Casitone-dependent transcriptional regulation of the prtP and prtM genes in the natural isolate lactobacillus paracasei subsp. paracasei

I. Paštar; J. Begović; J. Lozo; L. Topisirović; N. Golić

doi:10.1007/BF02932186

Casitone-dependent transcriptional regulation of the prtP and prtM genes in the natural isolate lactobacillus paracasei subsp. paracasei

I. Paštar, J. Begović, J. Lozo, L. Topisirović, N. Golić

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The prtP-prtM intergenic region of Lactobacillus paracasei subsp. paracasei BGHN14 was cloned and sequenced. The nucleotide sequence of the prtP-prtM intergenic region in BGHN14, containing divergently orientated P_prtP and P_prtM/promoters, was shorter by 35 bp in comparison with that in lactococci. The nucleotide sequence involved in casitone-dependent transcriptional regulation of the lactococcal prt genes was not found in the BGHN14. The activity of P_prtM in L. lactis NZ9000 was very low and insignificantly changed in the presence of casitone, whereas P_prtP was completely inactive. When L. casei ATCC393^T was used as host, both P_prtP and P_prtM were active and strongly regulated by casitone. The results strongly indicate that the mechanisms of the casitone-dependent regulation of the prt genes in BGHN14 and lactococci are different.

Original language	English (US)
Pages (from-to)	577-584
Number of pages	8
Journal	Folia Microbiologica
Volume	52
Issue number	6
DOIs	https://doi.org/10.1007/BF02932186
State	Published - 2007
Externally published	Yes

ASJC Scopus subject areas

Microbiology

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10.1007/BF02932186

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@article{b88093c5d4cd49129ff298c6288b7f2b,

title = "Casitone-dependent transcriptional regulation of the prtP and prtM genes in the natural isolate lactobacillus paracasei subsp. paracasei",

abstract = "The prtP-prtM intergenic region of Lactobacillus paracasei subsp. paracasei BGHN14 was cloned and sequenced. The nucleotide sequence of the prtP-prtM intergenic region in BGHN14, containing divergently orientated PprtP and PprtM/promoters, was shorter by 35 bp in comparison with that in lactococci. The nucleotide sequence involved in casitone-dependent transcriptional regulation of the lactococcal prt genes was not found in the BGHN14. The activity of PprtM in L. lactis NZ9000 was very low and insignificantly changed in the presence of casitone, whereas PprtP was completely inactive. When L. casei ATCC393T was used as host, both PprtP and PprtM were active and strongly regulated by casitone. The results strongly indicate that the mechanisms of the casitone-dependent regulation of the prt genes in BGHN14 and lactococci are different.",

author = "I. Pa{\v s}tar and J. Begovi{\'c} and J. Lozo and L. Topisirovi{\'c} and N. Goli{\'c}",

note = "Funding Information: This work was funded by the Ministry of Science and Environmental Protection of the Republic of Serbia, grant no. 143036. We are grateful to O. Kuipers for kindly providing plasmid pNZ273 and G. P{\'e}rez-Mart{\'i}nez for Lactobacillus casei ATCC 393T.",

year = "2007",

doi = "10.1007/BF02932186",

language = "English (US)",

volume = "52",

pages = "577--584",

journal = "Folia Microbiologica",

issn = "0015-5632",

publisher = "Springer Netherlands",

number = "6",

}

TY - JOUR

T1 - Casitone-dependent transcriptional regulation of the prtP and prtM genes in the natural isolate lactobacillus paracasei subsp. paracasei

AU - Paštar, I.

AU - Begović, J.

AU - Lozo, J.

AU - Topisirović, L.

AU - Golić, N.

N1 - Funding Information: This work was funded by the Ministry of Science and Environmental Protection of the Republic of Serbia, grant no. 143036. We are grateful to O. Kuipers for kindly providing plasmid pNZ273 and G. Pérez-Martínez for Lactobacillus casei ATCC 393T.

PY - 2007

Y1 - 2007

N2 - The prtP-prtM intergenic region of Lactobacillus paracasei subsp. paracasei BGHN14 was cloned and sequenced. The nucleotide sequence of the prtP-prtM intergenic region in BGHN14, containing divergently orientated PprtP and PprtM/promoters, was shorter by 35 bp in comparison with that in lactococci. The nucleotide sequence involved in casitone-dependent transcriptional regulation of the lactococcal prt genes was not found in the BGHN14. The activity of PprtM in L. lactis NZ9000 was very low and insignificantly changed in the presence of casitone, whereas PprtP was completely inactive. When L. casei ATCC393T was used as host, both PprtP and PprtM were active and strongly regulated by casitone. The results strongly indicate that the mechanisms of the casitone-dependent regulation of the prt genes in BGHN14 and lactococci are different.

AB - The prtP-prtM intergenic region of Lactobacillus paracasei subsp. paracasei BGHN14 was cloned and sequenced. The nucleotide sequence of the prtP-prtM intergenic region in BGHN14, containing divergently orientated PprtP and PprtM/promoters, was shorter by 35 bp in comparison with that in lactococci. The nucleotide sequence involved in casitone-dependent transcriptional regulation of the lactococcal prt genes was not found in the BGHN14. The activity of PprtM in L. lactis NZ9000 was very low and insignificantly changed in the presence of casitone, whereas PprtP was completely inactive. When L. casei ATCC393T was used as host, both PprtP and PprtM were active and strongly regulated by casitone. The results strongly indicate that the mechanisms of the casitone-dependent regulation of the prt genes in BGHN14 and lactococci are different.

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Casitone-dependent transcriptional regulation of the prtP and prtM genes in the natural isolate lactobacillus paracasei subsp. paracasei

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