Cell cycle analysis by flow cytometry of non-exposed, sun-exposed, and tretinoin-treated skin

S. T. Nedorost, W. F. Bergfeld, R. Valenzuela, J. Gultart, M. G. Fleming

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3 Scopus citations


The percentage of keratinocytes in the proliferative phase of the cell cycle (S + G2 + M) was measured by DNA flow cytometry in sun-exposed, non-exposed, and tretinoin-treated skin. Before tretinoin treatment, the percentage of keratinocytes actively cycling was higher in sun-exposed than in non-sun-exposed skin (p = .002) and was correlated with clinically assessed photodamage (p = .007). Subsequently, tretinoin-treated sun-exposed skin was compared to the pre-treatment sun-exposed skin. Overall, there was no statistically significant change. However, there was a trend toward a decrease in the percentage of keratinocytes in the S + G2 + M phases immediately after four months of tretinoin use that was limited to the most severely damaged patients. This effect was no longer evident two months after discontinuing treatment. This is the first study, to our knowledge, utilizing flow cytometry to investigate the effects of tretinoin in patients with varying degress of photodamage.

Original languageEnglish
Pages (from-to)371-375
Number of pages5
JournalJournal of Cutaneous Pathology
Issue number5
StatePublished - Nov 11 1992
Externally publishedYes


ASJC Scopus subject areas

  • Dermatology
  • Pathology and Forensic Medicine

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