Abstract
The prtP-prtM intergenic region of Lactobacillus paracasei subsp. paracasei BGHN14 was cloned and sequenced. The nucleotide sequence of the prtP-prtM intergenic region in BGHN14, containing divergently orientated PprtP and PprtM/promoters, was shorter by 35 bp in comparison with that in lactococci. The nucleotide sequence involved in casitone-dependent transcriptional regulation of the lactococcal prt genes was not found in the BGHN14. The activity of PprtM in L. lactis NZ9000 was very low and insignificantly changed in the presence of casitone, whereas PprtP was completely inactive. When L. casei ATCC393T was used as host, both PprtP and PprtM were active and strongly regulated by casitone. The results strongly indicate that the mechanisms of the casitone-dependent regulation of the prt genes in BGHN14 and lactococci are different.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 577-584 |
| Number of pages | 8 |
| Journal | Folia Microbiologica |
| Volume | 52 |
| Issue number | 6 |
| DOIs | |
| State | Published - 2007 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Microbiology
Cite this
Casitone-dependent transcriptional regulation of the prtP and prtM genes in the natural isolate lactobacillus paracasei subsp. paracasei. / Pastar, Irena; Begović, J.; Lozo, J.; Topisirović, L.; Golić, N.
In: Folia Microbiologica, Vol. 52, No. 6, 2007, p. 577-584.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Casitone-dependent transcriptional regulation of the prtP and prtM genes in the natural isolate lactobacillus paracasei subsp. paracasei
AU - Pastar, Irena
AU - Begović, J.
AU - Lozo, J.
AU - Topisirović, L.
AU - Golić, N.
PY - 2007
Y1 - 2007
N2 - The prtP-prtM intergenic region of Lactobacillus paracasei subsp. paracasei BGHN14 was cloned and sequenced. The nucleotide sequence of the prtP-prtM intergenic region in BGHN14, containing divergently orientated PprtP and PprtM/promoters, was shorter by 35 bp in comparison with that in lactococci. The nucleotide sequence involved in casitone-dependent transcriptional regulation of the lactococcal prt genes was not found in the BGHN14. The activity of PprtM in L. lactis NZ9000 was very low and insignificantly changed in the presence of casitone, whereas PprtP was completely inactive. When L. casei ATCC393T was used as host, both PprtP and PprtM were active and strongly regulated by casitone. The results strongly indicate that the mechanisms of the casitone-dependent regulation of the prt genes in BGHN14 and lactococci are different.
AB - The prtP-prtM intergenic region of Lactobacillus paracasei subsp. paracasei BGHN14 was cloned and sequenced. The nucleotide sequence of the prtP-prtM intergenic region in BGHN14, containing divergently orientated PprtP and PprtM/promoters, was shorter by 35 bp in comparison with that in lactococci. The nucleotide sequence involved in casitone-dependent transcriptional regulation of the lactococcal prt genes was not found in the BGHN14. The activity of PprtM in L. lactis NZ9000 was very low and insignificantly changed in the presence of casitone, whereas PprtP was completely inactive. When L. casei ATCC393T was used as host, both PprtP and PprtM were active and strongly regulated by casitone. The results strongly indicate that the mechanisms of the casitone-dependent regulation of the prt genes in BGHN14 and lactococci are different.
UR - http://www.scopus.com/inward/record.url?scp=39449139724&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=39449139724&partnerID=8YFLogxK
U2 - 10.1007/BF02932186
DO - 10.1007/BF02932186
M3 - Article
VL - 52
SP - 577
EP - 584
JO - Folia Microbiologica
JF - Folia Microbiologica
SN - 0015-5632
IS - 6
ER -