Catalytic properties of RNase BN/RNase Z from Escherichia coli. RNase BN is both an exo- and endoribonuclease

Tanmay Dutta, Murray P Deutscher

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Processing of the 3′ terminus of tRNA in many organisms is carried out by an endoribonuclease termed RNase Z or 3′-tRNase, which cleaves after the discriminator nucleotide to allow addition of the universal- CCA sequence. In some eubacteria, such as Escherichia coli, the -CCA sequence is encoded in all known tRNA genes. Nevertheless, an RNase Z homologue (RNase BN) is still present, even though its action is not needed for tRNA maturation. To help identify which RNA molecules might be potential substrates for RNase BN, we carried out a detailed examination of its specificity and catalytic potential using a variety of synthetic substrates. We show here that RNase BN is active on both double- and single-stranded RNA but that duplex RNA is preferred. The enzyme displays a profound base specificity, showing no activity on runs of C residues. RNase BN is strongly inhibited by the presence of a 3′-CCA sequence or a 3′-phosphoryl group. Digestion by RNase BN leads to 3-mers as the limit products, but the rate slows on molecules shorter than 10 nucleotides in length. Most interestingly, RNase BN acts as a distributive exoribonuclease on some substrates, releasing mononucleotides and a ladder of digestion products. However, RNase BN also cleaves endonucleolytically, releasing 3′ fragments as short as 4 nucleotides. Although the presence of a 3′-phosphoryl group abolishes exoribonuclease action, it has no effect on the endoribonucleolytic cleavages. These data suggest that RNase BN may differ from other members of the RNase Z family, and they provide important information to be considered in identifying a physiological role for this enzyme.

Original languageEnglish
Pages (from-to)15425-15431
Number of pages7
JournalJournal of Biological Chemistry
Volume284
Issue number23
DOIs
StatePublished - Jun 5 2009

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Exoribonucleases
Endoribonucleases
Escherichia coli
Transfer RNA
Nucleotides
RNA
Digestion
Substrates
Eosinophil Cationic Protein
Molecules
Discriminators
Double-Stranded RNA
ribonuclease BN
RNase Z
Ladders
Enzymes
Genes
Bacteria

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Catalytic properties of RNase BN/RNase Z from Escherichia coli. RNase BN is both an exo- and endoribonuclease. / Dutta, Tanmay; Deutscher, Murray P.

In: Journal of Biological Chemistry, Vol. 284, No. 23, 05.06.2009, p. 15425-15431.

Research output: Contribution to journalArticle

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