Ca2+ measurements in skinned cardiac fibers: Effects of Mg2+ on Ca2+ activation of force and fiber ATPase

Keri Allen, Yuan Yuan Xu, W. Glenn Kerrick

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In contrast to previous studies, a new fluorescent method was used to accurately determine the Ca2+ concentration in test solutions used to activate skinned rat cardiac cells. This method used the calcium green-2 fluorescent indicator, which is shown to change its fluorescence over the Ca2+ range responsible for Ca2+ activation of force and ATPase. The dissociation constant (K(d)) of calcium green-2 for Ca2+ was determined for three different Mg2+ concentrations in solutions similar to those used in the experiment. Increasing Mg2+ concentration from 1.0 to 8.0 mM had no significant effect on the Ca2+ sensitivity of either force or actomyosin ATPase activity, in contrast to previous reported studies on force. The ATPase activity was activated at lower Ca2+ concentration than the force. The ratio (ATPase/force) is proportional to the dissociation rate of force- generating myosin cross bridges and decreased during Ca2+ activation. These findings are consistent with the hypothesis that cardiac muscle contraction is activated by a single Ca2+-specific binding site on troponin C.

Original languageEnglish
Pages (from-to)180-185
Number of pages6
JournalJournal of Applied Physiology
Volume88
Issue number1
StatePublished - Jan 1 2000

Fingerprint

Adenosine Triphosphatases
Myosins
Troponin C
Muscle Contraction
Myocardium
Fluorescence
Binding Sites
calcium green

Keywords

  • Actomyosin
  • Binding constant
  • Calcium
  • Dissociation constant
  • Magnesium

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Ca2+ measurements in skinned cardiac fibers : Effects of Mg2+ on Ca2+ activation of force and fiber ATPase. / Allen, Keri; Xu, Yuan Yuan; Kerrick, W. Glenn.

In: Journal of Applied Physiology, Vol. 88, No. 1, 01.01.2000, p. 180-185.

Research output: Contribution to journalArticle

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