Cell cycle arrest and inhibition of tumor cell proliferation by the p16INK4 gene mediated by an adenovirus vector

Xiaomei Jin, Dao Nguyen, Wei Wei Zhang, Athanassios P. Kyritsis, Jack A. Roth

Research output: Contribution to journalArticle

173 Citations (Scopus)

Abstract

The p16INK4 (MTS1) gene has many features of a tumor suppressor gene. It maps to 9p21, a region of frequent loss of heterozygosity in a variety of tumor types. It encodes an inhibitor of cyclin-dependent kinase 4, and its homozygous deletion is common in tumor-derived cell lines. To examine its tumor suppressive function and its potential in cancer gene replacement therapy, wild-type p16INK4 was expressed in an adenovirus-derived gene delivery system and introduced into lung cancer cell lines that do not express p16lNK4. Expression of the introduced p16INK4 blocked tumor cell entry into S phase of the cell cycle and inhibited tumor proliferation both in vitro and in vivo. These observations strongly support that p16INK4 is a tumor suppressor gene and is a candidate for cancer gene replacement therapy.

Original languageEnglish
Pages (from-to)3250-3253
Number of pages4
JournalCancer Research
Volume55
Issue number15
StatePublished - Dec 1 1995
Externally publishedYes

Fingerprint

p16 Genes
Cell Cycle Checkpoints
Adenoviridae
Cell Proliferation
Neoplasm Genes
Tumor Suppressor Genes
Genetic Therapy
Neoplasms
Cyclin-Dependent Kinase 4
Gene Transfer Techniques
Loss of Heterozygosity
Tumor Cell Line
S Phase
Lung Neoplasms
Cell Cycle
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Cell cycle arrest and inhibition of tumor cell proliferation by the p16INK4 gene mediated by an adenovirus vector. / Jin, Xiaomei; Nguyen, Dao; Zhang, Wei Wei; Kyritsis, Athanassios P.; Roth, Jack A.

In: Cancer Research, Vol. 55, No. 15, 01.12.1995, p. 3250-3253.

Research output: Contribution to journalArticle

Jin, Xiaomei ; Nguyen, Dao ; Zhang, Wei Wei ; Kyritsis, Athanassios P. ; Roth, Jack A. / Cell cycle arrest and inhibition of tumor cell proliferation by the p16INK4 gene mediated by an adenovirus vector. In: Cancer Research. 1995 ; Vol. 55, No. 15. pp. 3250-3253.
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