CD2AP in mouse and human podocytes controls a proteolytic program that regulates cytoskeletal structure and cellular survival

Suma Yaddanapudi, Mehmet M. Altintas, Andreas D. Kistler, Isabel Fernandez, Clemens C. Möller, Changli Wei, Vasil Peev, Jan B. Flesche, Anna Lena Forst, Jing Li, Jaakko Patrakka, Zhijie Xiao, Florian Grahammer, Mario Schiffer, Tobias Lohmüller, Thomas Reinheckel, Changkyu Gu, Tobias B. Huber, Wenjun Ju, Markus BitzerMaria P. Rastaldi, Phillip Ruiz, Karl Tryggvason, Andrey S. Shaw, Christian H Faul, Sanja Sever, Jochen Reiser

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Abstract

Kidney podocytes are highly differentiated epithelial cells that form interdigitating foot processes with bridging slit diaphragms (SDs) that regulate renal ultrafiltration. Podocyte injury results in proteinuric kidney disease, and genetic deletion of SD-associated CD2-associated protein (CD2AP) leads to progressive renal failure in mice and humans. Here, we have shown that CD2AP regulates the TGF-β1-dependent translocation of dendrin from the SD to the nucleus. Nuclear dendrin acted as a transcription factor to promote expression of cytosolic cathepsin L (CatL). CatL proteolyzed the regulatory GTPase dynamin and the actin-associated adapter synaptopodin, leading to a reorganization of the podocyte microfilament system and consequent proteinuria. CD2AP itself was proteolyzed by CatL, promoting sustained expression of the protease during podocyte injury, and in turn increasing the apoptotic susceptibility of podocytes to TGF-β1. Our study identifies CD2AP as the gatekeeper of the podocyte TGF-β response through its regulation of CatL expression and defines a molecular mechanism underlying proteinuric kidney disease.

Original languageEnglish
Pages (from-to)3965-3980
Number of pages16
JournalJournal of Clinical Investigation
Volume121
Issue number10
DOIs
StatePublished - Oct 3 2011

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Podocytes
Cellular Structures
Cathepsin L
Diaphragm
Kidney Diseases
Dynamins
Kidney
GTP Phosphohydrolases
Wounds and Injuries
Ultrafiltration
Actin Cytoskeleton
Proteinuria
Renal Insufficiency
CD2-associated protein
Actins
Foot
Peptide Hydrolases
Transcription Factors
Epithelial Cells

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Yaddanapudi, S., Altintas, M. M., Kistler, A. D., Fernandez, I., Möller, C. C., Wei, C., ... Reiser, J. (2011). CD2AP in mouse and human podocytes controls a proteolytic program that regulates cytoskeletal structure and cellular survival. Journal of Clinical Investigation, 121(10), 3965-3980. https://doi.org/10.1172/JCI58552

CD2AP in mouse and human podocytes controls a proteolytic program that regulates cytoskeletal structure and cellular survival. / Yaddanapudi, Suma; Altintas, Mehmet M.; Kistler, Andreas D.; Fernandez, Isabel; Möller, Clemens C.; Wei, Changli; Peev, Vasil; Flesche, Jan B.; Forst, Anna Lena; Li, Jing; Patrakka, Jaakko; Xiao, Zhijie; Grahammer, Florian; Schiffer, Mario; Lohmüller, Tobias; Reinheckel, Thomas; Gu, Changkyu; Huber, Tobias B.; Ju, Wenjun; Bitzer, Markus; Rastaldi, Maria P.; Ruiz, Phillip; Tryggvason, Karl; Shaw, Andrey S.; Faul, Christian H; Sever, Sanja; Reiser, Jochen.

In: Journal of Clinical Investigation, Vol. 121, No. 10, 03.10.2011, p. 3965-3980.

Research output: Contribution to journalArticle

Yaddanapudi, S, Altintas, MM, Kistler, AD, Fernandez, I, Möller, CC, Wei, C, Peev, V, Flesche, JB, Forst, AL, Li, J, Patrakka, J, Xiao, Z, Grahammer, F, Schiffer, M, Lohmüller, T, Reinheckel, T, Gu, C, Huber, TB, Ju, W, Bitzer, M, Rastaldi, MP, Ruiz, P, Tryggvason, K, Shaw, AS, Faul, CH, Sever, S & Reiser, J 2011, 'CD2AP in mouse and human podocytes controls a proteolytic program that regulates cytoskeletal structure and cellular survival', Journal of Clinical Investigation, vol. 121, no. 10, pp. 3965-3980. https://doi.org/10.1172/JCI58552
Yaddanapudi, Suma ; Altintas, Mehmet M. ; Kistler, Andreas D. ; Fernandez, Isabel ; Möller, Clemens C. ; Wei, Changli ; Peev, Vasil ; Flesche, Jan B. ; Forst, Anna Lena ; Li, Jing ; Patrakka, Jaakko ; Xiao, Zhijie ; Grahammer, Florian ; Schiffer, Mario ; Lohmüller, Tobias ; Reinheckel, Thomas ; Gu, Changkyu ; Huber, Tobias B. ; Ju, Wenjun ; Bitzer, Markus ; Rastaldi, Maria P. ; Ruiz, Phillip ; Tryggvason, Karl ; Shaw, Andrey S. ; Faul, Christian H ; Sever, Sanja ; Reiser, Jochen. / CD2AP in mouse and human podocytes controls a proteolytic program that regulates cytoskeletal structure and cellular survival. In: Journal of Clinical Investigation. 2011 ; Vol. 121, No. 10. pp. 3965-3980.
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