CD40L-null NKT cells provide B cell help for specific antibody responses

Hemangi B. Shah, Sunil Joshi, Mark L. Lang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

CD1d-binding glycolipids exert potent adjuvant effects on T-dependent Ab responses. The mechanisms include cognate interaction between CD1d-expressing B cells and TCR-expressing Type I CD1d-restricted natural killer T cells (NKTs). However, the critical NKT-derived factors that stimulate B cells are poorly understood. We tested the hypothesis that CD1d-driven CD40L expression by NKT cells influences humoral immunity. Bone marrow chimeras with CD40L +/+ or CD40L -/- NKT cells were immunized with Ag plus CD1d ligand before measuring Ab responses. CD40L -/- NKT cells stimulated higher endpoint Ab titers than controls expressing CD40L. In contrast, immunization of CD40L -/- mice revealed that CD40L -/- NKT cells could not provide B cell help when Th cells lacked CD40L. We report that CD40L -/- NKT cells can provide help for Ab production and do so cooperatively with CD40L +/+ Th cells. We suggest that the manner in which NKT cells provide B cell help is distinct from that of Th cells.

Original languageEnglish (US)
Pages (from-to)9132-9136
Number of pages5
JournalVaccine
Volume29
Issue number49
DOIs
StatePublished - Nov 15 2011
Externally publishedYes

Fingerprint

Null Lymphocytes
CD40 Ligand
Natural Killer T-Cells
B-lymphocytes
Antibody Formation
B-Lymphocytes
antibodies
cells
chimerism
glycolipids
natural killer cells
Glycolipids
endpoints
humoral immunity
bone marrow
Humoral Immunity
adjuvants
immunization
T-lymphocytes
Immunization

Keywords

  • Antibody
  • B lymphocyte
  • CD40L
  • NKT cell

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine

Cite this

CD40L-null NKT cells provide B cell help for specific antibody responses. / Shah, Hemangi B.; Joshi, Sunil; Lang, Mark L.

In: Vaccine, Vol. 29, No. 49, 15.11.2011, p. 9132-9136.

Research output: Contribution to journalArticle

Shah, Hemangi B. ; Joshi, Sunil ; Lang, Mark L. / CD40L-null NKT cells provide B cell help for specific antibody responses. In: Vaccine. 2011 ; Vol. 29, No. 49. pp. 9132-9136.
@article{762e13b127e34f3ca30e0d77e55c695d,
title = "CD40L-null NKT cells provide B cell help for specific antibody responses",
abstract = "CD1d-binding glycolipids exert potent adjuvant effects on T-dependent Ab responses. The mechanisms include cognate interaction between CD1d-expressing B cells and TCR-expressing Type I CD1d-restricted natural killer T cells (NKTs). However, the critical NKT-derived factors that stimulate B cells are poorly understood. We tested the hypothesis that CD1d-driven CD40L expression by NKT cells influences humoral immunity. Bone marrow chimeras with CD40L +/+ or CD40L -/- NKT cells were immunized with Ag plus CD1d ligand before measuring Ab responses. CD40L -/- NKT cells stimulated higher endpoint Ab titers than controls expressing CD40L. In contrast, immunization of CD40L -/- mice revealed that CD40L -/- NKT cells could not provide B cell help when Th cells lacked CD40L. We report that CD40L -/- NKT cells can provide help for Ab production and do so cooperatively with CD40L +/+ Th cells. We suggest that the manner in which NKT cells provide B cell help is distinct from that of Th cells.",
keywords = "Antibody, B lymphocyte, CD40L, NKT cell",
author = "Shah, {Hemangi B.} and Sunil Joshi and Lang, {Mark L.}",
year = "2011",
month = "11",
day = "15",
doi = "10.1016/j.vaccine.2011.09.060",
language = "English (US)",
volume = "29",
pages = "9132--9136",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "49",

}

TY - JOUR

T1 - CD40L-null NKT cells provide B cell help for specific antibody responses

AU - Shah, Hemangi B.

AU - Joshi, Sunil

AU - Lang, Mark L.

PY - 2011/11/15

Y1 - 2011/11/15

N2 - CD1d-binding glycolipids exert potent adjuvant effects on T-dependent Ab responses. The mechanisms include cognate interaction between CD1d-expressing B cells and TCR-expressing Type I CD1d-restricted natural killer T cells (NKTs). However, the critical NKT-derived factors that stimulate B cells are poorly understood. We tested the hypothesis that CD1d-driven CD40L expression by NKT cells influences humoral immunity. Bone marrow chimeras with CD40L +/+ or CD40L -/- NKT cells were immunized with Ag plus CD1d ligand before measuring Ab responses. CD40L -/- NKT cells stimulated higher endpoint Ab titers than controls expressing CD40L. In contrast, immunization of CD40L -/- mice revealed that CD40L -/- NKT cells could not provide B cell help when Th cells lacked CD40L. We report that CD40L -/- NKT cells can provide help for Ab production and do so cooperatively with CD40L +/+ Th cells. We suggest that the manner in which NKT cells provide B cell help is distinct from that of Th cells.

AB - CD1d-binding glycolipids exert potent adjuvant effects on T-dependent Ab responses. The mechanisms include cognate interaction between CD1d-expressing B cells and TCR-expressing Type I CD1d-restricted natural killer T cells (NKTs). However, the critical NKT-derived factors that stimulate B cells are poorly understood. We tested the hypothesis that CD1d-driven CD40L expression by NKT cells influences humoral immunity. Bone marrow chimeras with CD40L +/+ or CD40L -/- NKT cells were immunized with Ag plus CD1d ligand before measuring Ab responses. CD40L -/- NKT cells stimulated higher endpoint Ab titers than controls expressing CD40L. In contrast, immunization of CD40L -/- mice revealed that CD40L -/- NKT cells could not provide B cell help when Th cells lacked CD40L. We report that CD40L -/- NKT cells can provide help for Ab production and do so cooperatively with CD40L +/+ Th cells. We suggest that the manner in which NKT cells provide B cell help is distinct from that of Th cells.

KW - Antibody

KW - B lymphocyte

KW - CD40L

KW - NKT cell

UR - http://www.scopus.com/inward/record.url?scp=82555187980&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=82555187980&partnerID=8YFLogxK

U2 - 10.1016/j.vaccine.2011.09.060

DO - 10.1016/j.vaccine.2011.09.060

M3 - Article

VL - 29

SP - 9132

EP - 9136

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 49

ER -