Abstract
The sulphydryl reagent thimerosal (50 μM) released Ca2+ from a non-mitochondrial intracellular Ca2+ pool in a dose-dependent manner in permeabilized insulin-secreting RINm5F cells. This release was reversed after addition of the reducing agent dithiothreitol, Ca2+ was released from an Ins(1,4,5)P3-insensitive pool, since release was observed even after depletion of the Ins(1,4,5)P3-sensitive pool by a supramaximal dose of Ins(2,4,5)P3 or thapsigargin. The Ins(1,4,5)P3-sensitive pool remained essentially unaltered by thimerosal. Thimerosal-induced Ca2+ release was potentiated by caffeine. These findings suggest the existence of Ca2+-induced Ca2+ release also in insulin-secreting cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 287-291 |
| Number of pages | 5 |
| Journal | FEBS letters |
| Volume | 296 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jan 27 1992 |
Keywords
- Ca-induced Ca release
- Insulin-secreting cell
- Intracellular Ca transport
- Permeabilized cell
- Sulphydryl reagent
- Thimerosal
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Islam, M. S., Rorsman, P., & Berggren, P. O. (1992). Ca2+-induced Ca2+ release in insulin-secreting cells. FEBS letters, 296(3), 287-291. https://doi.org/10.1016/0014-5793(92)80306-2