Cell-mediated lympholysis responses againt autologous cells modified with haptenic sulfhydryl reagents. II. Analysis of the genetic control and H-2-restricted pattern of cytotoxic responses to sulfhydryl and amino reactive reagents

Robert B Levy, P. A. Henkart, G. M. Shearer

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

N-iodoacetyl-N'-(5-sulfonic-1-naphthy)ethylene-diamine modifed autologous cells are shown to induce respective high and low AED-self CTL responses by spleen cells from H-2(b) and H-2(k) mice. The lysis mediated by effector populations from both haplotypes was highly H-2 restricted. Cytotoxic responses induced by a 2nd sulfhydryl- (SH) specific reagent, dithionitrobenzoic acid (DTNB), were shown not to be cross-reactive at the effector cell level with AED-self but exhibited the same high and low CTL response patterns as those induced against AED-self. These Ir-effects reported for the 2 SH reactive compounds are discussed in relation to the opposite Ir-patterns demonstrated against a number of amino (NH2) reactive compounds. The difference of self-recognition sites is suggested to account for the contrasting results observed in the response patterns induced by SH- and NH2-reactive compounds. The AED-self CTL response was found to exhibit K(k)-self over D(b)-self Ir regulation. Thus, this latter type of genetic control appears to function independently of whether the hapten is an SH- or NH2-reactive compound.

Original languageEnglish
Pages (from-to)529-534
Number of pages6
JournalJournal of Immunology
Volume127
Issue number2
StatePublished - Jan 1 1981
Externally publishedYes

Fingerprint

Sulfhydryl Reagents
Dithionitrobenzoic Acid
Diamines
Haptens
Sulfhydryl Compounds
Haplotypes
Spleen
Population

ASJC Scopus subject areas

  • Immunology

Cite this

@article{4c727d3adbcf497fbdc0639681d274cd,
title = "Cell-mediated lympholysis responses againt autologous cells modified with haptenic sulfhydryl reagents. II. Analysis of the genetic control and H-2-restricted pattern of cytotoxic responses to sulfhydryl and amino reactive reagents",
abstract = "N-iodoacetyl-N'-(5-sulfonic-1-naphthy)ethylene-diamine modifed autologous cells are shown to induce respective high and low AED-self CTL responses by spleen cells from H-2(b) and H-2(k) mice. The lysis mediated by effector populations from both haplotypes was highly H-2 restricted. Cytotoxic responses induced by a 2nd sulfhydryl- (SH) specific reagent, dithionitrobenzoic acid (DTNB), were shown not to be cross-reactive at the effector cell level with AED-self but exhibited the same high and low CTL response patterns as those induced against AED-self. These Ir-effects reported for the 2 SH reactive compounds are discussed in relation to the opposite Ir-patterns demonstrated against a number of amino (NH2) reactive compounds. The difference of self-recognition sites is suggested to account for the contrasting results observed in the response patterns induced by SH- and NH2-reactive compounds. The AED-self CTL response was found to exhibit K(k)-self over D(b)-self Ir regulation. Thus, this latter type of genetic control appears to function independently of whether the hapten is an SH- or NH2-reactive compound.",
author = "Levy, {Robert B} and Henkart, {P. A.} and Shearer, {G. M.}",
year = "1981",
month = "1",
day = "1",
language = "English",
volume = "127",
pages = "529--534",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "2",

}

TY - JOUR

T1 - Cell-mediated lympholysis responses againt autologous cells modified with haptenic sulfhydryl reagents. II. Analysis of the genetic control and H-2-restricted pattern of cytotoxic responses to sulfhydryl and amino reactive reagents

AU - Levy, Robert B

AU - Henkart, P. A.

AU - Shearer, G. M.

PY - 1981/1/1

Y1 - 1981/1/1

N2 - N-iodoacetyl-N'-(5-sulfonic-1-naphthy)ethylene-diamine modifed autologous cells are shown to induce respective high and low AED-self CTL responses by spleen cells from H-2(b) and H-2(k) mice. The lysis mediated by effector populations from both haplotypes was highly H-2 restricted. Cytotoxic responses induced by a 2nd sulfhydryl- (SH) specific reagent, dithionitrobenzoic acid (DTNB), were shown not to be cross-reactive at the effector cell level with AED-self but exhibited the same high and low CTL response patterns as those induced against AED-self. These Ir-effects reported for the 2 SH reactive compounds are discussed in relation to the opposite Ir-patterns demonstrated against a number of amino (NH2) reactive compounds. The difference of self-recognition sites is suggested to account for the contrasting results observed in the response patterns induced by SH- and NH2-reactive compounds. The AED-self CTL response was found to exhibit K(k)-self over D(b)-self Ir regulation. Thus, this latter type of genetic control appears to function independently of whether the hapten is an SH- or NH2-reactive compound.

AB - N-iodoacetyl-N'-(5-sulfonic-1-naphthy)ethylene-diamine modifed autologous cells are shown to induce respective high and low AED-self CTL responses by spleen cells from H-2(b) and H-2(k) mice. The lysis mediated by effector populations from both haplotypes was highly H-2 restricted. Cytotoxic responses induced by a 2nd sulfhydryl- (SH) specific reagent, dithionitrobenzoic acid (DTNB), were shown not to be cross-reactive at the effector cell level with AED-self but exhibited the same high and low CTL response patterns as those induced against AED-self. These Ir-effects reported for the 2 SH reactive compounds are discussed in relation to the opposite Ir-patterns demonstrated against a number of amino (NH2) reactive compounds. The difference of self-recognition sites is suggested to account for the contrasting results observed in the response patterns induced by SH- and NH2-reactive compounds. The AED-self CTL response was found to exhibit K(k)-self over D(b)-self Ir regulation. Thus, this latter type of genetic control appears to function independently of whether the hapten is an SH- or NH2-reactive compound.

UR - http://www.scopus.com/inward/record.url?scp=0019503497&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019503497&partnerID=8YFLogxK

M3 - Article

C2 - 6972968

AN - SCOPUS:0019503497

VL - 127

SP - 529

EP - 534

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 2

ER -