CD40L expressed from the canarypox vector, ALVAC, can boost immunogenicity of HIV-1 canarypox vaccine in mice and enhance the in vitro expansion of viral specific CD8+ T cell memory responses from HIV-1-infected and HIV-1-uninfected individuals

Jun Liu, Qigui Yu, Geoffrey Stone, Feng Yun Yue, Nicholas Ngai, R. Brad Jones, Richard S. Kornbluth, Mario A. Ostrowski

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Human immunodeficiency virus type 1 (HIV-1) canarypox vaccines are safe but poorly immunogenic. CD40 ligand (CD40L), a member of the tumor necrosis factor superfamily (TNFSF), is a pivotal costimulatory molecule for immune responses. To explore whether CD40L can be used as an adjuvant for HIV-1 canarypox vaccine, we constructed recombinant canarypox viruses expressing CD40L. Co-immunization of mice with CD40L expressing canarypox and the canarypox vaccine expressing HIV-1 proteins, vCP1452, augmented HIV-1 specific cytotoxic T lymphocyte (CTL) responses in terms of frequency, polyfunctionality and interleukin (IL)-7 receptor α chain (IL-7Rα, CD127) expression. In addition, CD40L expressed from canarypox virus could significantly augment CD4+ T cell responses against HIV-1 in mice. CD40L expressed from canarypox virus matured human monocyte-derived dendritic cells (MDDCs) in a tumor necrosis factor-α (TNF-α) independent manner, which underwent less apoptosis, and could expand ex vivo Epstein-Barr virus (EBV)-specific CTL responses from healthy human individuals and ex vivo HIV-1-specific CTL responses from HIV-1-infected individuals in the presence or absence of CD4+ T cells. Taken together, our results suggest that CD40L incorporation into poxvirus vectors could be used as a strategy to enhance their immunogenicity.

Original languageEnglish
Pages (from-to)4062-4072
Number of pages11
JournalVaccine
Volume26
Issue number32
DOIs
StatePublished - Jul 29 2008
Externally publishedYes

Fingerprint

CD40 Ligand
Human immunodeficiency virus 1
HIV-1
Vaccines
T-lymphocytes
Canarypox virus
immune response
vaccines
T-Lymphocytes
mice
cytotoxic T-lymphocytes
Cytotoxic T-Lymphocytes
tumor necrosis factors
Tumor Necrosis Factor-alpha
Interleukin-7 Receptors
Poxviridae
interleukin-7
Human Immunodeficiency Virus Proteins
Human herpesvirus 4
Synthetic Vaccines

Keywords

  • AIDS
  • Canarypox vector
  • Cytotoxic T cell

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

CD40L expressed from the canarypox vector, ALVAC, can boost immunogenicity of HIV-1 canarypox vaccine in mice and enhance the in vitro expansion of viral specific CD8+ T cell memory responses from HIV-1-infected and HIV-1-uninfected individuals. / Liu, Jun; Yu, Qigui; Stone, Geoffrey; Yue, Feng Yun; Ngai, Nicholas; Jones, R. Brad; Kornbluth, Richard S.; Ostrowski, Mario A.

In: Vaccine, Vol. 26, No. 32, 29.07.2008, p. 4062-4072.

Research output: Contribution to journalArticle

Liu, Jun ; Yu, Qigui ; Stone, Geoffrey ; Yue, Feng Yun ; Ngai, Nicholas ; Jones, R. Brad ; Kornbluth, Richard S. ; Ostrowski, Mario A. / CD40L expressed from the canarypox vector, ALVAC, can boost immunogenicity of HIV-1 canarypox vaccine in mice and enhance the in vitro expansion of viral specific CD8+ T cell memory responses from HIV-1-infected and HIV-1-uninfected individuals. In: Vaccine. 2008 ; Vol. 26, No. 32. pp. 4062-4072.
@article{85d006c6bf184337ad12ec0f21c18873,
title = "CD40L expressed from the canarypox vector, ALVAC, can boost immunogenicity of HIV-1 canarypox vaccine in mice and enhance the in vitro expansion of viral specific CD8+ T cell memory responses from HIV-1-infected and HIV-1-uninfected individuals",
abstract = "Human immunodeficiency virus type 1 (HIV-1) canarypox vaccines are safe but poorly immunogenic. CD40 ligand (CD40L), a member of the tumor necrosis factor superfamily (TNFSF), is a pivotal costimulatory molecule for immune responses. To explore whether CD40L can be used as an adjuvant for HIV-1 canarypox vaccine, we constructed recombinant canarypox viruses expressing CD40L. Co-immunization of mice with CD40L expressing canarypox and the canarypox vaccine expressing HIV-1 proteins, vCP1452, augmented HIV-1 specific cytotoxic T lymphocyte (CTL) responses in terms of frequency, polyfunctionality and interleukin (IL)-7 receptor α chain (IL-7Rα, CD127) expression. In addition, CD40L expressed from canarypox virus could significantly augment CD4+ T cell responses against HIV-1 in mice. CD40L expressed from canarypox virus matured human monocyte-derived dendritic cells (MDDCs) in a tumor necrosis factor-α (TNF-α) independent manner, which underwent less apoptosis, and could expand ex vivo Epstein-Barr virus (EBV)-specific CTL responses from healthy human individuals and ex vivo HIV-1-specific CTL responses from HIV-1-infected individuals in the presence or absence of CD4+ T cells. Taken together, our results suggest that CD40L incorporation into poxvirus vectors could be used as a strategy to enhance their immunogenicity.",
keywords = "AIDS, Canarypox vector, Cytotoxic T cell",
author = "Jun Liu and Qigui Yu and Geoffrey Stone and Yue, {Feng Yun} and Nicholas Ngai and Jones, {R. Brad} and Kornbluth, {Richard S.} and Ostrowski, {Mario A.}",
year = "2008",
month = "7",
day = "29",
doi = "10.1016/j.vaccine.2008.05.018",
language = "English",
volume = "26",
pages = "4062--4072",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "32",

