Cell free translation of simian virus 40 early messenger RNA coding for viral T antigen

C. Prives, Eli Gilboa, M. Revel, E. Winocour

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Simian virus 40 (SV40) mRNA was isolated by hybridization of cytoplasmic RNA, from SV40-infected BS-C-1 monkey cells early in lytic infection, to SV40 DNA immobilized on Sepharose. The early viral mRNA, when added to a wheat-germ translation system, directed the synthesis of a unique class of products including a 90,000 molecular weight (Mr) polypeptide. It was found that this 90,000 Mr product as well as a prominent 17,000 Mr polypeptide could be specifically immunoprecipitated with hamster antiserum to SV40 T-antigen, but not with hamster control serum. Similar immunoprecipitation of extracts of SV40-infected cells with hamster anti-T serum yielded 90,000 Mr and 17,000 Mr polypeptides; these polypeptides were not found in immunoprecipitates of uninfected cell extracts. SV40 cRNA, prepared by asymmetric transcription of plaque-purified SV40 DNA, directed the cell-free synthesis of several products, including a 70,000 Mr polypeptide that could be specifically immunoprecipitated with anti-T serum. However, no T-antigen-related polypeptide was found in infected cells that corresponded in size to the major immunoprecipitated cRNA product.

Original languageEnglish
Pages (from-to)457-461
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume74
Issue number2
StatePublished - Jan 1 1977
Externally publishedYes

Fingerprint

Simian virus 40
Viral Tumor Antigens
Messenger RNA
Peptides
Cricetinae
Complementary RNA
Serum
Immobilized Nucleic Acids
RNA Viruses
Cell Extracts
Immunoprecipitation
Sepharose
Triticum
Haplorhini
Immune Sera
Molecular Weight
DNA
Infection

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Cell free translation of simian virus 40 early messenger RNA coding for viral T antigen. / Prives, C.; Gilboa, Eli; Revel, M.; Winocour, E.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 74, No. 2, 01.01.1977, p. 457-461.

Research output: Contribution to journalArticle

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