CD32B is highly expressed on clonal plasma cells from patients with systemic light-chain amyloidosis and provides a target for monoclonal antibody-based therapy

Ping Zhou, Raymond L. Comenzo, Adam B. Olshen, Ezio Bonvini, Scott Koenig, Peter G. Maslak, Martin Fleisher, James Hoffman, Suresh Jhanwar, James W. Young, Stephen D. Nimer, Adam M. Boruchov

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Despite advances in therapy, many patients with systemic light-chain amyloidosis (AL) die within 3 years from diagnosis. The humanized 2B6 monoclonal antibody (MoAb) is specific for the low-affinity IgG Fc receptor CD32B and effective in a human CD32B + B-cell lymphoma murine xenograft model. Because MoAb therapy could improve outcomes in AL, we studied CD32B expression by clonal plasma cells obtained from 48 patients with AL. Transcript profiling showed that expression of CD32B was significantly higher than expression of all other Fc receptor family members. Reverse-transcriptase polymerase chain reaction (RT-PCR) using double-enriched CD138 + plasma cells showed uniform expression of the stable cell surface CD32B1 isoform at diagnosis and relapse, and flow cytometry showed intense CD32B cell surface staining on 99% of CD138 + plasma cells at diagnosis and relapse. These data provide a rationale for the novel therapeutic targeting of CD32B using the humanized 2B6 MoAb in patients with systemic AL-amyloidosis.

Original languageEnglish (US)
Pages (from-to)3403-3406
Number of pages4
JournalBlood
Volume111
Issue number7
DOIs
StatePublished - Apr 1 2008

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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    Zhou, P., Comenzo, R. L., Olshen, A. B., Bonvini, E., Koenig, S., Maslak, P. G., Fleisher, M., Hoffman, J., Jhanwar, S., Young, J. W., Nimer, S. D., & Boruchov, A. M. (2008). CD32B is highly expressed on clonal plasma cells from patients with systemic light-chain amyloidosis and provides a target for monoclonal antibody-based therapy. Blood, 111(7), 3403-3406. https://doi.org/10.1182/blood-2007-11-125526