CD8+ T-cell interleukin-7 receptor alpha expression as a potential indicator of disease status in HIV-infected children

Tanvi S. Sharma, Jane Hughes, Amarylis Murillo, Joanne Riley, Andrela Soares, Francesca Little, Charles D Mitchell, Willem A. Hanekom

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Initiation and modification of antiretroviral therapy in HIV-infected children depend on viral load and CD4+ T-cell count. However, these surrogates have limitations, and complementary immunological markers to assess therapeutic response are needed. Our aim was to evaluate CD8+ T-cell expression of CD127 as a marker of disease status in HIV-infected children, based on adult data suggesting its usefulness. We hypothesized that CD127 expression on CD8+ T-cells is lower in children with more advanced disease. Methods: In a cross-sectional evaluation, we used flow cytometry to measure CD127+ expression on CD8+ T-cells in whole blood from HIV-infected children with varying disease status. This was compared with expression of CD38 on this subset, currently used in clinical practice as a marker of disease status. Results: 51 HIV-infected children were enrolled. There was a strong positive correlation between CD127 expression on CD8+ T-cells and CD4+ T-cell count, and height and weight z-scores, and a strong negative correlation between CD127 expression and viral load. In contrast, we found no association between CD38 expression and these disease status markers. Conclusions: CD8+ T-cell CD127 expression is significantly higher in children with better HIV disease control, and may have a role as an immunologic indicator of disease status. Longitudinal studies are needed to determine the utility of this marker as a potential indicator of HIV disease progression.

Original languageEnglish
Article numbere3986
JournalPLoS One
Volume3
Issue number12
DOIs
StatePublished - Dec 19 2008

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Interleukin-7 Receptors
interleukin-7
T-cells
T-lymphocytes
HIV
T-Lymphocytes
receptors
CD4 Lymphocyte Count
viral load
Viral Load
Disease control
therapeutics
Flow cytometry
Immune System Diseases
longitudinal studies
disease course
Longitudinal Studies
flow cytometry
Disease Progression
Flow Cytometry

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Sharma, T. S., Hughes, J., Murillo, A., Riley, J., Soares, A., Little, F., ... Hanekom, W. A. (2008). CD8+ T-cell interleukin-7 receptor alpha expression as a potential indicator of disease status in HIV-infected children. PLoS One, 3(12), [e3986]. https://doi.org/10.1371/journal.pone.0003986

CD8+ T-cell interleukin-7 receptor alpha expression as a potential indicator of disease status in HIV-infected children. / Sharma, Tanvi S.; Hughes, Jane; Murillo, Amarylis; Riley, Joanne; Soares, Andrela; Little, Francesca; Mitchell, Charles D; Hanekom, Willem A.

In: PLoS One, Vol. 3, No. 12, e3986, 19.12.2008.

Research output: Contribution to journalArticle

Sharma, Tanvi S. ; Hughes, Jane ; Murillo, Amarylis ; Riley, Joanne ; Soares, Andrela ; Little, Francesca ; Mitchell, Charles D ; Hanekom, Willem A. / CD8+ T-cell interleukin-7 receptor alpha expression as a potential indicator of disease status in HIV-infected children. In: PLoS One. 2008 ; Vol. 3, No. 12.
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