CD11b+ neutrophils predominate over RAM11+ macrophages in stretch-injured muscle

Barbara St Pierre Schneider, Stacey Brickson, David T. Corr, Thomas Best

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The purpose of this study was to determine whether both neutrophils and macrophages infiltrate the hematoma site of stretch-injured rabbit tibialis anterior muscle. The Mab.198 antibody was used to detect CD11b+ neutrophils or macrophages. Neutrophils were identified specifically by using the RPN3/57 antibody. The RAM11 antibody was used to detect macrophages. The histological characteristics of the hematoma site, torn fibers or inflammatory cells, were present primarily at 4 and 24 h, but not at 48 and 72 h after injury. A difference in the Mab.198+ cellular concentration was detected over time between uninjured and injured muscles (P = 0.03). The injured-uninjured difference in the RPN3/57+ or RAM11+ cellular concentrations approached significance (P = 0.07) or else was deemed insignificant (P = 0.13), respectively. Therefore, neutrophils may predominate over RAM11+ macrophages in stretch-injured muscle. These findings may influence the antiinflammatory strategies used to treat stretch injuries.

Original languageEnglish (US)
Pages (from-to)837-844
Number of pages8
JournalMuscle and Nerve
Volume25
Issue number6
DOIs
StatePublished - Jun 11 2002
Externally publishedYes

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Neutrophils
Macrophages
Muscles
Hematoma
Antibodies
Wounds and Injuries
Anti-Inflammatory Agents
Rabbits

Keywords

  • Inflammation
  • Leukocytes
  • Muscle healing
  • RPN3/57
  • Stretch injury

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)

Cite this

CD11b+ neutrophils predominate over RAM11+ macrophages in stretch-injured muscle. / Schneider, Barbara St Pierre; Brickson, Stacey; Corr, David T.; Best, Thomas.

In: Muscle and Nerve, Vol. 25, No. 6, 11.06.2002, p. 837-844.

Research output: Contribution to journalArticle

Schneider, Barbara St Pierre ; Brickson, Stacey ; Corr, David T. ; Best, Thomas. / CD11b+ neutrophils predominate over RAM11+ macrophages in stretch-injured muscle. In: Muscle and Nerve. 2002 ; Vol. 25, No. 6. pp. 837-844.
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