Abstract
In an attempt to define more clearly a possible role for polyamine synthesis on the growth and cell cycle progression, the effects of methylglyoxal-bis (guanylhydrazone i.e. MGBG, an inhibitor of polyamine synthesis on the proliferation of mouse leukaemia L1210 cells in culture have been studied. Treatment of cells with MGBG resulted in a time and dosage dependent growth inhibition ultimately resulting in cytotoxicity. There was an early accumulation of G1 cell population with a concomitant decrease in labelling index indicating a decrease in S phase cell population. Following prolonged treatment and at higher dose levels, an accumulation of cells also occurred in G2 phase with a parallel decrease in mitotic index of cells, indicating a blockade in progression of cells from G2 to M phase of cell cycle. A sudden early decrease (at 4 h, approximately 70 per cent of control) and a gradual decline later on (up to 15 per cent of control) in intracellular spermidine pools suggests a possible role of polyamines in cell cycle traverse. A possible explanation for these effects of MGBG has been offered.
| Original language | English |
|---|---|
| Pages (from-to) | 777-792 |
| Number of pages | 16 |
| Journal | Indian Journal of Medical Research |
| Volume | 70 |
| Issue number | 5 |
| State | Published - Dec 1 1979 |
| Externally published | Yes |
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Cell cycle phase specificity of action of methyl/glyoxal-bis (guanylhydrazone) and its relationship to polyamine biosynthesis on mouse leukaemia L1210 cells in culture. / Pathak, S. N.; Dave, C.; Krishan, A.
In: Indian Journal of Medical Research, Vol. 70, No. 5, 01.12.1979, p. 777-792.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cell cycle phase specificity of action of methyl/glyoxal-bis (guanylhydrazone) and its relationship to polyamine biosynthesis on mouse leukaemia L1210 cells in culture
AU - Pathak, S. N.
AU - Dave, C.
AU - Krishan, A.
PY - 1979/12/1
Y1 - 1979/12/1
N2 - In an attempt to define more clearly a possible role for polyamine synthesis on the growth and cell cycle progression, the effects of methylglyoxal-bis (guanylhydrazone i.e. MGBG, an inhibitor of polyamine synthesis on the proliferation of mouse leukaemia L1210 cells in culture have been studied. Treatment of cells with MGBG resulted in a time and dosage dependent growth inhibition ultimately resulting in cytotoxicity. There was an early accumulation of G1 cell population with a concomitant decrease in labelling index indicating a decrease in S phase cell population. Following prolonged treatment and at higher dose levels, an accumulation of cells also occurred in G2 phase with a parallel decrease in mitotic index of cells, indicating a blockade in progression of cells from G2 to M phase of cell cycle. A sudden early decrease (at 4 h, approximately 70 per cent of control) and a gradual decline later on (up to 15 per cent of control) in intracellular spermidine pools suggests a possible role of polyamines in cell cycle traverse. A possible explanation for these effects of MGBG has been offered.
AB - In an attempt to define more clearly a possible role for polyamine synthesis on the growth and cell cycle progression, the effects of methylglyoxal-bis (guanylhydrazone i.e. MGBG, an inhibitor of polyamine synthesis on the proliferation of mouse leukaemia L1210 cells in culture have been studied. Treatment of cells with MGBG resulted in a time and dosage dependent growth inhibition ultimately resulting in cytotoxicity. There was an early accumulation of G1 cell population with a concomitant decrease in labelling index indicating a decrease in S phase cell population. Following prolonged treatment and at higher dose levels, an accumulation of cells also occurred in G2 phase with a parallel decrease in mitotic index of cells, indicating a blockade in progression of cells from G2 to M phase of cell cycle. A sudden early decrease (at 4 h, approximately 70 per cent of control) and a gradual decline later on (up to 15 per cent of control) in intracellular spermidine pools suggests a possible role of polyamines in cell cycle traverse. A possible explanation for these effects of MGBG has been offered.
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M3 - Article
AN - SCOPUS:0018598638
VL - 70
SP - 777
EP - 792
JO - Indian Journal of Medical Research
JF - Indian Journal of Medical Research
SN - 0019-5340
IS - 5
ER -