CD27/CD70 interaction directly drives B cell IgG and IgM synthesis

Kazunaga Agematsu, Tetsuji Kobata, Feng-Chun Yang, Takayuki Nakazawa, Keitaro Fukushima, Masashi Kitahara, Tetsuo Mori, Kanji Sugita, Chikao Morimoto, Atsushi Komiyama

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

CD27 is a T cell activation antigen expressed on a majority of peripheral blood T cells. CD27 is also expressed on a subpopulation of human B cells, and it is reported that CD27+ B cells secrete both IgG and IgM. CD70, a ligand for CD27, is expressed on activated T and B cells, suggesting an interaction between T and B cells via CD27/CD70 ligation. Here, we analyze B cell immunoglobulin synthesis using a CD70 transfectant and present functional data showing that B cells secrete large amounts of IgG and IgM as a result of the CD27/CD70 interaction. A flow cytometric analysis showed that CD27 expression was increased and CD70 was expressed on tonsillar and peripheral blood B cells after activation with Staphylococcus aureus Cowan strain (SAC) plus interleukin (IL-2). In addition, the proliferation of B cells was enhanced mildly by the addition of CD70 transfectant, and its proliferation was blocked by anti-CD70 mAb. More importantly, the CD70 transfectant enhanced IgG and IgM production by purified B cells greatly in the presence of SAC plus IL-2. The enhancement was completely blocked by the addition of either anti-CD70 mAb or anti-CD27 mAb. Strongly suggesting that the interaction of CD27 with its ligand, CD70, on B cells plays an important role in B cell growth and differentiation to produce IgG and IgM.

Original languageEnglish (US)
Pages (from-to)2825-2829
Number of pages5
JournalEuropean Journal of Immunology
Volume25
Issue number10
DOIs
StatePublished - Oct 1995
Externally publishedYes

Fingerprint

Immunoglobulin M
B-Lymphocytes
Immunoglobulin G
Interleukin-2
T-Lymphocytes
CD70 Antigens
Staphylococcus aureus
Blood Cells
CD27 Antigens
Cell Communication
Ligation
Immunoglobulins
Cell Differentiation
Ligands
Growth

Keywords

  • CD27
  • CD70
  • Immunoglobulin

ASJC Scopus subject areas

  • Immunology

Cite this

Agematsu, K., Kobata, T., Yang, F-C., Nakazawa, T., Fukushima, K., Kitahara, M., ... Komiyama, A. (1995). CD27/CD70 interaction directly drives B cell IgG and IgM synthesis. European Journal of Immunology, 25(10), 2825-2829. https://doi.org/10.1002/eji.1830251017

CD27/CD70 interaction directly drives B cell IgG and IgM synthesis. / Agematsu, Kazunaga; Kobata, Tetsuji; Yang, Feng-Chun; Nakazawa, Takayuki; Fukushima, Keitaro; Kitahara, Masashi; Mori, Tetsuo; Sugita, Kanji; Morimoto, Chikao; Komiyama, Atsushi.

In: European Journal of Immunology, Vol. 25, No. 10, 10.1995, p. 2825-2829.

Research output: Contribution to journalArticle

Agematsu, K, Kobata, T, Yang, F-C, Nakazawa, T, Fukushima, K, Kitahara, M, Mori, T, Sugita, K, Morimoto, C & Komiyama, A 1995, 'CD27/CD70 interaction directly drives B cell IgG and IgM synthesis', European Journal of Immunology, vol. 25, no. 10, pp. 2825-2829. https://doi.org/10.1002/eji.1830251017
Agematsu K, Kobata T, Yang F-C, Nakazawa T, Fukushima K, Kitahara M et al. CD27/CD70 interaction directly drives B cell IgG and IgM synthesis. European Journal of Immunology. 1995 Oct;25(10):2825-2829. https://doi.org/10.1002/eji.1830251017
Agematsu, Kazunaga ; Kobata, Tetsuji ; Yang, Feng-Chun ; Nakazawa, Takayuki ; Fukushima, Keitaro ; Kitahara, Masashi ; Mori, Tetsuo ; Sugita, Kanji ; Morimoto, Chikao ; Komiyama, Atsushi. / CD27/CD70 interaction directly drives B cell IgG and IgM synthesis. In: European Journal of Immunology. 1995 ; Vol. 25, No. 10. pp. 2825-2829.
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