Cell Cycle Arrest and Inhibition of Tumor Cell Proliferation by the p16INK4 Gene Mediated by an Adenovirus Vector

Xiaomei Jin, Dao Nguyen, Zhang Wei-Wei, Athanassios P. Kyritsis, Jack A. Roth

Research output: Contribution to journalArticlepeer-review

173 Scopus citations

Abstract

The p16INK4 (MTS1) gene has many features of a tumor suppressor gene. It maps to 9p21, a region of frequent loss of heterozygosity in a variety of tumor types. It encodes an inhibitor of cyclin-dependent kinase 4, and its homozygous deletion is common in tumor-derived cell lines. To examine its tumor suppressive function and its potential in cancer gene replacement therapy, wild-type p16INK4 was expressed in an adenovirus-derived gene delivery system and introduced into lung cancer cell lines that do not express p16lNK4. Expression of the introduced p16INK4 blocked tumor cell entry into S phase of the cell cycle and inhibited tumor proliferation both in vitro and in vivo. These observations strongly support that p16INK4 is a tumor suppressor gene and is a candidate for cancer gene replacement therapy.

Original languageEnglish (US)
Pages (from-to)3250-3253
Number of pages4
JournalCancer Research
Volume55
Issue number15
StatePublished - Aug 1 1995
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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