CCN3 controls 3D spatial localization of melanocytes in the human skin through DDR1

Mizuho Fukunaga-Kalabis, Gabriela Martinez, Zhao Jun Liu, Jiri Kalabis, Paul Mrass, Wolfgang Weninger, Sue M. Firth, Nathalie Planque, Bernard Perbal, Meenhard Herlyn

Research output: Contribution to journalArticle

72 Scopus citations

Abstract

Melanocytes reside within the basal layer of the human epidermis, where they attach to the basement membrane and replicate at a rate proportionate to that of keratinocytes, maintaining a life-long stable ratio. In this study, we report that coculturing melanocytes with keratinocytes up-regulated CCN3, a matricellular protein that we subsequently found to be critical for the spatial localization of melanocytes to the basement membrane. CCN3 knockdown cells were dissociated either upward to the suprabasal layers of the epidermis or downward into the dermis. The overexpression of CCN3 increased adhesion to collagen type IV, the major component of the basement membrane. As the receptor responsible for CCN3-mediated melanocyte localization, we identified discoidin domain receptor 1 (DDR1), a receptor tyrosine kinase that acts as a collagen IV adhesion receptor. DDR1 knockdown decreased melanocyte adhesion to collagen IV and shifted melanocyte localization in a manner similar to CCN3 knockdown. These results demonstrate an intricate and necessary communication between keratinocytes and melanocytes in maintaining normal epidermal homeostasis.

Original languageEnglish (US)
Pages (from-to)563-569
Number of pages7
JournalJournal of Cell Biology
Volume175
Issue number4
DOIs
StatePublished - Nov 20 2006

ASJC Scopus subject areas

  • Cell Biology

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    Fukunaga-Kalabis, M., Martinez, G., Liu, Z. J., Kalabis, J., Mrass, P., Weninger, W., Firth, S. M., Planque, N., Perbal, B., & Herlyn, M. (2006). CCN3 controls 3D spatial localization of melanocytes in the human skin through DDR1. Journal of Cell Biology, 175(4), 563-569. https://doi.org/10.1083/jcb.200602132