}

TY - JOUR

T1 - CD40L expressed from the canarypox vector, ALVAC, can boost immunogenicity of HIV-1 canarypox vaccine in mice and enhance the in vitro expansion of viral specific CD8+ T cell memory responses from HIV-1-infected and HIV-1-uninfected individuals

AU - Liu, Jun

AU - Yu, Qigui

AU - Stone, Geoffrey

AU - Yue, Feng Yun

AU - Ngai, Nicholas

AU - Jones, R. Brad

AU - Kornbluth, Richard S.

AU - Ostrowski, Mario A.

PY - 2008/7/29

Y1 - 2008/7/29

N2 - Human immunodeficiency virus type 1 (HIV-1) canarypox vaccines are safe but poorly immunogenic. CD40 ligand (CD40L), a member of the tumor necrosis factor superfamily (TNFSF), is a pivotal costimulatory molecule for immune responses. To explore whether CD40L can be used as an adjuvant for HIV-1 canarypox vaccine, we constructed recombinant canarypox viruses expressing CD40L. Co-immunization of mice with CD40L expressing canarypox and the canarypox vaccine expressing HIV-1 proteins, vCP1452, augmented HIV-1 specific cytotoxic T lymphocyte (CTL) responses in terms of frequency, polyfunctionality and interleukin (IL)-7 receptor α chain (IL-7Rα, CD127) expression. In addition, CD40L expressed from canarypox virus could significantly augment CD4+ T cell responses against HIV-1 in mice. CD40L expressed from canarypox virus matured human monocyte-derived dendritic cells (MDDCs) in a tumor necrosis factor-α (TNF-α) independent manner, which underwent less apoptosis, and could expand ex vivo Epstein-Barr virus (EBV)-specific CTL responses from healthy human individuals and ex vivo HIV-1-specific CTL responses from HIV-1-infected individuals in the presence or absence of CD4+ T cells. Taken together, our results suggest that CD40L incorporation into poxvirus vectors could be used as a strategy to enhance their immunogenicity.

AB - Human immunodeficiency virus type 1 (HIV-1) canarypox vaccines are safe but poorly immunogenic. CD40 ligand (CD40L), a member of the tumor necrosis factor superfamily (TNFSF), is a pivotal costimulatory molecule for immune responses. To explore whether CD40L can be used as an adjuvant for HIV-1 canarypox vaccine, we constructed recombinant canarypox viruses expressing CD40L. Co-immunization of mice with CD40L expressing canarypox and the canarypox vaccine expressing HIV-1 proteins, vCP1452, augmented HIV-1 specific cytotoxic T lymphocyte (CTL) responses in terms of frequency, polyfunctionality and interleukin (IL)-7 receptor α chain (IL-7Rα, CD127) expression. In addition, CD40L expressed from canarypox virus could significantly augment CD4+ T cell responses against HIV-1 in mice. CD40L expressed from canarypox virus matured human monocyte-derived dendritic cells (MDDCs) in a tumor necrosis factor-α (TNF-α) independent manner, which underwent less apoptosis, and could expand ex vivo Epstein-Barr virus (EBV)-specific CTL responses from healthy human individuals and ex vivo HIV-1-specific CTL responses from HIV-1-infected individuals in the presence or absence of CD4+ T cells. Taken together, our results suggest that CD40L incorporation into poxvirus vectors could be used as a strategy to enhance their immunogenicity.

KW - AIDS

KW - Canarypox vector

KW - Cytotoxic T cell

UR - http://www.scopus.com/inward/record.url?scp=47149112188&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=47149112188&partnerID=8YFLogxK

U2 - 10.1016/j.vaccine.2008.05.018

DO - 10.1016/j.vaccine.2008.05.018

M3 - Article

C2 - 18562053

AN - SCOPUS:47149112188

VL - 26

SP - 4062

EP - 4072

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 32

ER -