CD40L-null NKT cells provide B cell help for specific antibody responses

Hemangi B. Shah, Sunil K. Joshi, Mark L. Lang

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

CD1d-binding glycolipids exert potent adjuvant effects on T-dependent Ab responses. The mechanisms include cognate interaction between CD1d-expressing B cells and TCR-expressing Type I CD1d-restricted natural killer T cells (NKTs). However, the critical NKT-derived factors that stimulate B cells are poorly understood. We tested the hypothesis that CD1d-driven CD40L expression by NKT cells influences humoral immunity. Bone marrow chimeras with CD40L +/+ or CD40L -/- NKT cells were immunized with Ag plus CD1d ligand before measuring Ab responses. CD40L -/- NKT cells stimulated higher endpoint Ab titers than controls expressing CD40L. In contrast, immunization of CD40L -/- mice revealed that CD40L -/- NKT cells could not provide B cell help when Th cells lacked CD40L. We report that CD40L -/- NKT cells can provide help for Ab production and do so cooperatively with CD40L +/+ Th cells. We suggest that the manner in which NKT cells provide B cell help is distinct from that of Th cells.

Original languageEnglish (US)
Pages (from-to)9132-9136
Number of pages5
JournalVaccine
Volume29
Issue number49
DOIs
StatePublished - Nov 15 2011
Externally publishedYes

Keywords

  • Antibody
  • B lymphocyte
  • CD40L
  • NKT cell

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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