Cell kinetic alterations in murine mammary tumors following fasting and refeeding

James J. Stragand, Paul G Braunschweiger, Agnese A. Pollice, Lewis M. Schiffer

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

In the present study, nutritional deprivation was shown to markedly alter the growth and cell kinetics of two widely different experimental tumors, the T-1699 transplantable mammary tumor (TMT) and the C3H HeJ spontaneous mammary tumor (SMT). In vitro techniques were used to determine the thymidine labeling index ([3H]TdR LI), the tumor growth fraction, by the primer available DNA polymerase assay (PDP), and the DNA synthesis time (TS) following fasting and refeeding. In both systems, fasting resulted in suppression of cell proliferation and significant tumor regression. Refeeding re-established the prefasting growth rates and cell kinetic profiles. The kinetic response to fasting in the TMT involved a generalized lengthening of the cell cycle (TC), while the timing and magnitude of the response to refeeding appear to be dose dependent. In contrast, the kinetic data from the SMT during fasting was consistent with the presence of a cell cycle block in G1. Removal of this block by refeeding was prompt and was characterized by tumor cell synchronization and recruitment of non-cycling cells.

Original languageEnglish
Pages (from-to)281-286
Number of pages6
JournalEuropean Journal of Cancer (1965)
Volume15
Issue number3
DOIs
StatePublished - Jan 1 1979
Externally publishedYes

Fingerprint

Fasting
Breast Neoplasms
Neoplasms
Cell Cycle
Growth
DNA-Directed DNA Polymerase
Thymidine
Cell Proliferation
DNA

ASJC Scopus subject areas

  • Oncology

Cite this

Cell kinetic alterations in murine mammary tumors following fasting and refeeding. / Stragand, James J.; Braunschweiger, Paul G; Pollice, Agnese A.; Schiffer, Lewis M.

In: European Journal of Cancer (1965), Vol. 15, No. 3, 01.01.1979, p. 281-286.

Research output: Contribution to journalArticle

Stragand, James J. ; Braunschweiger, Paul G ; Pollice, Agnese A. ; Schiffer, Lewis M. / Cell kinetic alterations in murine mammary tumors following fasting and refeeding. In: European Journal of Cancer (1965). 1979 ; Vol. 15, No. 3. pp. 281-286.
@article{9ab0b9cfa3864ca99d8ab1ba946e0268,
title = "Cell kinetic alterations in murine mammary tumors following fasting and refeeding",
abstract = "In the present study, nutritional deprivation was shown to markedly alter the growth and cell kinetics of two widely different experimental tumors, the T-1699 transplantable mammary tumor (TMT) and the C3H HeJ spontaneous mammary tumor (SMT). In vitro techniques were used to determine the thymidine labeling index ([3H]TdR LI), the tumor growth fraction, by the primer available DNA polymerase assay (PDP), and the DNA synthesis time (TS) following fasting and refeeding. In both systems, fasting resulted in suppression of cell proliferation and significant tumor regression. Refeeding re-established the prefasting growth rates and cell kinetic profiles. The kinetic response to fasting in the TMT involved a generalized lengthening of the cell cycle (TC), while the timing and magnitude of the response to refeeding appear to be dose dependent. In contrast, the kinetic data from the SMT during fasting was consistent with the presence of a cell cycle block in G1. Removal of this block by refeeding was prompt and was characterized by tumor cell synchronization and recruitment of non-cycling cells.",
author = "Stragand, {James J.} and Braunschweiger, {Paul G} and Pollice, {Agnese A.} and Schiffer, {Lewis M.}",
year = "1979",
month = "1",
day = "1",
doi = "10.1016/0014-2964(79)90038-0",
language = "English",
volume = "15",
pages = "281--286",
journal = "European journal of cancer",
issn = "0014-2964",
publisher = "Pergamon Press Ltd.",
number = "3",

}

TY - JOUR

T1 - Cell kinetic alterations in murine mammary tumors following fasting and refeeding

AU - Stragand, James J.

AU - Braunschweiger, Paul G

AU - Pollice, Agnese A.

AU - Schiffer, Lewis M.

PY - 1979/1/1

Y1 - 1979/1/1

N2 - In the present study, nutritional deprivation was shown to markedly alter the growth and cell kinetics of two widely different experimental tumors, the T-1699 transplantable mammary tumor (TMT) and the C3H HeJ spontaneous mammary tumor (SMT). In vitro techniques were used to determine the thymidine labeling index ([3H]TdR LI), the tumor growth fraction, by the primer available DNA polymerase assay (PDP), and the DNA synthesis time (TS) following fasting and refeeding. In both systems, fasting resulted in suppression of cell proliferation and significant tumor regression. Refeeding re-established the prefasting growth rates and cell kinetic profiles. The kinetic response to fasting in the TMT involved a generalized lengthening of the cell cycle (TC), while the timing and magnitude of the response to refeeding appear to be dose dependent. In contrast, the kinetic data from the SMT during fasting was consistent with the presence of a cell cycle block in G1. Removal of this block by refeeding was prompt and was characterized by tumor cell synchronization and recruitment of non-cycling cells.

AB - In the present study, nutritional deprivation was shown to markedly alter the growth and cell kinetics of two widely different experimental tumors, the T-1699 transplantable mammary tumor (TMT) and the C3H HeJ spontaneous mammary tumor (SMT). In vitro techniques were used to determine the thymidine labeling index ([3H]TdR LI), the tumor growth fraction, by the primer available DNA polymerase assay (PDP), and the DNA synthesis time (TS) following fasting and refeeding. In both systems, fasting resulted in suppression of cell proliferation and significant tumor regression. Refeeding re-established the prefasting growth rates and cell kinetic profiles. The kinetic response to fasting in the TMT involved a generalized lengthening of the cell cycle (TC), while the timing and magnitude of the response to refeeding appear to be dose dependent. In contrast, the kinetic data from the SMT during fasting was consistent with the presence of a cell cycle block in G1. Removal of this block by refeeding was prompt and was characterized by tumor cell synchronization and recruitment of non-cycling cells.

UR - http://www.scopus.com/inward/record.url?scp=0018424558&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018424558&partnerID=8YFLogxK

U2 - 10.1016/0014-2964(79)90038-0

DO - 10.1016/0014-2964(79)90038-0

M3 - Article

VL - 15

SP - 281

EP - 286

JO - European journal of cancer

JF - European journal of cancer

SN - 0014-2964

IS - 3

ER -