7216-7225
Number of pages10
JournalCancer Research
Volume71
Issue number23
DOIs
StatePublished - Dec 1 2011
Externally publishedYes

Fingerprint

Maintenance
Neoplasm Metastasis
Lung
Neoplasms
Tumor Suppressor Proteins
Tumor Cell Line
Cultured Cells
Proteins
Therapeutics
Phenotype
Peptides
Mortality
Growth

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Lim, J., Jang, G., Kang, S., Lee, G., Nga, D. T. T., Phuong, D. T. L., ... Jo, D. (2011). Cell-permeable NM23 blocks the maintenance and progression of established pulmonary metastasis. Cancer Research, 71(23), 7216-7225. https://doi.org/10.1158/0008-5472.CAN-11-2015

Cell-permeable NM23 blocks the maintenance and progression of established pulmonary metastasis. / Lim, Junghee; Jang, Giyong; Kang, Seeun; Lee, Guewha; Nga, Do Thi Thuy; Phuong, Do Thi Lan; Kim, Hyuncheol; El-Rifai, Wael; Ruley, H. Earl; Jo, Daewoong.

In: Cancer Research, Vol. 71, No. 23, 01.12.2011, p. 7216-7225.

Research output: Contribution to journalArticle

Lim, J, Jang, G, Kang, S, Lee, G, Nga, DTT, Phuong, DTL, Kim, H, El-Rifai, W, Ruley, HE & Jo, D 2011, 'Cell-permeable NM23 blocks the maintenance and progression of established pulmonary metastasis', Cancer Research, vol. 71, no. 23, pp. 7216-7225. https://doi.org/10.1158/0008-5472.CAN-11-2015
Lim, Junghee ; Jang, Giyong ; Kang, Seeun ; Lee, Guewha ; Nga, Do Thi Thuy ; Phuong, Do Thi Lan ; Kim, Hyuncheol ; El-Rifai, Wael ; Ruley, H. Earl ; Jo, Daewoong. / Cell-permeable NM23 blocks the maintenance and progression of established pulmonary metastasis. In: Cancer Research. 2011 ; Vol. 71, No. 23. pp. 7216-7225.
@article{a82a40b8a57e49a5a1a69a0f83697028,
title = "Cell-permeable NM23 blocks the maintenance and progression of established pulmonary metastasis",
abstract = "Occult metastases are a major cause of cancer mortality, even among patients undergoing curative resection. Therefore, practical strategies to target the growth and persistence of already established metastases would provide an important advance in cancer treatment. Here, we assessed the potential of protein therapy using a cell permeable NM23-H1 metastasis suppressor protein. Hydrophobic transduction domains developed from a screen of 1,500 signaling peptide sequences enhanced the uptake of the NM23 protein by cultured cells and systemic delivery to animal tissues. The cell-permeable (CP)-NM23 inhibited metastasis-associated phenotypes in tumor cell lines, blocked the establishment of lung metastases, and cleared already established pulmonary metastases, significantly prolonging the survival of tumor-bearing animals. Therefore, these results establish the potential use of cell-permeable metastasis suppressors as adjuvant therapy against disseminated cancers.",
author = "Junghee Lim and Giyong Jang and Seeun Kang and Guewha Lee and Nga, {Do Thi Thuy} and Phuong, {Do Thi Lan} and Hyuncheol Kim and Wael El-Rifai and Ruley, {H. Earl} and Daewoong Jo",
year = "2011",
month = "12",
day = "1",
doi = "10.1158/0008-5472.CAN-11-2015",
language = "English (US)",
volume = "71",
pages = "7216--7225",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "23",

}

TY - JOUR

T1 - Cell-permeable NM23 blocks the maintenance and progression of established pulmonary metastasis

AU - Lim, Junghee

AU - Jang, Giyong

AU - Kang, Seeun

AU - Lee, Guewha

AU - Nga, Do Thi Thuy

AU - Phuong, Do Thi Lan

AU - Kim, Hyuncheol

AU - El-Rifai, Wael

AU - Ruley, H. Earl

AU - Jo, Daewoong

PY - 2011/12/1

Y1 - 2011/12/1

N2 - Occult metastases are a major cause of cancer mortality, even among patients undergoing curative resection. Therefore, practical strategies to target the growth and persistence of already established metastases would provide an important advance in cancer treatment. Here, we assessed the potential of protein therapy using a cell permeable NM23-H1 metastasis suppressor protein. Hydrophobic transduction domains developed from a screen of 1,500 signaling peptide sequences enhanced the uptake of the NM23 protein by cultured cells and systemic delivery to animal tissues. The cell-permeable (CP)-NM23 inhibited metastasis-associated phenotypes in tumor cell lines, blocked the establishment of lung metastases, and cleared already established pulmonary metastases, significantly prolonging the survival of tumor-bearing animals. Therefore, these results establish the potential use of cell-permeable metastasis suppressors as adjuvant therapy against disseminated cancers.

AB - Occult metastases are a major cause of cancer mortality, even among patients undergoing curative resection. Therefore, practical strategies to target the growth and persistence of already established metastases would provide an important advance in cancer treatment. Here, we assessed the potential of protein therapy using a cell permeable NM23-H1 metastasis suppressor protein. Hydrophobic transduction domains developed from a screen of 1,500 signaling peptide sequences enhanced the uptake of the NM23 protein by cultured cells and systemic delivery to animal tissues. The cell-permeable (CP)-NM23 inhibited metastasis-associated phenotypes in tumor cell lines, blocked the establishment of lung metastases, and cleared already established pulmonary metastases, significantly prolonging the survival of tumor-bearing animals. Therefore, these results establish the potential use of cell-permeable metastasis suppressors as adjuvant therapy against disseminated cancers.

UR - http://www.scopus.com/inward/record.url?scp=82655189970&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=82655189970&partnerID=8YFLogxK

U2 - 10.1158/0008-5472.CAN-11-2015

DO - 10.1158/0008-5472.CAN-11-2015

M3 - Article

C2 - 21987726

AN - SCOPUS:82655189970

VL - 71

SP - 7216

EP - 7225

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 23

ER -