Cell cycle control of DNA replication by a homologue from human cells of the p34cdc2 protein kinase

Gennaro D'Urso, Robert L. Marraccino, Daniel R. Marshak, James M. Roberts

Research output: Contribution to journalArticlepeer-review

198 Scopus citations

Abstract

The regulation of DNA replication during the eukaryotic cell cycle was studied in a system where cell free replication of simian virus 40 (SV40) DNA was used as a model for chromosome replication. A factor, RF-S, was partially purified from human S phase cells based on its ability to activate DNA replication in extracts from G1 cells. RF-S contained a human homologue of the Schizosaccharomyces pombe p34cdc2 kinase, and this kinase was necessary for RF-S activity. The limiting step in activation of the p34 kinase at the G1 to S transition may be its association with a cyclin since addition of cyclin A to a G1 extract was sufficient to start DNA replication. These observations suggest that the role of p34cdc2 in controlling the start of DNA synthesis has been conserved in evolution.

Original languageEnglish (US)
Pages (from-to)780-791
Number of pages12
JournalScience
Volume250
Issue number4982
StatePublished - Jan 1 1990
Externally publishedYes

ASJC Scopus subject areas

  • General

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