Cartilage interstitial fluid load support in unconfined compression following enzymatic digestion

Ines M. Basalo, Robert L. Mauck, Terri Ann N. Kelly, Steven B. Nicoll, Faye H. Chen, Clark T. Hung, Gerard A. Ateshian

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Interstitial fluid pressurization plays an important role in cartilage biomechanics and is believed to be a primary mechanism of load support in synovial joints. The objective of this study was to investigate the effects of enzymatic degradation on the interstitial fluid load support mechanism of articular cartilage in unconfined compression. Thirty-seven immature bovine cartilage plugs were tested in unconfined compression before and after enzymatic digestion. The peak fluid load support decreased significantly (p < 0.0001) from 84 ± 10% to 53 ± 19% and from 80 ± 10% to 46 ± 21% after 18-hours digestion with 1.0 u/mg-wet-weight and 0.7 u/mg-wet-weight of collagenase, respectively. Treatment with 0.1 u/ml of chondroitinase ABC for 24 hours also significantly reduced the peak fluid load support from 83 ± 12% to 48 ± 16% (p < 0.0001). The drop in interstitial fluid load support following enzymatic treatment is believed to result from a decrease in the ratio of tensile to compressive moduli of the solid matrix.

Original languageEnglish (US)
Pages (from-to)779-786
Number of pages8
JournalJournal of biomechanical engineering
Volume126
Issue number6
DOIs
StatePublished - Dec 2004

ASJC Scopus subject areas

  • Biomedical Engineering
  • Physiology (medical)

Fingerprint Dive into the research topics of 'Cartilage interstitial fluid load support in unconfined compression following enzymatic digestion'. Together they form a unique fingerprint.

  • Cite this

    Basalo, I. M., Mauck, R. L., Kelly, T. A. N., Nicoll, S. B., Chen, F. H., Hung, C. T., & Ateshian, G. A. (2004). Cartilage interstitial fluid load support in unconfined compression following enzymatic digestion. Journal of biomechanical engineering, 126(6), 779-786. https://doi.org/10.1115/1.1824123