Casitone-dependent transcriptional regulation of the prtP and prtM genes in the natural isolate lactobacillus paracasei subsp. paracasei

Irena Pastar, J. Begović, J. Lozo, L. Topisirović, N. Golić

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The prtP-prtM intergenic region of Lactobacillus paracasei subsp. paracasei BGHN14 was cloned and sequenced. The nucleotide sequence of the prtP-prtM intergenic region in BGHN14, containing divergently orientated PprtP and PprtM/promoters, was shorter by 35 bp in comparison with that in lactococci. The nucleotide sequence involved in casitone-dependent transcriptional regulation of the lactococcal prt genes was not found in the BGHN14. The activity of PprtM in L. lactis NZ9000 was very low and insignificantly changed in the presence of casitone, whereas PprtP was completely inactive. When L. casei ATCC393T was used as host, both PprtP and PprtM were active and strongly regulated by casitone. The results strongly indicate that the mechanisms of the casitone-dependent regulation of the prt genes in BGHN14 and lactococci are different.

Original languageEnglish (US)
Pages (from-to)577-584
Number of pages8
JournalFolia Microbiologica
Volume52
Issue number6
DOIs
StatePublished - 2007
Externally publishedYes

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Lactococcus
Intergenic DNA
Genes
casein hydrolysate
Lactobacillus paracasei

ASJC Scopus subject areas

  • Microbiology

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Casitone-dependent transcriptional regulation of the prtP and prtM genes in the natural isolate lactobacillus paracasei subsp. paracasei. / Pastar, Irena; Begović, J.; Lozo, J.; Topisirović, L.; Golić, N.

In: Folia Microbiologica, Vol. 52, No. 6, 2007, p. 577-584.

Research output: Contribution to journalArticle

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AU - Pastar, Irena

AU - Begović, J.

AU - Lozo, J.

AU - Topisirović, L.

AU - Golić, N.

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N2 - The prtP-prtM intergenic region of Lactobacillus paracasei subsp. paracasei BGHN14 was cloned and sequenced. The nucleotide sequence of the prtP-prtM intergenic region in BGHN14, containing divergently orientated PprtP and PprtM/promoters, was shorter by 35 bp in comparison with that in lactococci. The nucleotide sequence involved in casitone-dependent transcriptional regulation of the lactococcal prt genes was not found in the BGHN14. The activity of PprtM in L. lactis NZ9000 was very low and insignificantly changed in the presence of casitone, whereas PprtP was completely inactive. When L. casei ATCC393T was used as host, both PprtP and PprtM were active and strongly regulated by casitone. The results strongly indicate that the mechanisms of the casitone-dependent regulation of the prt genes in BGHN14 and lactococci are different.

AB - The prtP-prtM intergenic region of Lactobacillus paracasei subsp. paracasei BGHN14 was cloned and sequenced. The nucleotide sequence of the prtP-prtM intergenic region in BGHN14, containing divergently orientated PprtP and PprtM/promoters, was shorter by 35 bp in comparison with that in lactococci. The nucleotide sequence involved in casitone-dependent transcriptional regulation of the lactococcal prt genes was not found in the BGHN14. The activity of PprtM in L. lactis NZ9000 was very low and insignificantly changed in the presence of casitone, whereas PprtP was completely inactive. When L. casei ATCC393T was used as host, both PprtP and PprtM were active and strongly regulated by casitone. The results strongly indicate that the mechanisms of the casitone-dependent regulation of the prt genes in BGHN14 and lactococci are different.

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