Cell kinetics in vivo and in vitro for C3H/He spontaneous mammary tumors

Paul G. Braunschweiger, Lawrence Poulakos, Lewis M. Schiffer

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Cell kinetics in spontaneous C3H/HeJ mammary tumors of retired-breeder mice was studied by in vivo and in vitro techniques. The [3H]TdR labeling index (LI), the DNA synthesis time (T(S)), and the primer-dependent DNA polymerase assay LI [an in vitro estimate of tumor growth fraction (GF)] were compared to similar measurements made in vivo. These measurements as well as the calculated cell kinetic parameters derived from these data were not different in in vivo and in vitro studies. Furthermore, the cell kinetic parameters in tumors classified histologically as type A or type B mammary tumors were also similar. Although considerable variation in volume doubling times (Td's), [3H]TdR LI's, potential doubling times, cell cycle times (Tc's), and cell loss was found, T(S)'s were similar in all mammary tumors. No correlation between tumor volume or tumor weight and cell kinetic parameters was seen. However, the most slowly growing tumors (i.e., tumors with the longest Td's) tended to have the lowest [3H]TdR LI's, the longest Tc's, and the highest cell loss factors. No correlation was found between the GF and Td. However, tumors with the most rapidly proliferating cell populations tended to have the highest GF's.

Original languageEnglish (US)
Pages (from-to)1197-1204
Number of pages8
JournalJournal of the National Cancer Institute
Volume59
Issue number4
DOIs
StatePublished - Oct 1977

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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