CD27/CD70 interaction directly drives B cell IgG and IgM synthesis

Kazunaga Agematsu, Tetsuji Kobata, Feng-Chun Yang, Takayuki Nakazawa, Keitaro Fukushima, Masashi Kitahara, Tetsuo Mori, Kanji Sugita, Chikao Morimoto, Atsushi Komiyama

Research output: Contribution to journalArticle

75 Scopus citations


CD27 is a T cell activation antigen expressed on a majority of peripheral blood T cells. CD27 is also expressed on a subpopulation of human B cells, and it is reported that CD27+ B cells secrete both IgG and IgM. CD70, a ligand for CD27, is expressed on activated T and B cells, suggesting an interaction between T and B cells via CD27/CD70 ligation. Here, we analyze B cell immunoglobulin synthesis using a CD70 transfectant and present functional data showing that B cells secrete large amounts of IgG and IgM as a result of the CD27/CD70 interaction. A flow cytometric analysis showed that CD27 expression was increased and CD70 was expressed on tonsillar and peripheral blood B cells after activation with Staphylococcus aureus Cowan strain (SAC) plus interleukin (IL-2). In addition, the proliferation of B cells was enhanced mildly by the addition of CD70 transfectant, and its proliferation was blocked by anti-CD70 mAb. More importantly, the CD70 transfectant enhanced IgG and IgM production by purified B cells greatly in the presence of SAC plus IL-2. The enhancement was completely blocked by the addition of either anti-CD70 mAb or anti-CD27 mAb. Strongly suggesting that the interaction of CD27 with its ligand, CD70, on B cells plays an important role in B cell growth and differentiation to produce IgG and IgM.

Original languageEnglish (US)
Pages (from-to)2825-2829
Number of pages5
JournalEuropean Journal of Immunology
Issue number10
StatePublished - Oct 1995
Externally publishedYes



  • CD27
  • CD70
  • Immunoglobulin

ASJC Scopus subject areas

  • Immunology

Cite this

Agematsu, K., Kobata, T., Yang, F-C., Nakazawa, T., Fukushima, K., Kitahara, M., Mori, T., Sugita, K., Morimoto, C., & Komiyama, A. (1995). CD27/CD70 interaction directly drives B cell IgG and IgM synthesis. European Journal of Immunology, 25(10), 2825-2829.