CDS negative "small agranular lymphocytes" ARE natural killer cells

Luca A Inverardi, Jean C. Witson, Susan A. Fuad, Robin T. Winkler-Pickett, John R. Ortaldo, Fritz H. Bach

Research output: Contribution to journalArticle

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Abstract

We describe here that CD3-, CD16+ and/or CD56+ small lymphocytes, in a highly reproducible fashion, mediate a significant level of K562 killing that is, on a "per cell" basis, comparable to the cytolytic activity of CD3- LGL. The CD3- small lymphocytes appeared to have no granules based on light and electron microscopy and lack of right-angle scatter on the FACS; we thus refer to them as small "agranular" lymphocytes (SAL). The lytic activity against K562 is inhibited by treatment with either L-leucine methyl ester or EGTA, which are reported to effect granule-dependent killing. We suggest that the SAL have lytic molecules in their cytoplasm (which are sensitive to these treatments) but that these molecules are not organized into discrete granules as found in LGL. The CD3- SAL are phenotypically very similar to LGL and both SAL and LGL mediated equal and reproducible antibody-dependent cell-mediated cytotoxicity. These observations force redefinition of the concept of NK cells to include both CD3- LGL and CD3- SAL.

Original languageEnglish
Pages (from-to)4048-4052
Number of pages5
JournalJournal of Immunology
Volume146
Issue number11
StatePublished - Jun 1 1991
Externally publishedYes

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Natural Killer Cells
Lymphocytes
Antibody-Dependent Cell Cytotoxicity
Egtazic Acid
Leucine
Electron Microscopy
Cytoplasm
Light

ASJC Scopus subject areas

  • Immunology

Cite this

Inverardi, L. A., Witson, J. C., Fuad, S. A., Winkler-Pickett, R. T., Ortaldo, J. R., & Bach, F. H. (1991). CDS negative "small agranular lymphocytes" ARE natural killer cells. Journal of Immunology, 146(11), 4048-4052.

CDS negative "small agranular lymphocytes" ARE natural killer cells. / Inverardi, Luca A; Witson, Jean C.; Fuad, Susan A.; Winkler-Pickett, Robin T.; Ortaldo, John R.; Bach, Fritz H.

In: Journal of Immunology, Vol. 146, No. 11, 01.06.1991, p. 4048-4052.

Research output: Contribution to journalArticle

Inverardi, LA, Witson, JC, Fuad, SA, Winkler-Pickett, RT, Ortaldo, JR & Bach, FH 1991, 'CDS negative "small agranular lymphocytes" ARE natural killer cells', Journal of Immunology, vol. 146, no. 11, pp. 4048-4052.
Inverardi LA, Witson JC, Fuad SA, Winkler-Pickett RT, Ortaldo JR, Bach FH. CDS negative "small agranular lymphocytes" ARE natural killer cells. Journal of Immunology. 1991 Jun 1;146(11):4048-4052.
Inverardi, Luca A ; Witson, Jean C. ; Fuad, Susan A. ; Winkler-Pickett, Robin T. ; Ortaldo, John R. ; Bach, Fritz H. / CDS negative "small agranular lymphocytes" ARE natural killer cells. In: Journal of Immunology. 1991 ; Vol. 146, No. 11. pp. 4048-4052.
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