Cascade of bioreactors in series for conversion of 3-phospho-D-glycerate into D-ribulose-1,5-bisphosphate

Kinetic parameters of enzymes and operation variables

Sumana Bhattacharya, Marc Schiavone, James Gomes, Sanjoy K Bhattacharya

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A novel scheme employing enzymatic catalysts is described enabling conversion of D-ribulose-1,5-bisphosphate (RuBP) from 3-phospho-D-glycerate (3-PGA) without loss of carbon. Bioreactors harboring immobilized enzymes namely, phosphoglycerate kinase (PGK), glycerate phosphate dehydrogenase, triose phosphate isomerase (TIM), aldolase, transketolase (TKL), phosphatase (PTASE/FP), epimerase (EMR) and phosphoribulokinase (PRK), in accordance with this novel scheme were employed. These reactors were designed and constructed based on simulations carried out to study their performance under various operational conditions and allowed production of about 56 ± 3% RuBP from 3-PGA. This method of synthesis of RuBP from 3-PGA employing immobilized enzyme bioreactors may be used for continuous regeneration of RuBP in biocatalytic carbon dioxide fixation processes from emissions where RuBP acts as acceptor of carbon dioxide to produce 3-PGA, rendering the fixation process continuous.

Original languageEnglish
Pages (from-to)203-217
Number of pages15
JournalJournal of Biotechnology
Volume111
Issue number2
DOIs
StatePublished - Jul 15 2004
Externally publishedYes

Fingerprint

Bioreactors
Kinetic parameters
Carbon dioxide
Phosphates
Enzymes
Phosphatases
Immobilized Enzymes
Glycerate dehydrogenase
Carbon Dioxide
Catalysts
Carbon
Transketolase
Triose-Phosphate Isomerase
Phosphoglycerate Kinase
Racemases and Epimerases
Carbon Cycle
Fructose-Bisphosphate Aldolase
Phosphoric Monoester Hydrolases
Regeneration
Oxidoreductases

Keywords

  • 3-PGA
  • Enzymes
  • RuBP

ASJC Scopus subject areas

  • Biotechnology

Cite this

Cascade of bioreactors in series for conversion of 3-phospho-D-glycerate into D-ribulose-1,5-bisphosphate : Kinetic parameters of enzymes and operation variables. / Bhattacharya, Sumana; Schiavone, Marc; Gomes, James; Bhattacharya, Sanjoy K.

In: Journal of Biotechnology, Vol. 111, No. 2, 15.07.2004, p. 203-217.

Research output: Contribution to journalArticle

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