Ca2+-induced Ca2+ release in insulin-secreting cells

Md Shahidul Islam, Patrik Rorsman, Per Olof Berggren

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

The sulphydryl reagent thimerosal (50 μM) released Ca2+ from a non-mitochondrial intracellular Ca2+ pool in a dose-dependent manner in permeabilized insulin-secreting RINm5F cells. This release was reversed after addition of the reducing agent dithiothreitol, Ca2+ was released from an Ins(1,4,5)P3-insensitive pool, since release was observed even after depletion of the Ins(1,4,5)P3-sensitive pool by a supramaximal dose of Ins(2,4,5)P3 or thapsigargin. The Ins(1,4,5)P3-sensitive pool remained essentially unaltered by thimerosal. Thimerosal-induced Ca2+ release was potentiated by caffeine. These findings suggest the existence of Ca2+-induced Ca2+ release also in insulin-secreting cells.

Original languageEnglish (US)
Pages (from-to)287-291
Number of pages5
JournalFEBS letters
Volume296
Issue number3
DOIs
StatePublished - Jan 27 1992

Keywords

  • Ca-induced Ca release
  • Insulin-secreting cell
  • Intracellular Ca transport
  • Permeabilized cell
  • Sulphydryl reagent
  • Thimerosal

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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    Islam, M. S., Rorsman, P., & Berggren, P. O. (1992). Ca2+-induced Ca2+ release in insulin-secreting cells. FEBS letters, 296(3), 287-291. https://doi.org/10.1016/0014-5793(92)80306-2