The elimination of self-reactive T-cells in the thymus requires signals from the T-cell receptor together with costimulatory signals that have not yet been identified. We investigated the potential role of CD30 as costimulator for negative selection. Anti-CD3 mAb injection was used to simulate a model for negative selection in mice. In vivo administration of anti-CD3 selectively depletes CD4+ CD8+ thymocytes while having minimal effects on the mature single positive thymocytes. CD3-marker analysis by FACS reveals that the a.nti-CI>3 sensitive population expresses low and intermediate levels of CDS and the resistant populations to be high CDS expressors. Injection of antiCD30 or CD30-Ig prior to anti-CD3 administration results in the survival of a group of CD4, CDS double positive thymocytes that exhibit intermediate CD3 expression. In addition, CD30-marker analysis reveals the presence of CD30 in immunohistochemistry of adult thymic sections and on thymocytes expressing CDS at intermediate levels. Our results are consistent with an essential function of CD30 as costimulator during negative selection of thymocytes.
|Original language||English (US)|
|State||Published - Dec 1 1996|
ASJC Scopus subject areas
- Molecular Biology