CCR5- and CXCR4-utilizing strains of human immunodeficiency virus type 1 exhibit differential tropism and pathogenesis in vivo

Robert D. Berkowitz, Sabina Alexander, Cris Bare, Valerie Linquist-Stepps, Mark Bogan, Mary E. Moreno, Lisa Gibson, Eric D. Wieder, Jon Kosek, Cheryl A. Stoddart, Joseph M. Mccune

Research output: Contribution to journalArticle

86 Scopus citations

Abstract

CCR5-utilizing (RS) and CXCR4-utilizing (X4) strains of human immunodeficiency virus type 1 (HIV-1) have been studied intensively in vitro, but the pathologic correlates of such differential tropism in vivo remain incompletely defined. In this study, X4 and R5 strains of HIV-1 were compared for tropism and pathogenesis in SCID-hu Thy/Liv mice, an in vivo model of human thymopoiesis. The X4 strain NL4-3 replicates quickly and extensively in thymocytes in the cortex and medulla, causing significant depletion. In contrast, the R5 strain Ba-L initially infects stromal cells including macrophages in the thymic medulla, without any obvious pathologic consequence. After a period of 3 to 4 weeks, Ba-L infection slowly spreads through the thymocyte populations, occasionally culminating in thymocyte depletion after week 6 of infection. During the entire time of infection, Ba- L did not mutate into variants capable of utilizing CXCR4. Therefore, X4 strains are highly cytopathic after infection of the human thymus. In contrast, infection with R5 strains of HIV-1 can result in a two-phase process in vivo, involving apparently nonpathogenic replication in medullary stromal cells followed by cytopathic replication in thymocytes.

Original languageEnglish (US)
Pages (from-to)10108-10117
Number of pages10
JournalJournal of virology
Volume72
Issue number12
StatePublished - Dec 1 1998
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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    Berkowitz, R. D., Alexander, S., Bare, C., Linquist-Stepps, V., Bogan, M., Moreno, M. E., Gibson, L., Wieder, E. D., Kosek, J., Stoddart, C. A., & Mccune, J. M. (1998). CCR5- and CXCR4-utilizing strains of human immunodeficiency virus type 1 exhibit differential tropism and pathogenesis in vivo. Journal of virology, 72(12), 10108-10117.