CD30 costimulation is required for negative selection in the thymus

T. Nguyen, Rebecca D Adkins, E. R. Podack

Research output: Contribution to journalArticle

Abstract

The elimination of self-reactive T-cells in the thymus requires signals from the T-cell receptor together with costimulatory signals that have not yet been identified. We investigated the potential role of CD30 as costimulator for negative selection. Anti-CD3 mAb injection was used to simulate a model for negative selection in mice. In vivo administration of anti-CD3 selectively depletes CD4+ CD8+ thymocytes while having minimal effects on the mature single positive thymocytes. CD3-marker analysis by FACS reveals that the a.nti-CI>3 sensitive population expresses low and intermediate levels of CDS and the resistant populations to be high CDS expressors. Injection of antiCD30 or CD30-Ig prior to anti-CD3 administration results in the survival of a group of CD4, CDS double positive thymocytes that exhibit intermediate CD3 expression. In addition, CD30-marker analysis reveals the presence of CD30 in immunohistochemistry of adult thymic sections and on thymocytes expressing CDS at intermediate levels. Our results are consistent with an essential function of CD30 as costimulator during negative selection of thymocytes.

Original languageEnglish
JournalFASEB Journal
Volume10
Issue number6
StatePublished - Dec 1 1996

Fingerprint

Thymus
thymocytes
Thymocytes
Thymus Gland
T-cells
T-Cell Antigen Receptor
T-lymphocytes
injection
Injections
Vulnerable Populations
immunohistochemistry
Immunohistochemistry
T-Lymphocytes
receptors
Survival
mice
Population

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Nguyen, T., Adkins, R. D., & Podack, E. R. (1996). CD30 costimulation is required for negative selection in the thymus. FASEB Journal, 10(6).

CD30 costimulation is required for negative selection in the thymus. / Nguyen, T.; Adkins, Rebecca D; Podack, E. R.

In: FASEB Journal, Vol. 10, No. 6, 01.12.1996.

Research output: Contribution to journalArticle

Nguyen, T, Adkins, RD & Podack, ER 1996, 'CD30 costimulation is required for negative selection in the thymus', FASEB Journal, vol. 10, no. 6.
Nguyen, T. ; Adkins, Rebecca D ; Podack, E. R. / CD30 costimulation is required for negative selection in the thymus. In: FASEB Journal. 1996 ; Vol. 10, No. 6.
@article{515ef753b4d04b3083406b8a3e413af0,
title = "CD30 costimulation is required for negative selection in the thymus",
abstract = "The elimination of self-reactive T-cells in the thymus requires signals from the T-cell receptor together with costimulatory signals that have not yet been identified. We investigated the potential role of CD30 as costimulator for negative selection. Anti-CD3 mAb injection was used to simulate a model for negative selection in mice. In vivo administration of anti-CD3 selectively depletes CD4+ CD8+ thymocytes while having minimal effects on the mature single positive thymocytes. CD3-marker analysis by FACS reveals that the a.nti-CI>3 sensitive population expresses low and intermediate levels of CDS and the resistant populations to be high CDS expressors. Injection of antiCD30 or CD30-Ig prior to anti-CD3 administration results in the survival of a group of CD4, CDS double positive thymocytes that exhibit intermediate CD3 expression. In addition, CD30-marker analysis reveals the presence of CD30 in immunohistochemistry of adult thymic sections and on thymocytes expressing CDS at intermediate levels. Our results are consistent with an essential function of CD30 as costimulator during negative selection of thymocytes.",
author = "T. Nguyen and Adkins, {Rebecca D} and Podack, {E. R.}",
year = "1996",
month = "12",
day = "1",
language = "English",
volume = "10",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "6",

}

TY - JOUR

T1 - CD30 costimulation is required for negative selection in the thymus

AU - Nguyen, T.

AU - Adkins, Rebecca D

AU - Podack, E. R.

PY - 1996/12/1

Y1 - 1996/12/1

N2 - The elimination of self-reactive T-cells in the thymus requires signals from the T-cell receptor together with costimulatory signals that have not yet been identified. We investigated the potential role of CD30 as costimulator for negative selection. Anti-CD3 mAb injection was used to simulate a model for negative selection in mice. In vivo administration of anti-CD3 selectively depletes CD4+ CD8+ thymocytes while having minimal effects on the mature single positive thymocytes. CD3-marker analysis by FACS reveals that the a.nti-CI>3 sensitive population expresses low and intermediate levels of CDS and the resistant populations to be high CDS expressors. Injection of antiCD30 or CD30-Ig prior to anti-CD3 administration results in the survival of a group of CD4, CDS double positive thymocytes that exhibit intermediate CD3 expression. In addition, CD30-marker analysis reveals the presence of CD30 in immunohistochemistry of adult thymic sections and on thymocytes expressing CDS at intermediate levels. Our results are consistent with an essential function of CD30 as costimulator during negative selection of thymocytes.

AB - The elimination of self-reactive T-cells in the thymus requires signals from the T-cell receptor together with costimulatory signals that have not yet been identified. We investigated the potential role of CD30 as costimulator for negative selection. Anti-CD3 mAb injection was used to simulate a model for negative selection in mice. In vivo administration of anti-CD3 selectively depletes CD4+ CD8+ thymocytes while having minimal effects on the mature single positive thymocytes. CD3-marker analysis by FACS reveals that the a.nti-CI>3 sensitive population expresses low and intermediate levels of CDS and the resistant populations to be high CDS expressors. Injection of antiCD30 or CD30-Ig prior to anti-CD3 administration results in the survival of a group of CD4, CDS double positive thymocytes that exhibit intermediate CD3 expression. In addition, CD30-marker analysis reveals the presence of CD30 in immunohistochemistry of adult thymic sections and on thymocytes expressing CDS at intermediate levels. Our results are consistent with an essential function of CD30 as costimulator during negative selection of thymocytes.

UR - http://www.scopus.com/inward/record.url?scp=33748893429&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748893429&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33748893429

VL - 10

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 6

ER -