Cdc42 controls progenitor cell differentiation and β-catenin turnover in skin

Xunwei Wu, Fabio Quondamatteo, Tine Lefever, Aleksandra Czuchra, Hannelore Meyer, Anna Chrostek, Ralf Paus, Lutz Langbein, Cord Brakebusch

Research output: Contribution to journalArticle

127 Scopus citations

Abstract

Differentiation of skin stem cells into hair follicles (HFs) requires the inhibition of β-catenin degradation, which is controlled by a complex containing axin and the protein kinase GSK3β. Using conditional gene targeting in mice, we show now that the small GTPase Cdc42 is crucial for differentiation of skin progenitor cells into HF lineage and that it regulates the turnover of β-catenin. In the absence of Cdc42, degradation of β-catenin was increased corresponding to a decreased phosphorylation of GSK3β at Ser 9 and an increased phosphorylation of axin, which is known to be required for binding of β-catenin to the degradation machinery. Cdc42-mediated regulation of β-catenin turnover was completely dependent on PKCζ, which associated with Cdc42, Par6, and Par3. These data suggest that Cdc42 regulation of β-catenin turnover is important for terminal differentiation of HF progenitor cells in vivo.

Original languageEnglish (US)
Pages (from-to)571-585
Number of pages15
JournalGenes and Development
Volume20
Issue number5
DOIs
StatePublished - Mar 1 2006

Keywords

  • Keratinocytes
  • Rho GTPases
  • β-catenin

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Fingerprint Dive into the research topics of 'Cdc42 controls progenitor cell differentiation and β-catenin turnover in skin'. Together they form a unique fingerprint.

  • Cite this

    Wu, X., Quondamatteo, F., Lefever, T., Czuchra, A., Meyer, H., Chrostek, A., Paus, R., Langbein, L., & Brakebusch, C. (2006). Cdc42 controls progenitor cell differentiation and β-catenin turnover in skin. Genes and Development, 20(5), 571-585. https://doi.org/10.1101/gad.361406