Abstract
Objective: We examined the effect of an HIV-1-specific immune-based therapy on cell-associated HIV-1 DNA and RNA. Design: Five HIV-1-infected subjects receiving HIV-1 immunogen plus HAART were compared with three HIV-1-infected subjects who received incomplete Freund's adjuvant (IFA) plus HAART. Methods: Cell-associated HIV-1 RNA or DNA in lymphocytes and monocytes was determined using a dual immunophenotyping/in situ hybridization assay with or without in situ PCR amplification. Results: Cell-associated HIV-1 RNA in CD4 cells correlated with plasma RNA overall. CD4, HIV-1 gag-pol messenger (m)RNA+ cells decreased in the immunogen plus HAART group compared with the IFA plus HAART group. Decreases in HIV-1 DNA+ CD4 cells were observed in the immunogen plus HAART compared with the IFA plus HAART group. Decreases in HIV-1 gag-pol mRNA+ monocytes were observed in the immunogen plus HAART group compared with the IFA plus HAART group. Consistent with the findings in CD4 cells, decreases in HIV-1 DNA+ monocytes were observed in the immunogen plus HAART group compared with the IFA plus HAART group. Conclusions: These preliminary observations support the rationale for examining the combination of immune-based therapies and antiretroviral drugs for effective HIV-1 control.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1607-1611 |
| Number of pages | 5 |
| Journal | AIDS |
| Volume | 13 |
| Issue number | 13 |
| DOIs | |
| State | Published - Dec 1 1999 |
| Externally published | Yes |
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Keywords
- CD14
- CD4
- Flow cytometry
- HIV-1 viral load
- In situ hybridization
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
Cell-associated HIV-1 messenger RNA and DNA in T-helper cell and monocytes in asymptomatic HIV-1-infected subjects on HAART plus an inactivated HIV-1 immunogen. / Patterson, Bruce K.; Carlo, Dennis J.; Kaplan, Mark H.; Marecki, Maria; Pahwa, Savita G; Moss, Ronald B.
In: AIDS, Vol. 13, No. 13, 01.12.1999, p. 1607-1611.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cell-associated HIV-1 messenger RNA and DNA in T-helper cell and monocytes in asymptomatic HIV-1-infected subjects on HAART plus an inactivated HIV-1 immunogen
AU - Patterson, Bruce K.
AU - Carlo, Dennis J.
AU - Kaplan, Mark H.
AU - Marecki, Maria
AU - Pahwa, Savita G
AU - Moss, Ronald B.
PY - 1999/12/1
Y1 - 1999/12/1
N2 - Objective: We examined the effect of an HIV-1-specific immune-based therapy on cell-associated HIV-1 DNA and RNA. Design: Five HIV-1-infected subjects receiving HIV-1 immunogen plus HAART were compared with three HIV-1-infected subjects who received incomplete Freund's adjuvant (IFA) plus HAART. Methods: Cell-associated HIV-1 RNA or DNA in lymphocytes and monocytes was determined using a dual immunophenotyping/in situ hybridization assay with or without in situ PCR amplification. Results: Cell-associated HIV-1 RNA in CD4 cells correlated with plasma RNA overall. CD4, HIV-1 gag-pol messenger (m)RNA+ cells decreased in the immunogen plus HAART group compared with the IFA plus HAART group. Decreases in HIV-1 DNA+ CD4 cells were observed in the immunogen plus HAART compared with the IFA plus HAART group. Decreases in HIV-1 gag-pol mRNA+ monocytes were observed in the immunogen plus HAART group compared with the IFA plus HAART group. Consistent with the findings in CD4 cells, decreases in HIV-1 DNA+ monocytes were observed in the immunogen plus HAART group compared with the IFA plus HAART group. Conclusions: These preliminary observations support the rationale for examining the combination of immune-based therapies and antiretroviral drugs for effective HIV-1 control.
AB - Objective: We examined the effect of an HIV-1-specific immune-based therapy on cell-associated HIV-1 DNA and RNA. Design: Five HIV-1-infected subjects receiving HIV-1 immunogen plus HAART were compared with three HIV-1-infected subjects who received incomplete Freund's adjuvant (IFA) plus HAART. Methods: Cell-associated HIV-1 RNA or DNA in lymphocytes and monocytes was determined using a dual immunophenotyping/in situ hybridization assay with or without in situ PCR amplification. Results: Cell-associated HIV-1 RNA in CD4 cells correlated with plasma RNA overall. CD4, HIV-1 gag-pol messenger (m)RNA+ cells decreased in the immunogen plus HAART group compared with the IFA plus HAART group. Decreases in HIV-1 DNA+ CD4 cells were observed in the immunogen plus HAART compared with the IFA plus HAART group. Decreases in HIV-1 gag-pol mRNA+ monocytes were observed in the immunogen plus HAART group compared with the IFA plus HAART group. Consistent with the findings in CD4 cells, decreases in HIV-1 DNA+ monocytes were observed in the immunogen plus HAART group compared with the IFA plus HAART group. Conclusions: These preliminary observations support the rationale for examining the combination of immune-based therapies and antiretroviral drugs for effective HIV-1 control.
KW - CD14
KW - CD4
KW - Flow cytometry
KW - HIV-1 viral load
KW - In situ hybridization
UR - http://www.scopus.com/inward/record.url?scp=0033385794&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033385794&partnerID=8YFLogxK
U2 - 10.1097/00002030-199909100-00002
DO - 10.1097/00002030-199909100-00002
M3 - Article
C2 - 10509560
AN - SCOPUS:0033385794
VL - 13
SP - 1607
EP - 1611
JO - AIDS
JF - AIDS
SN - 0269-9370
IS - 13
ER -