CD11b+ neutrophils predominate over RAM11+ macrophages in stretch-injured muscle

Barbara St Pierre Schneider, Stacey Brickson, David T. Corr, Thomas Best

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The purpose of this study was to determine whether both neutrophils and macrophages infiltrate the hematoma site of stretch-injured rabbit tibialis anterior muscle. The Mab.198 antibody was used to detect CD11b+ neutrophils or macrophages. Neutrophils were identified specifically by using the RPN3/57 antibody. The RAM11 antibody was used to detect macrophages. The histological characteristics of the hematoma site, torn fibers or inflammatory cells, were present primarily at 4 and 24 h, but not at 48 and 72 h after injury. A difference in the Mab.198+ cellular concentration was detected over time between uninjured and injured muscles (P = 0.03). The injured-uninjured difference in the RPN3/57+ or RAM11+ cellular concentrations approached significance (P = 0.07) or else was deemed insignificant (P = 0.13), respectively. Therefore, neutrophils may predominate over RAM11+ macrophages in stretch-injured muscle. These findings may influence the antiinflammatory strategies used to treat stretch injuries.

Original languageEnglish (US)
Pages (from-to)837-844
Number of pages8
JournalMuscle and Nerve
Volume25
Issue number6
DOIs
StatePublished - Jun 11 2002
Externally publishedYes

Keywords

  • Inflammation
  • Leukocytes
  • Muscle healing
  • RPN3/57
  • Stretch injury

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)

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