Abstract
The sulphydryl reagent thimerosal (50 μM) released Ca2+ from a non-mitochondrial intracellular Ca2+ pool in a dose-dependent manner in permeabilized insulin-secreting RINm5F cells. This release was reversed after addition of the reducing agent dithiothreitol, Ca2+ was released from an Ins(1,4,5)P3-insensitive pool, since release was observed even after depletion of the Ins(1,4,5)P3-sensitive pool by a supramaximal dose of Ins(2,4,5)P3 or thapsigargin. The Ins(1,4,5)P3-sensitive pool remained essentially unaltered by thimerosal. Thimerosal-induced Ca2+ release was potentiated by caffeine. These findings suggest the existence of Ca2+-induced Ca2+ release also in insulin-secreting cells.
| Original language | English |
|---|---|
| Pages (from-to) | 287-291 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 296 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jan 27 1992 |
| Externally published | Yes |
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Keywords
- Ca-induced Ca release
- Insulin-secreting cell
- Intracellular Ca transport
- Permeabilized cell
- Sulphydryl reagent
- Thimerosal
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Ca2+-induced Ca2+ release in insulin-secreting cells. / Islam, Md Shahidul; Rorsman, Patrik; Berggren, Per Olof.
In: FEBS Letters, Vol. 296, No. 3, 27.01.1992, p. 287-291.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ca2+-induced Ca2+ release in insulin-secreting cells
AU - Islam, Md Shahidul
AU - Rorsman, Patrik
AU - Berggren, Per Olof
PY - 1992/1/27
Y1 - 1992/1/27
N2 - The sulphydryl reagent thimerosal (50 μM) released Ca2+ from a non-mitochondrial intracellular Ca2+ pool in a dose-dependent manner in permeabilized insulin-secreting RINm5F cells. This release was reversed after addition of the reducing agent dithiothreitol, Ca2+ was released from an Ins(1,4,5)P3-insensitive pool, since release was observed even after depletion of the Ins(1,4,5)P3-sensitive pool by a supramaximal dose of Ins(2,4,5)P3 or thapsigargin. The Ins(1,4,5)P3-sensitive pool remained essentially unaltered by thimerosal. Thimerosal-induced Ca2+ release was potentiated by caffeine. These findings suggest the existence of Ca2+-induced Ca2+ release also in insulin-secreting cells.
AB - The sulphydryl reagent thimerosal (50 μM) released Ca2+ from a non-mitochondrial intracellular Ca2+ pool in a dose-dependent manner in permeabilized insulin-secreting RINm5F cells. This release was reversed after addition of the reducing agent dithiothreitol, Ca2+ was released from an Ins(1,4,5)P3-insensitive pool, since release was observed even after depletion of the Ins(1,4,5)P3-sensitive pool by a supramaximal dose of Ins(2,4,5)P3 or thapsigargin. The Ins(1,4,5)P3-sensitive pool remained essentially unaltered by thimerosal. Thimerosal-induced Ca2+ release was potentiated by caffeine. These findings suggest the existence of Ca2+-induced Ca2+ release also in insulin-secreting cells.
KW - Ca-induced Ca release
KW - Insulin-secreting cell
KW - Intracellular Ca transport
KW - Permeabilized cell
KW - Sulphydryl reagent
KW - Thimerosal
UR - http://www.scopus.com/inward/record.url?scp=0026565989&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026565989&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(92)80306-2
DO - 10.1016/0014-5793(92)80306-2
M3 - Article
VL - 296
SP - 287
EP - 291
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -